Separating, purifying, culturing and optimizing method for syncyanosen

A symbiotic cyanobacteria, separation and purification technology, which is applied in the field of separation, purification and culture optimization of symbiotic cyanobacteria, can solve problems such as failure to establish a nutrient metabolism model in time.

Inactive Publication Date: 2011-11-16
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the main reason why most symbiotic cyanobacteria are difficult to cultivate successfully in vitro is that the nutrient metabolism mode required for in vitro culture cannot be established in time.

Method used

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  • Separating, purifying, culturing and optimizing method for syncyanosen
  • Separating, purifying, culturing and optimizing method for syncyanosen
  • Separating, purifying, culturing and optimizing method for syncyanosen

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Embodiment

[0015] 1. The separation steps of symbiotic cyanobacteria are as follows:

[0016] (1) Take 0.1g symbiont (in this case, the wild Huangshan pectinium that dries naturally), wash it with tap water and wash it with 0.1% HgCl 2 Surface disinfection to remove bacteria and algae attached to the surface of the stone ear. Soak in sterile water for 1 hour, cut into pieces and transfer to a 10ml glass homogenizer with an appropriate amount of sterile water for grinding and homogenization; filter through a 300-mesh sieve, collect the supernatant and centrifuge at 5000rpm for 1 minute, add 3ml of BG- 11(+N) culture solution resuspends the precipitate; the composition of the BG-11(+N) culture solution containing inorganic nitrogen is as follows: NaNO 3 30.00g / L, K 2 HPO 4 ·3H 2 O 0.788 / L, Na 2 CO 3 0.20g / L, 0.03g / L citric acid, 0.03g / L ferric ammonium citrate, EDTA-Na 2 0.005g / L, CaCl 2 2H 2 O 0.18g / L, MgSO 4 ·7H 2 O 1.5g / L, H 3 BO 3 2.86g / L, MnCl 2 4H 2 O 1.81g / L, CuSO...

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Abstract

The present invention belongs to the fields of the biolobical science and the biotechnology, and relates to a method for abstracting, edulcorating, cultivating and optimizing the symbiotic blue green algae. Separation and purification includes the steps that after the surface sterilization, the immersion, the sterile water cleansing, the glass homogenizer grinding, the centrifuge of filter liquor, the deposition and the re-suspending, a symbiont is cultivated with culture solution containing nitrogen BG-11 (N) in the shaking way in the initiating stage, and cultivated with the culture solution containing non-nitrogen BG-11 (N) in the late stage, at the same time, pure colony is abstracted through marking on the flat plate of the BG-11 (N) solid cultivation medium until being edulcorated. Cultivating and optimizing is provided with the conditions that the intensity of illumination is 160 micromole . m <- 2> . s <-1>, the illumination cycle is 18 h / 6 h, the pH value is 8.4, the temperature is 25 DEG C, and the humidity is 90 percent. The present invention has the advantages that the abstraction and the edulcoration of the symbiotic blue green algae is time saving and labor saving, the vitro nutrition mode of the symbiotic blue green algae can be established rapidly, the very pure symbiotic blue green algae can be obtained, and thus the basis is founded in order to further research the essence of the symbiotic blue green algae and the application of the active metabolic metabolites of the symbiont.

Description

technical field [0001] The invention relates to the separation of biological symbionts, specifically a method for the separation, purification and cultivation optimization of symbiotic cyanobacteria, which is suitable for the establishment of in vitro nutrition and in vitro culture conditions of symbionts in the field of biological science and biotechnology, and is beneficial to symbiosis Applied research on active metabolites in living organisms. Background technique [0002] Symbionts and hosts are in an interdependent and mutually beneficial cooperative relationship. The active substances produced by the metabolism of symbionts play an increasingly important role in medical treatment, health care and modern biopharmaceuticals. As an endosymbiont, it is particularly important to study the metabolism of the symbiont in the host body and the physiological and biochemical characteristics of the symbiont, but it is very difficult to isolate and purify the symbiont and establis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12
Inventor 宋东辉施定基宋海燕时文才张琪
Owner TIANJIN UNIV OF SCI & TECH
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