Method for identifying common head cabbage-Chinese cabbage alien addition line
A technology for hetero-additional lines and head cabbage, which is applied in the field of molecular genetics theory application and breeding, can solve the problems of difficulty in accurately identifying different chromosomes in Chinese cabbage, restricting application, etc., and achieves the effects of good communication and improved accuracy.
Inactive Publication Date: 2011-01-26
HEBEI AGRICULTURAL UNIV.
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It can be seen that it is very difficult to accurately identify the different chromosomes of Chinese cabbage under the background of cabbage, which also restricts the application of cabbage-Chinese cabbage heterologous addition lines in breeding practice and genetic theory research
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[0017] According to the technical scheme of the present invention, the identification method of the head cabbage-Chinese cabbage isoaddition line, the specific implementation steps are:
[0018] (1) According to the published genetic map of the Brassica A genome, 207 SSR markers located in the linkage group of the Brassica A genome were selected, and the SSR primer sequences were synthesized by Shanghai Sangon Biotechnology Co., Ltd.
[0019] (2) 10 cabbage varieties (multigenerational self-bred parents: 'Zhonggan'; spring early-maturing varieties: 'Japan Tietou No. The mature variety: 'Jingfeng No.1'; the early-maturing variety for both spring and autumn: 'Zhonggan 17'; the mid-maturing variety for 'Zhonggan 18' in autumn: 'Zhonggan 19'; the late-maturing variety for autumn: 'Wanfeng Cabbage') and 9 Chinese cabbage varieties (multi-generation self-bred parents: '78-13'; very early-maturing varieties: 'Jingchun Baby Cai'; early-maturing varieties: 'Xiaoza 56', 'Jingxiawang'; m...
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The invention discloses a method for identifying a common head cabbage-Chinese cabbage alien addition line, which comprises the following steps of: (1) according to a published genetic map of cabbage, selecting 207 simple sequence repeat (SSR) markers of 10 linkage groups of the Chinese cabbage, wherein primer sequences are synthesized by the Shanghai Sangon Biotech Co., Ltd.; (2) extracting genomic DNAs of the common head cabbage and the Chinese cabbage of different varieties, and performing polymerase chain reaction (PCR) amplification; (3) performing electrophoretic separation on amplification products and performing silver-staining detection; (4) screening the SSR markers, special relative to the common head cabbage, of the linkage groups of the Chinese cabbage; and (5) identifying the common head cabbage-Chinese cabbage alien addition line by utilizing the SSR markers, special relative to the common head cabbage, of the linkage groups of the Chinese cabbage. Chromosomes of the Chinese cabbage and the common head cabbage are small and are similar in form so as to be difficult to accurately identify in a cellular level. The method has the advantages of fast and accurately identifying important genetic analysis materials in a molecular level, such as the Chinese cabbage-common head cabbage alien addition line, an alien substitution line, a translocation line and the like, and laying foundation for improving the genetic background of the common head cabbage by utilizing Chinese cabbage genes.
Description
technical field [0001] The invention relates to a method for identifying head cabbage-Chinese cabbage heterologous lines, and belongs to the technical field of molecular genetics theory application and breeding. Background technique [0002] Heading cabbage (Brassica oleracea var.capitata L., CC) and Chinese cabbage (Brassica campestris L.ssp.pekinensis (Lour) Olsson, AA) are important vegetables in the genus Brassica, which are widely used in vegetable production and people's life in my country. occupies an important position. Establishing head cabbage-Chinese cabbage heteroaddition lines is an important way to carry out genetic theory research and use beneficial genes of Chinese cabbage to improve head cabbage varieties. Because the chromosomes of cabbage and Chinese cabbage are small and the shapes of adjacent chromosomes are very similar, it is difficult to accurately identify each chromosome only by traditional cytological methods. With the development of molecular cyt...
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IPC IPC(8): C12Q1/68
Inventor 申书兴李雪姣顾爱侠陈雪平王彦华轩淑欣李晓峰罗双霞
Owner HEBEI AGRICULTURAL UNIV.