Recombinant baculovirus expressing Japanese Encephalitis Virus (JEV) immunogen gene and preparation method and application thereof

Abstract

The invention discloses a recombinant baculovirus expressing Japanese Encephalitis Virus (JEV) immunogen genes and a preparation method and application thereof, particularly relates to a recombinant baculovirus comprising a sequence shown as SEQ ID NO.1 and displaying structural proteins E of the JEV on the surface. The preparation method comprises steps of utilizing the recombinant baculovirus to inoculate Sf9 insect cells, culturing for 48-72h, collecting infection cells, carrying out freeze thawing at 40DEG C below zero / 37DEG C, centrifuging, taking supernate to test virus PFU and adjusting the titer of the virus to 109PFU / ml to obtain a safe and effective gene engineered subunit vaccine for preventing swine JEV.

Description

technical field [0001] The invention relates to a recombinant baculovirus expressing JEV immunogen gene and its preparation and application. Background technique [0002] JE (Japanese encephalitis), also known as Japanese encephalitis (Japanese encephalitis), is an arboviral disease caused by JEV (Japanese encephalitis virus) that is common to humans and animals. Mainly cause reproductive disorders in sows such as miscarriage, premature birth, stillbirth, mummified fetus), and can also cause acute inflammation or infertility in the testes of boars, thereby causing huge economic losses to my country's pig industry. [0003] JEV belongs to the family Flaviviridae, the genus Flavivirus, and is a single-stranded positive-sense RNA virus that does not divide into segments. The genome is about 11Kb long and has a single open reading frame, which can encode, release, and process three structural proteins (envelope protein E, membrane protein M, and capsid protein C) and seven nons...

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