The invention relates to the technical field of laying duck breeding, and discloses a traditional Chinese medicine formula for breeding and enhancing laying duck disease resistance and a preparation method thereof. The laying duck feed is prepared from the following raw materials in parts by weight: corn, wheat, soybean meal, wheat bran, mountain flour, calcium bicarbonate, edible salt, methionine, herba haleniae, honeysuckle flower, fructus forsythiae, radix rehmanniae, fructus crataegi, fructus hordei germinatus, rhizoma phragmitis, rhizoma polygoni cuspidati, radix scutellariae, indigo naturalis, massa medicata fermentata, complex enzyme, and complex mineral. According to the feed disclosed by the invention, various traditional chinese medicines such as herba haleniae, honeysuckle flower, fructus forsythiae, radix rehmanniae, fructus crataegi, fructus hordei germinatus, rhizoma phragmitis, rhizoma polygoni cuspidati, radix scutellariae, indigo naturalis and massa medicata fermentataare added, are matched scientifically, and complement one other, so as to adjust various organs of the whole body of a young laying duck, promote metabolism, improve the immunity, disease resistanceand the ability of resisting stress, prevent deficiency disease, and then improve the disease resistance of the laying duck; corn, wheat, soybean meal, wheat bran and the like are taken as basic materials to ensure the supply of nutritions such as protein in a laying duck growth course.