This invention belongs to the field of
traditional Chinese medicine chemistry and
natural product separation technology, specifically relating to a novel
triterpenoid compound and its epimerical form from *
Eleutherococcus senticosus*, along with its extraction and separation methods and applications. The new compound is named 22α-Hydroxy-19-epi-chiisanogenin, and its epimerical form is named 22α-Hydroxychiisanogenin. The extraction and
separation method provided by this invention employs
acid hydrolysis /
ethanol reflux extraction,
macroporous resin column adsorption,
silica gel column chromatography, ODS
column chromatography, and high-performance liquid
chromatography for separation, purification, and preparation. The compound was successfully extracted and separated. This method involves only five steps, is simple and rapid, and mainly uses conventional reagents such as
ethanol,
dichloromethane, and
methanol. The process is simple and
environmentally friendly, and the purity of the compound obtained by this method is high, exceeding 90%. Furthermore, studies have shown that this compound has anti-inflammatory effects. Therefore, the compound of this invention can be used as a
lead compound for the synthesis of other compounds, as well as a
raw material for new
drug development and pharmacological activity research, and can also be used to prepare anti-inflammatory drugs.