Method for prolonging retention period of barley blumeria graminis strain

Abstract

The invention discloses a method for prolonging a retention period of a barley blumeria graminis strain, belonging to the technical field of preservation methods of crop pathogenic bacteria. The method comprises the following steps: (1) collecting and propagating monosporous strains; (2) cultivating a test tube seedling of an infected barley variety; (3) inoculating and cultivating the barley test tube seedling; (4) preserving and transplanting the barley blumeria graminis strain, and the like. The method can obviously prolong the retention period of the barley blumeria graminis strain from 8-10 days of a conventional method to 60-70 days and can overcome the defects of easy generation of cross infections, loss of specific pathogenic strains, complicated operation, larger manpower and resource consumption, and the like so as to guarantee the normal development of relevant research works of the pathogenic bacteria of barley powdery mildew. The invention can be popularized and applied to a researching and breeding mechanism of barley diseases.

Description

technical field [0001] The invention relates to the technical field of methods for preserving crop pathogens, in particular to a method for preserving monospore strains of barley powdery mildew. Background technique [0002] Barley is one of the main food crops in the world, and its sown area is second only to wheat, rice and corn, ranking fourth. my country's barley area and total production are second to rice, wheat, corn and millet, ranking fifth. Barley can be used as feed, food, beer and health food. Barley powdery mildew is one of the main diseases of barley. It mostly occurs in humid and semi-humid barley producing areas in the world. It occurs in most barley producing areas in China, mainly in winter barley areas, especially in the southwest and southeast coastal areas. The onset is severe. In recent years, due to the increase in the use of nitrogen fertilizers, the increase in planting density, the single planting variety and the influence of warm winter climate,...

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to propose a method that is easy to operate, time-saving and labor-saving, aiming at the defects of short storage period, time-consuming, labor-intensive and easy to produce cross-infection in the frequent transfer process of the existing barley powdery mildew pathogen in barley live storage. , and can reduce cross-infection between different strains, and can preserve the method of barley powdery mildew strains for a long time, so as to ensure the normal development of related research work on barley powdery mildew pathogens