Antibodies against 25-hydroxyvitamin D

A hydroxyvitamin and antibody technology, applied in anti-hormonal immunoglobulin, anti-animal/human immunoglobulin, peptide, etc., can solve problems such as no reliable method for 25-hydroxyvitamin D

Inactive Publication Date: 2008-09-24
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In particular, there is no mention in the prior art of producing a reliable method for the determination of 25-hydroxyvitamin D

Method used

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  • Antibodies against 25-hydroxyvitamin D
  • Antibodies against 25-hydroxyvitamin D
  • Antibodies against 25-hydroxyvitamin D

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Embodiment Construction

[0019] The present invention relates to a method for producing an antibody capable of resisting 25-hydroxyvitamin D, comprising the following steps:

[0020] a) will contain 25-hydroxyvitamin D 3 or 25-hydroxyvitamin D 2 As a hapten conjugate, immunized into an experimental animal,

[0021] b) separation of serum or plasma from the above-mentioned experimental animals, and

[0022] c) by immunoadsorption to the respective 25-hydroxyvitamin D containing 2 or 25-hydroxyvitamin D 3 On the complementary matrix, so as to purify the antibody in serum or plasma.

[0023] Unless otherwise stated, the term "vitamin D" is understood to include vitamin D according to the following structural formulas I and II 2 and vitamin D 3 Two forms.

[0024] Formula I

[0025]

[0026] Formula II

[0027]

[0028] The positions of vitamin D in structural formulas I and II are indicated according to steroid nomenclature. 25-hydroxyvitamin D refers to the vitamin D metabolite that is h...

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Abstract

The present invention concerns processes for producing antibodies against hydroxyvitamin D, the antibodies produced according to the invention as well as methods for the detection of 25-hydroxyvitamin D using these antibodies.

Description

Background technique [0001] The present invention relates to a method for producing an antibody against 25-hydroxyvitamin D, the antibody produced according to the method of the present invention and a method for detecting 2-hydroxyvitamin D using it. [0002] The term "vitamin" implies that an adequate supply of vitamin D is vital. A deficiency of vitamin D can lead to serious diseases such as rickets or osteoporosis. Although at the beginning of the last century vitamin D was still viewed as a single substance, over the past 30 years the vitamin D system has evolved into a complex and diverse system of vitamin D metabolites. Now there are more than 40 vitamin D metabolites known (Zercrkh, J.E., Ann. Clin. Biochem. 41 (2004) 272-281). [0003] The human body can only produce vitamin D through the action of ultraviolet rays when the sun shines on the skin 3 or calciferol. Vitamin D produced in the skin 3 Binds to the so-called vitamin D-binding protein and is transported ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/26
CPCC07K16/26C07K16/44
Inventor E·休伯J·贝克W·克劳斯A·基里亚特索利斯R·沃格尔N·霍恩
Owner F HOFFMANN LA ROCHE & CO AG
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