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A method for cultivating seedlings directly from the germination of *Gynostemma pentaphyllum* seeds in Yunnan.

ActiveCN121464934BSolve the problem of large-scale plantingeasy to operatePlant tissue cultureHorticulture methodsRoot growthCymbidium goeringii
This application discloses a method for directly cultivating seedlings from the germination of *Cymbidium goeringii* seeds in Yunnan. The method includes the following steps: sowing *Cymbidium goeringii* seeds on a modified culture medium, followed by 6 months of constant temperature cultivation at 25℃ to obtain small pseudobulbs suitable for direct transplanting. This method is simple to operate; seeds begin to germinate and turn green 20 days after sowing, begin to grow leaves after 45 days, begin to root after 60 days, and show significant root growth after 90 days. The pseudobulbs also enlarge synchronously. After 6 months, the plants naturally shed their leaves in the tissue culture bottle, and the pseudobulbs can be transplanted to the field for acclimatization and cultivation. This method eliminates the need to change the culture medium, removes the manual transfer step in the tissue culture process, and solves the problems of yellowing, difficult rooting, and high contamination rates after transfer of tissue culture seedlings. Furthermore, the plants naturally shed their leaves in the tissue culture bottle, directly obtaining small pseudobulbs without hardening off, and improving the transplant survival rate, which is beneficial for the large-scale cultivation of *Cymbidium goeringii*.
Owner:KUNMING INST OF BOTANY CHINESE ACAD OF SCI +1