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Method for determining stainless-steel antibacterial performances by barbital sulfide acid method

A technology of thiobarbituric acid and antibacterial stainless steel, which is applied in the interdisciplinary field, can solve the problems of time-consuming and laborious, inability to identify the antibacterial performance of antibacterial stainless steel, and complex determination procedures of the film-covering method, and achieve the effect of high determination sensitivity and easy operation

Inactive Publication Date: 2009-06-03
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that utilizes thiobarbituric acid method to measure the antibacterial performance of antibacterial stainless steel, solve the methods such as Quinn experiment, antibacterial ring experiment and shaking flask experiment can't identify antibacterial stainless steel antibacterial performance, and cover Membrane method is complex, time-consuming and labor-intensive, and not suitable for investigating the antibacterial properties of a large number of samples. It aims to detect the antibacterial properties of antibacterial stainless steel materials more reliably and conveniently.

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  • Method for determining stainless-steel antibacterial performances by barbital sulfide acid method
  • Method for determining stainless-steel antibacterial performances by barbital sulfide acid method
  • Method for determining stainless-steel antibacterial performances by barbital sulfide acid method

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Embodiment Construction

[0036] The present invention selects Escherichia coli (ATCC25922) as the test strain, and takes the copper-containing ferritic antibacterial stainless steel developed by the Institute of Metal Research, Chinese Academy of Sciences as the test material. The antibacterial performance of copper-containing antibacterial stainless steel is indicated by the content of malondialdehyde after Escherichia coli interacts with various antibacterial samples and control samples. The compositions of antibacterial materials and control samples are shown in Table 1.

[0037] Table 1. Composition (wt%) of antibacterial stainless steel and ordinary stainless steel

[0038] Stainless steel material C Si Mn Ni Mo Cr Nb Cu Other components Ferritic antibacterial stainless steel 0.06 0.70 0.80 - 0.50 16-18 0.40 1.8 trace Ferritic stainless steel 0.06 0.70 0.80 - 0.50 16-18 0.40 - trace

[0039] The indicator bacteria used in the present invention are bacteria such as Escheric...

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Abstract

A method for determining antibacterial ability of antibacterial stainless steel by utilizing TBA means includes determining content of final product-propane aldehyde by TBA means after unsaturated fatty acid in indication bacterial liquid is oxidized, utilizing said content of propane aldehyde to calculate out sterilization rate so as to evaluate out antibacterial ability of antibacterial stainless steel.

Description

Technical field: [0001] The present invention relates to two disciplines of biological science and material science, belongs to interdisciplinary field, and its specific content is to use thiobarbituric acid method (abbreviated TBA method) to measure the oxidized unsaturated fatty acid of bacterial cell membrane after the action of antibacterial stainless steel material Degree, and then calculate the method of antibacterial performance of different antibacterial stainless steel materials. Background technique: [0002] Antibacterial stainless steel is a new type of functional antibacterial material known as having cross-age significance. It has good mechanical and antibacterial properties and has a wide range of applications, especially in areas that emphasize antibacterial, such as food industry, medical equipment, kitchen and bathroom, etc., can be Play an effective and long-lasting role. The development and application of antibacterial stainless steel materials can not o...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33C12Q1/18G06F19/00G06F19/10
Inventor 杨伟超徐慧张颖李慧任瑞霞韩斯琴陈冠雄吕曼祺南黎杨柯
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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