The invention belongs to the technical field of
plant germplasm resource evaluation, and particularly relates to a method for constructing mango core
germplasm based on SSR fluorescent labeling. According to the method, mango sample
genetic diversity analysis is carried out through an SSR
molecular marker technology, and related parameters of
germplasm resource molecular data are calculated; carrying out overall clustering sampling on the mangoes by using a UPGMA clustering method according to the
genetic distance, and specifically, adopting
a site-first sampling strategy and Neiamp; and the Li
genetic distance is used for gradually clustering and sampling the whole mango germplasm resources to construct the core germplasm. The constructed mango core germplasm has rich
genetic diversity, the number of lost alleles is small, the
genetic diversity of the original germplasm can be represented to the maximum extent with the minimum number of resources, and a theoretical basis is provided for preservation of mango germplasm, breeding of new varieties and development and utilization of the mango germplasm.