40 results about "Sterile water solution" patented technology

A packaging arrangement for the containment of at least one hydrophilic contact lens in a sterile aqueous solution. More specifically, pursuant to the packaging arrangement, a plurality of disposable hydrophilic contact lenses are contained in a specific number of individual packaging arrangements collectively housed in a box-like container or carton so as to provide a specified or essentially measured supply of contact lenses for use by a consumer over a predetermined period of time.

The invention relates to a reagent and a method for the quick release of nucleic acid. The reagent for releasing the nucleic acid is prepared by dissolving 0.01 to 0.5 milliliter per liter (the concentration of the surface bioactive peptide in the sterile aqueous solution of KCl is 0.01 to 0.5 mM/L) of the surface bioactive peptide in 50 to 200 milliliter per liter of sterile aqueous solution of KCl, and adding 0.01 to 2 percent (the concentration of the SDS, the LLS or the Chelex-100 in sterile water is 0.01 to 2 percent) of the SDS, the LLS or the Chelex-100, and 0.05 to 1 percent (volume) of ethanol, wherein the percentages are calculated based on the volume of the sterile water. The method for releasing the nucleic acid comprises the following steps of: taking the reagent for releasing the nucleic acid, and performing equivalent dilution on the reagent by using the sterile water according to different experimental purposes; balancing the diluted reagent to the room temperature, and then mixing the reagent uniformly; sub-packaging the mixture into a sample tube to be tested; adding a sample to be treated in the sample tube; and absorbing and beating the sample repeatedly for 5 to 10 times by a pipettor. The reagent for releasing the nucleic acid is safe to use and has a low production cost; and the method for releasing the nucleic acid is simple and convenient to operate.

The invention relates to a protecting agent for free DNA in plasma and belongs to the biotechnical field. The protecting agent for free DNA in plasma is a sterile aqueous solution comprising the following components: a 50-600 g/L preservative, a 30-100 g/L nuclease inhibitor, a 20-200 g/L metabolic inhibitor and a 1-120 g/L anti-adsorbent. By mixing collected blood with the protecting agent in a proportion of (5-1000): 1, the free DNA of blood can be kept for 14 days in a constant temperature condition, the free DNA of blood can be kept for 4 days at 37 DEG C, and the free DNA of blood can betransported continuously for two days. By optimizing the components and the contents thereof, free nucleic acid in blood can be stored and extracted effectively, and meanwhile, nucleic acid doped intoblood cells as a result of splitting decomposition of hemocyte is avoided after blood is extracted.

An improved medical treatment and medicine is provided to quickly and safely resolve HIV and other microbial infections. The inexpensive medicine can be self administered and maintained for the prescribed time. The attractive medicine comprises an antimicrobial concentrate comprising microbe inhibitors, phytochemicals or isolates. Desirably, the effective medicine comprises a surfactant and an aqueous carrier or solvent and a nutrient. In the preferred form, the medicine comprises: Echinacea and Commiphora myrrha phytochemicals, benzalkonium chloride, a sterile water solution, and folic acid.

The invention discloses a chemical method for treating epidemic disease infected animal corpse. The method is mainly characterized in that alkali hydrolysis at a proper high temperature is utilized to converse pathogenic microorganisms, and proteins, nucleic acids and lipid of all cells and issues in animal bodies into a neutral aseptic aqueous solution with small peptides, amino acids, saccharides and soaps, and into bone residues. During the process, alkali itself is consumed by generating saline hydrolytic solution, and an only solid byproduct is mineral compounds of vertebrate bones and teeth. The solid residues are very soft to be easily crushed into a calcium-phosphor powder.

The invention discloses a purely-natural all-fruit sea-buckthorn chewable tablet. A preparation method of the purely-natural all-fruit sea-buckthorn chewable tablet comprises the following steps: (1) carrying out drying treatment on sea-buckthorn fruits; (2) carrying out tabletting treatment; (3) carrying out anti-bacterial and anti-mildew film laminating treatment; and (4) drying and sterilizing to obtain the purely-natural all-fruit sea-buckthorn chewable tablet. The anti-bacterial and anti-mildew film laminating treatment in the step (3) is finished by adopting an aqueous sterile solution containing 2-5% of licorice extract and 1-2% of water-soluble oligomeric chitosan to soak for 25-35 seconds and then draining. The purely-natural all-fruit sea-buckthorn chewable tablet without containing chemical additives has the advantages that the sea-buckthorn fruits can be intuitively seen, a user can obtain single-particle-restored sea-buckthorn fruits by brewing with boiling water, the convenience in eating, the pure natural property and the visibility of the chewable tablet are better than those of an existing sea-buckthorn product on the market; and simultaneously, based on the fact that the sea buckthorn is rich in vitamin C, the usage amount of the tablets can be determined by fully considering the recommended amount of the vitamin C for a human body on the production specifications, so that the need of the human body on the vitamin C can be met by only taking 1-2 tablets every day.

The invention relates to the field of foods, and discloses a preparation method of canned sauries low in histamine. The preparation method comprises the following steps of performing unfreezing, performing histamine removing treatment, performing dicing and cleaning, performing canning and cooking, preparing materials, performing exhausting, and performing sealing and sterilization. In the steps of performing histamine removing treatment and performing dicing and cleaning, the sauries are treated with a histamine treating agent, and the histamine treating agent is a bacteria-free water solution containing shell calcined powder, lecithin, soluble chitosan and lysozymes. The histamine treating agent not only can adsorb and remove the histamine, but also can kill microorganisms generating thehistamine, restrains bioactivity of the microorganisms generating the histamine, and is notable in effect of reducing the content of histamine. The raw materials are easy to obtain, the preparation method is simple, organic solvents are not used, a penetrant and a dispersing agent do not need to be added additionally, the content of the histamine is reduced, and besides, the content of calcium inproducts can also be increased. The nutrient components of the products are enriched, and the histamine treating agent can be used as a fresh keeping agent. The preparation technology is simple, thecost is low, and the preparation technology is suitable for industrialized production.

A method and apparatus for producing bacteria-free iodine-species-containing drinking water for farm animals under continuous dynamic water flow, comprising dissolving solid iodine into a first water flow to produce a saturated iodine species-containing aqueous solution at a pre-selected temperature; blending the saturated solution with a second water flow to produce a diluted iodine species bacterium-free aqueous solution; and providing the diluted solution as drinking water to the animals. Preferably, the iodine is dissolved in the first water flow to provide a saturated iodine species at a pre-selected temperature at a known concentration, which saturated solution is then blended into a mean water flow. The continuous flow of iodine species-containing water is fed to a farm animal drinking water distribution network with reduced risk of back-contamination by bacteria-containing water through the network. Other uses of the iodinated water are as a disinfectant, for example, in the food processing industry; fruit, vegetable and fish preservation; industrial, commercial cooling tower waters, sewage and waste water treatment; and as a nutrient as an iodine source for humans, livestock, fish and plants.

The invention belongs to the technical field of organisms and discloses a method for removing explant endophyte during plant tissue culturing. The cuttage soil can be disinfected by 50% of carbendazim, cuttage twigs are disinfected by sterile water solution containing 200 mg/L of amphotericin and 100 mg/L of streptomycin sulfate, and new semi-lignified twigs can be treated in advance by sterile water solution containing 100 mg/L of amphotericin and 50 mg/L of streptomycin sulfate. Accordingly, bacterial and fungi on the surface of and inside the explant such as aspen can be effectively removed, and sufficient vigor of the explant can be kept.

The invention relates to a method for separating and purifying stem cells from human umbilical cord blood. The method comprises the following steps: adopting a kit for separating and purifying human umbilical cord blood enchylema, thereby acquiring a mononuclear cell sample, wherein the kit comprises a reagent A and a reagent B, the reagent A is an erythrocyte precipitator and is composed of 6% hydroxyethyl starch solution and the reagent B is a cell separating medium, is prepared from saccharosan 400 and cardiografin and is a sterile aqueous solution; adopting a stem cell culture medium for culturing, multiplying and purifying the stem cells, wherein the stem cell culture medium is prepared by mixing DMEM/F12 stem cell culture medium and optimized additives. According to the invention, the umbilical cord blood stem cell in vitro culture is utilized and the components are added into the cell culture medium, so that the multiplication advantage of the mesenchymal stem cells is promoted, the growth of other cells is expelled and the cells are naturally purified; the recovery of the monocytes is more than or equal to 95%; the survival rate of the stem cells is more than or equal to 98%; the quantity of the mesenchymal stem cells is more than or equal to 5*10<7>; the quality requirement of clinic treatment is met.

There are provided a pharmaceutical raw material capable of exhibiting a uniform functionality and producing magnetic particle-containing drugs for diagnosis and medical treatments with a high reproducibility. The pharmaceutical raw material comprises a monodisperse colloid sterile aqueous solution of magnetic iron oxide fine particles having an average particle diameter of 5 to 30 nm, a saturation magnetization of 50 to 90 Am²/kg and a coercive force of 0.1 to 1.6 kA/m. The pharmaceutical raw material can be produced by forming magnetic iron oxide fine particles in the form of a colloid aqueous solution, purifying the resultant colloid aqueous solution of superparamagnetic iron oxide particles by water-washing and removing water-soluble salts by-produced upon the reaction from the reaction solution by an ordinary method, and replacing a dispersing medium of the purified colloid aqueous solution with ultrapure water.

The invention relates to the field of food, and discloses a preparation method of low-histamine coryphaena hippurus fillets. The preparation method comprises pretreatment, thawing, cleaning, slicing,secondary cleaning and freezing. In the cleaning step, a histamine treating agent is adopted, and the histamine treating agent is prepared from calcined shell powder, lecithin and sterile water. In the secondary cleaning step, a tetracycline adsorbent is adopted, and the tetracycline adsorbent is prepared from a sterile aqueous solution containing lignin, soluble chitosan and lysozyme. The preparation method can not only adsorb and remove histamine, but also kill histamine-producing microorganisms, inhibit the biological activity of the histamine-producing microorganisms, and remarkably reducehistamine content; and meanwhile, the preparation method can also remove tetracycline substances remaining in a culturing process. Raw materials are easy to obtain, and the preparation method is simple, uses no organic solvent, requires no additional penetrant or dispersant, increases calcium content of the product while reducing the histamine content, and enriches the nutrient components of theproduct.

The present invention relates to pharmaceutical compositions for the parenteral administration of melatonin in the form of sterile aqueous solutions, provided with good stability even if devoid of any stabilising excipients and having a concentration of melatonin up to high amounts, sufficient for an efficacious medical treatment.

The present invention is directed to a fermented milk production process in self-rehydrating container comprising a carbohydrate, a fermented milk product forming starter culture, milk powder and eventually other edible solutes, and self-rehydrating container adapted thereto. The container contains a semi-permeable membrane which allows the preparation of substantially sterile water solutions using osmotically driven filtration.

The invention provides a biocompatible, injectable sterile aqueous solution for use in high-intensity-ultrasound-energy-assisted surgery comprising antioxidants selected from the group consisting of ascorbic acid, glutathione and mixtures thereof in a buffered biocompatible solution for the reduction and limitation of reactive oxygen species.

The invention relates to a preparation method of radix puerariae yoghourt for reducing three highs and resisting tumors. The preparation method comprises the following steps of adding a puerarin extracting solution into yoghourt, wherein a preparation method of the puerarin extracting solution comprises the following specific steps: firstly, crushing radix puerariae to obtain radix puerariae powder with 16 meshes; secondly, uniformly mixing the dried radix puerariae powder with diatomite according to weight ratio of 1 to 1, filling an extracting pool with a mixture, and then adding 70 percent ethanol as an extracting agent, wherein the material-liquid ratio of the radix puerariae to the ethanol is 1:25; thirdly, using an automatic extracting apparatus to extract at the extraction temperature of 60DEG C for 20 minutes, and repeatedly extracting for two times; fourthly, concentrating the extracting solution by using a rotary evaporator, filtering the extracting solution and then adding the filtered solution into a sterile aqueous solution to obtain the puerarin extracting solution. According to the preparation method disclosed by the invention, the extraction rate of puerariae in the radix puerariae can be improved; in addition, the content of the puerariae is high, so that the yoghourt has health-care functions of improving cardio-cerebral blood circulation, expanding coronary artery, decreasing blood pressure, decreasing blood sugar, dispelling the effects of alcohol and protecting liver, resisting liver fibrosis and the like.

The present invention relates to the field of food and discloses a preparation method of low histamine scombrida fish fillets. The preparation method comprises the following steps: pretreating, thawing, washing, slicing, secondary washing and freezing. In the washing and secondary washing steps, the fish fillets are treated with a histamine treatment agent. The histamine treatment agent is a sterile aqueous solution of shell calcined powder, zeolite powder, lecithin, soluble chitosan and lysozyme. The histamine treatment agent can absorb and remove histamine, can also kill microorganisms producing the histamine, inhibits biological activities of the microorganisms producing the histamine, significantly reduces a histamine content, at the same time can remove heavy metals in fish meat, is easy to get raw materials and simple in the preparation method, does not use organic solvents, is free of extra additions of penetrants and dispersants, reduces the histamine content, at the same timealso increases a calcium content of products, enriches a nutritional content of the products, and can also be used as a preservative for use. The preparation method is simple in technology, low in costs and suitable for an industrialized production.

The invention discloses a preparation method of composite hydrogel, and a construction method of a cell microenvironment bionic system. The preparation method of the composite hydrogel comprises the following steps: mixing a gelatin solution, a sodium alginate solution, a glycerin solution, a glutaraldehyde solution, a phosphate buffer salt solution and a sterile aqueous solution according to a preset volume ratio to obtain a precursor solution the glycerol volume concentration being 1-5%; freezing the precursor solution to convert gelatin from sol to gel, and freezing glycerol to form pores; and adding a calcium chloride solution to carry out ionic crosslinking on sodium alginate in the gel to obtain the composite hydrogel, wherein the concentration of calcium chloride is associated with the rigidity of the composite hydrogel. The composite hydrogel prepared by the embodiment of the invention is good in forming, stable in mechanical property and uniform in pore structure, and can be used for simulating an extracellular matrix microenvironment in vitro.

An application method of Perilla frutescens essential oil in the normal temperature storage process of citrus comprises the following steps: (1) extracting a Perilla frutescens extracting solution; (2) preparing a cyclodextrin suspension; (3) mixing the cyclodextrin suspension with a span-80, adding the Perilla frutescens extracting solution and uniformly stirring; (4) filtering and separating themixed liquid to obtain cyclodextrin, standing and layering, and separating the two phases; adding ethanol into the cyclodextrin to obtain Perilla frutescens essential oil a; (5) taking the upper layer of the two-phase layer, removing the ethanol and the span-80 to obtain Perilla frutescens essential oil b; (6) mixing the Perilla frutescens essential oil a with the Perilla frutescens essential oilb to obtain Perilla frutescens essential oil c, and adding tween-80, adding sterile water, and fully uniformly stirring to obtain a Perilla frutescens essential oil product; (7) taking the Perilla frutescens essential oil product, adding a tween-80 sterile water solution to obtain a Perilla frutescens essential oil bacteriostatic agent; (8) spraying the Perilla frutescens essential oil bacteriostatic agent in a closed storage space for use. According to the method disclosed by the invention, the growth and propagation of Penicillium citrinum can be inhibited, and the preservation time of thecitrus is prolonged.