Recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen gene and preparation method and application thereof

A technology of recombinant baculovirus and PCV-2, which can be used in applications, gene therapy, antiviral agents, etc., can solve the problem of no vaccine and other problems, and achieve the effect of good genetic stability

Inactive Publication Date: 2010-08-04
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

There is currently no vaccine for the control of PCV-2

Method used

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  • Recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen gene and preparation method and application thereof
  • Recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen gene and preparation method and application thereof
  • Recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen gene and preparation method and application thereof

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention and implement it, but the examples given are not intended to limit the present invention.

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Abstract

The invention discloses a recombinant baculovirus expressing porcine circovirus type 2 (PCV-2) immunogen genes and a preparation method and application thereof, in particular relates to a recombinant baculovirus comprising a sequence shown in SEQ ID NO.1 and displaying structural proteins ORF2 of the PCV-2 on the surface. The preparation method comprises steps of utilizing the recombinant baculovirus to inoculate insect cells, culturing for 48-72h, collecting infection cells, carrying out freeze thawing at 40DEG C below zero/37DEG C, centrifuging, taking supernate to test virus PFU and adjusting the titer of the virus to 109PFU/ml to obtain a safe and effective gene engineered subunit vaccine for preventing PCV-2.

Description

technical field [0001] The invention relates to a recombinant baculovirus expressing PCV-2 immunogen gene and its preparation and application. Background technique [0002] Porcine circovirus (PCV) belongs to the genus Circovirus of the family Circoviridae and is a representative species of the genus Circovirus. DNA virus, the average particle diameter of which is 17nm, is the smallest animal virus found in veterinary medicine. [0003] PCV can be divided into two serotypes, PCV-1 and PCV-2. PCV-1 is non-pathogenic to pigs and is a non-pathogenic PCV. PCV-2 is pathogenic to pigs and can cause PMWS (multisystemic wasting syndrome in weaned piglets), also known as PMWS-related PCV. The disease broke out in Canada for the first time in 1991, and there were reports of the disease in many countries and regions in the world. At present, the disease exists in many pig farms in my country, causing serious economic losses to the pig industry in my country. [0004] Hamel et al. fo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/34C12N15/866A61K48/00A61K39/12A61P31/20
Inventor 许信刚童德文张琪
Owner NORTHWEST A & F UNIV
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