5results about How to "The process method is environmentally friendly" patented technology

The invention discloses a cyathea spinulosa polysaccharide compound as well as an extraction method and application thereof. The polysaccharide compound consists of rhamnose, arabinose, galactose and glucuronic acid. The polysaccharide compound is derived from mature leaves of cyathea spinulosa and is extracted by adopting a water extraction and alcohol precipitation method, impurity removal of crude polysaccharide, DEAE sepsis FF ion column separation and Sephacryl S-400HR gel column purification, the method is simple and rapid to operate, water extraction and absolute ethyl alcohol precipitation are mainly adopted in the extraction and separation process, the process method is environment-friendly, the purity of the compound separated by the method is higher than 92%, and the purity of the compound is higher than 99%. The research on the extracted polysaccharide compound shows that the polysaccharide compound has a remarkable hypoglycemic effect, can be used as a raw material for new drug development and pharmacological activity research, and can also be used for food addition or health care product development.

The invention belongs to the technical field of traditional Chinese medicine extraction and separation, and particularly relates to a novel ester alkaloid compound in purslane as well as an extraction and separation method and application thereof. The molecular formula of the new compound is C8H15NO7, and the new compound is named as 5-(1, 2-dihydroxyethylethyl)-3, 4-dihydroxyethylethyran-2-ylglycinate, and the structural formula of the new compound is shown in the description. The invention also provides an extraction and separation method of the novel compound, which sequentially adopts water extraction, macroporous resin adsorption and elution, ODS medium-pressure column preparation, Sephadex LH-20 and high performance liquid chromatograph preparation, and successfully extracts and separates the novel ester alkaloid compound. The structure is determined by mass spectrum, carbon spectrum, hydrogen spectrum and two-dimensional nuclear magnetic spectrum analysis methods. The novel compound has anti-inflammatory and anti-cholinesterase effects, and the novel compound and salts or derivatives thereof can be used as synthetic primers of other compounds and raw materials for new drug development and pharmacological activity research, and can be used for preparing anti-inflammatory and anti-cholinesterase drugs or health care products.

This invention belongs to the field of traditional Chinese medicine chemistry and natural product separation technology, specifically relating to a novel triterpenoid compound and its epimerical form from *Eleutherococcus senticosus*, along with its extraction and separation methods and applications. The new compound is named 22α-Hydroxy-19-epi-chiisanogenin, and its epimerical form is named 22α-Hydroxychiisanogenin. The extraction and separation method provided by this invention employs acid hydrolysis / ethanol reflux extraction, macroporous resin column adsorption, silica gel column chromatography, ODS column chromatography, and high-performance liquid chromatography for separation, purification, and preparation. The compound was successfully extracted and separated. This method involves only five steps, is simple and rapid, and mainly uses conventional reagents such as ethanol, dichloromethane, and methanol. The process is simple and environmentally friendly, and the purity of the compound obtained by this method is high, exceeding 90%. Furthermore, studies have shown that this compound has anti-inflammatory effects. Therefore, the compound of this invention can be used as a lead compound for the synthesis of other compounds, as well as a raw material for new drug development and pharmacological activity research, and can also be used to prepare anti-inflammatory drugs.

The invention belongs to the technical field of traditional Chinese medicine extraction and separation, and particularly relates to a piperidine alkaloid compound extracted, separated and identified from purslane and an extraction and separation method of the piperidine alkaloid compound. The molecular formula of the novel alkaloid compound is C10H14N6O3, and the chemical name of the novel alkaloid compound is 2-(6-amino-9H-purin-9-yl) piperidine-3, 4, 5-triol. The invention further provides an extraction and separation method of the novel alkaloid compound. The extraction and separation method sequentially adopts water decoction extraction, macroporous resin column chromatography, ODS column chromatography, Sephadex LH-20 chromatography and a high performance liquid chromatograph for separation, purification and preparation. The structure of the alkaloid compound is determined as a novel alkaloid compound by mass spectrum, hydrogen spectrum, carbon spectrum and two-dimensional nuclear magnetic spectrum analysis methods. The novel alkaloid compound has anti-inflammatory activity, anti-cholinesterase activity and anti-oxidation activity. The novel alkaloid compound and the salt or derivative thereof can be used as raw materials for drug development and pharmacological activity research.

The invention belongs to the technical field of traditional Chinese medicine extraction and separation, and particularly relates to a pyridine alkaloid compound extracted and separated from purslane as well as an extraction and separation method and application of the pyridine alkaloid compound. The molecular formula of the compound is C7H9NO2, and the chemical name of the compound is 5-ethofyridin-2-ol. The invention further provides an extraction and separation method of the compound. The compound is prepared by sequentially adopting ethanol reflux extraction, ethyl acetate and ethanol reflux extraction in a volume ratio of 2: 1, ODS medium-pressure column chromatography, sephadex LH-20 chromatography and high performance liquid chromatography. The structure is determined by mass spectrum, carbon spectrum, hydrogen spectrum and two-dimensional nuclear magnetic spectrum analysis methods. The compound has anti-inflammatory and anti-oxidation effects, and the compound and salts or derivatives thereof can be used as raw materials for drug development and pharmacological activity research.