4 results about "Sterile water solution" patented technology

The pretreatment method of the resistance gene detection sample in the breeding waste comprises the following steps: suspending the breeding waste in sterile water, and treating the breeding waste for 1-2 hours by adopting a pretreatment agent I; the pretreating agent I is prepared from aluminum sulfate and ferrous sulfate according to the weight ratio of 5: (1-3); after centrifuging, adding a pretreating agent II into a solid phase for treating; the pretreatment agent II is a sterile aqueous solution composed of 8-12% w / w of PVP30 and 0.5-1.5% w / w of sodium acetate; after centrifugation, adding a pretreatment agent III into a solid phase for treatment; the pretreatment agent III is a sterile aqueous solution composed of potassium acetate with the concentration of 3-5 M and sodium chloride with the concentration of 0.5-0.8 M. According to the pretreatment method provided by the invention, DNA in the breeding waste can be effectively reserved, the content of DNA obtained by subsequent DNA extraction work is effectively increased, and detection of resistance genes in the breeding waste is facilitated.

A bone gel composition comprising cortical bone is provided. The cortical bone is made from cut pieces freeze-dried then ground into particles and demineralized then freeze-dried. A volume of the particles is placed in a solution of sterile water to create a mixture, the water volume being twice the second portion, the mixture is autoclaved under heat and pressure to form a gelatin, the resulting bone gel is placed in jars and kept at room temperature or frozen for later use. The cortical bone has the cut pieces having a width, a length and a thickness in the range of 1 to 4 mm. The cortical bone pieces are ground to a particle size up to 125 microns.

A solid cannabinoid composition comprising: (i) 0.3 to 10% by weight of one or more cannabinoids; (ii) 4 to 69% by weight of one or more nonionic surfactants having an HLB value of 10 to 25; (iii) at least 20% by weight of one or more carbohydrates selected from mannitol, sucrose, trehalose, and stachyose; and (iv) 0 to 5% by weight of water, wherein the combination of one or more nonionic surfactants and one or more carbohydrates accounts for at least 70% by weight of the composition. A sterile aqueous solution for use in medical treatment, comprising: (a) 0.1 to 10 mg / mL of one or more cannabinoids; (b) one or more nonionic surfactants having an HLB value of 10 to 25; (c) one or more carbohydrates selected from mannitol, sucrose, trehalose, and stachyose; and (d) at least 85% by weight of water, wherein one or more nonionic surfactants and one or more cannabinoids are present in the sterile aqueous solution in a weight ratio of 3:1 to 30:1, and one or more carbohydrates and one or more cannabinoids are present in the sterile aqueous solution in a weight ratio of 3:1 to 300:1.

The invention discloses a method for establishing a stable damp-heat ulcerative colitis animal model. A DSS, high-glucose and high-fat diet and artificial climate box combined induced damp-heat type ulcerative colitis model is adopted, and the method comprises the following steps: (1) high-glucose and high-fat diet: mice freely eat high-glucose and high-fat feed from the first day until an experiment is finished; (2) artificial climate box treatment: from the eleventh day, putting the mice into an artificial climate box with the temperature of 35 DEG C and the humidity of 90% for 6-8 hours every day; the mouse is not placed in the artificial climate box during DSS induction; and (3) DSS treatment: starting from the 21st day, the mice freely drink a DSS sterile aqueous solution for 7 days, then the mice are put into the artificial climate box in the step (2) to freely drink water for 7 days to serve as a recovery period, high-glucose and high-fat feeding is adopted in the period, the period is a molding period, and the period is performed for 3 periods. The built model is similar to ulcerative colitis in traditional Chinese medicine clinical symptoms and signs, good in repeatability and stability and suitable for traditional Chinese medicine screening for treating ulcerative colitis through a traditional Chinese medicine heat clearing and diuresis promoting method.