The invention discloses a composite natural material medicinal membrane for treating periodontitis and a manufacturing method thereof, belonging to the field of medicine. The medicinal membrane is prepared from raw materials consisting of, by mass, an extraction concentrate of 16 to 23 parts of dried Rabdosia serra, a water decoction concentrate of 1 to 3 parts of dried honeysuckle flower, a water decoction concentrate of 3 to 5 parts of licorice, a raw juice concentrate of 60 to 70 parts of fresh aloe, 3 to 5 parts of coptis powder, 1 to 3 parts of pearl powder, 1 to 3 parts of scorpion powder, 1 to 3 parts of water-soluble chitosan and 1 to 3 parts of natural glycerin through mixing, heating, stirring for homogenization, membrane making, drying, membrane removal and slicing and packaging. The medicinal membrane uses natural materials or biological preparations, has bacteriostatic, antiviral, anti-inflammatory, detoxifying and pain easing effects and the like, has the excellent characteristics of safety, no stimulation, etc., exerts a special effect on treatment of periodontitis, is applicable to treatment of periodontitis through combination with mechanical scaling and avoids consequences like generation of drug-resistant microflora, adverse reaction of a whole body and the like caused by long-term usage of antibiotics.