Method for measuring chlorophyll content of fresh tobacco leaf of flue-cured tobacco based on canopy multi-spectra
A technology of chlorophyll content and determination method, which is applied in the field of determination of chlorophyll content of fresh flue-cured tobacco leaves, can solve the problems of consumption of chemical reagents, damage of tobacco plants, lack of real-time performance, etc.
Active Publication Date: 2010-06-30
ZHENGZHOU TOBACCO RES INST OF CNTC
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The disadvantages are: a. Need to take fresh tobacco leaf samples in the field, which will damage the tobacco plants; b. The measurement cycle is long and not real-time; c. Need to consume chemical reagents
c. Most of the chlorophyll monitoring models reported in the literature are based on grain crops such as wheat and rice. Because flue-cured tobacco is quite different from the above-mentioned crops in terms of plant type, leaf shape, and canopy structure, the chlorophyll content of existing grain crops is relatively large. The prediction model is not suitable for the determination of chlorophyll content in flue-cured tobacco leaves
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[0026] The present invention is further described below in conjunction with embodiment:
[0027] At 11:30 on June 17, 2009, at the National High-quality Tobacco Leaf Production Base in Fangcheng County, Henan Province, the multi-spectrum of the tobacco canopy was measured using the MSR16 multi-spectral radiometer. The field of view of the instrument was 31.1°, and the probe was vertical during the measurement Downward, at a distance of 1.2m from the canopy height, a total of 3 measurements were taken, and the average value of the 3 repetitions was used as the canopy spectral parameter of the tobacco plant. Canopy multi-spectral measurement results show that its ρ550=13.13%, ρ730=41.43%, after calculating its RVI(730,550)=3.31, this canopy RVI(730,550) input into the built model:
[0028] C(chla)=0.250×RVI(730,550) 1.511 , (n=45, R 2 =0.823**) I;
[0029] C(chlb)=0.049×RVI(730,550) 1.841 (n=45, R 2 =0.883**) II;
[0030] The chla=1.53mg / g, chlb=0.44mg / g, chlorophyll tot...
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The invention relates to a method for measuring chlorophyll content of fresh tobacco leaf of flue-cured tobacco based on canopy multi-spectra, which is characterized by comprising the following steps: measuring the canopy spectra of the flue-cured tobacco from a resettling stage to a budding early stage by a multispectral radiometer; establishing vegetation indices; measuring the chlorophyll content of the middle part of the reciprocal fifth leaf of the corresponding plant by a colorimetric method in a laboratory; establishing a model for inverting the chlorophyll content of the leaf by the canopy spectra parameters by a statistical method; measuring the canopy spectra parameters of a sample to be measured; and inputting the canopy spectra parameters into the model, thereby realizing measuring of the chlorophyll content of the flue-cured tobacco leaf. Compared with the prior art, the invention has the following advantages of in-situ measurement, no need of sampling, no injury to tobacco plants and no need of chemical reagents; compared with a hyperspectral radiometer, the invention has larger cost advantage and is more suitable for agricultural production; measurement can be finished only by acquiring data of a 550nm channel and a 730nm channel; and the data processing method and process are simple.
Description
technical field [0001] The method relates to the determination of the chlorophyll content of fresh flue-cured tobacco leaves, in particular to a method for measuring the chlorophyll content of fresh flue-cured tobacco leaves based on canopy multispectral. Background technique [0002] The chlorophyll content of fresh flue-cured tobacco leaves is an important indicator for judging the nitrogen nutrition status of flue-cured tobacco, and has important indicating significance for the decision-making of nitrogen fertilizer management in flue-cured tobacco. [0003] At present, the traditional method for determining the chlorophyll content of flue-cured tobacco fresh tobacco leaves is to take a sample from the field and bring it back to the laboratory, extract it with 95% ethanol or acetone, and measure it by spectrophotometry. The advantage of this method is that it is accurate. The disadvantages are: a. It needs to take fresh tobacco leaf samples in the field, which is harmful...
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IPC IPC(8): G01N21/25
Inventor 王建伟薛超群尹启生周汉平张艳玲宋纪真魏春阳张仕祥梁太波
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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