Novel biological method for detoxification

A withdrawal and recombinant adenovirus technology, applied in the new biological field of withdrawal syndrome treatment, can solve problems such as side effects, drug addiction, and gradual elimination of the body

Inactive Publication Date: 2008-12-17
俞卫锋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

2. Clinical drugs often require high doses to work, thus bringing significant side effects, such as drug addiction and poisoning
The adenovirus used in this project has been explored and transformed by several generations, and has the characteristics of simple construction process, stability, high titer, convenient amplification, and low side effects. However, like all adenoviruses, its main disadvantage is that it can cause After entering the body, the immune response will be gradually cleared by the body, and basically disappear after five weeks

Method used

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Embodiment Construction

[0015] The present invention will be further described with specific implementation below.

[0016] Specific implementation 1: Production of recombinant adenovirus containing β-endorphin gene expression cassette

[0017] After the DNA fragment of the β-endorphin gene expression cassette (EP) was digested with an appropriate restriction endonuclease, it was ligated with pDC315 cut with the same enzyme, and the ligation product was transformed into Escherichia coli DH5α, and ampicillin (Amp R ) resistance screening positive clones, after correct identification, culture and purification can obtain the pDC315-EP shuttle plasmid, and continue to culture to obtain enough pDC315-EP transfection HEK293 cells.

[0018] The above-mentioned adenovirus shuttle plasmid vector pDC315-EP and backbone plasmid pBHGLoxPΔE1, 3Cre obtained sufficient transfection amount were co-transfected into adenovirus packaging cells such as HEK293. After plaque screening and parallel PCR identification, it ...

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Abstract

The invention belongs to the biological gene engineering field, relating to a production method of recombined adenovirus expressing endogenous opioid peptides (Beta-endorphin) and having curative effect to withdrawal syndrome, and a method for curing the withdrawal syndrome by utilizing the recombined adenovirus. The methods can also be used in the gene therapy of other diseases.

Description

1. Technical field [0001] The invention belongs to the field of biological genetic engineering and provides a new biological method that can be used for the treatment of withdrawal syndrome. 2. Background technology [0002] Currently, the most effective drugs for pain treatment are opioids, but their repeated use is limited due to addiction as one of their side effects. Moreover, as a drug, it is facing a very severe situation. In recent years, the number of drug users in my country has grown rapidly. In 2002, there were 910,000 registered drug users, 1.035 million in 2003, and as many as 1.16 million in 2005. 4 to 8 times that of the drug addict population. Among the total 2863 counties and cities in my country, the number of drug-related counties and cities has grown to 2148. A total of 200 million people worldwide have used drugs at least once. Drug abuse not only destroys the spirit and body of drug addicts, but also causes extremely serious harm to families, society,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/861A61K38/17A61K48/00A61P25/30A61P25/04
Inventor 俞卫锋徐学武
Owner 俞卫锋
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