Application of extract of Chinese pulsatilla root to preparation of antischistosomal medicament, method for preparing extract of Chinese pulsatilla root and method for preparing preparation of extract of Chinese pulsatilla root

A technology for anti-schistosomiasis and Pulsatillae, applied in the field of medicine and veterinary medicine, can solve the problem of no anti-schistosomiasis activity found in Pulsatillae medicinal materials

Active Publication Date: 2011-06-01
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

After searching, no literature reports were found that Pulsatilla pulsatillae and its active components have anti-schistosomiasis activity

Method used

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  • Application of extract of Chinese pulsatilla root to preparation of antischistosomal medicament, method for preparing extract of Chinese pulsatilla root and method for preparing preparation of extract of Chinese pulsatilla root
  • Application of extract of Chinese pulsatilla root to preparation of antischistosomal medicament, method for preparing extract of Chinese pulsatilla root and method for preparing preparation of extract of Chinese pulsatilla root
  • Application of extract of Chinese pulsatilla root to preparation of antischistosomal medicament, method for preparing extract of Chinese pulsatilla root and method for preparing preparation of extract of Chinese pulsatilla root

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Embodiment 1

[0023]Embodiment 1, the preparation method of Pulsatillae extract, wherein: the Pulsatillae medicinal material is crushed into coarse powder, soaked in 50% ethanol for 0.5h, 12 times the amount is heated and refluxed for 3 times, each time for 1.5h, filtered, and the filtrates are combined. Concentrate the above filtrate at 70°C to 1.0g raw medicinal materials / mL, refrigerate overnight at 0-4°C, separate the gel, filter, extract the filtrate with n-butanol, recover n-butanol under reduced pressure, dissolve and dilute the residue with water To a concentration of 1.0g raw medicinal materials / mL, filter, and put the filtrate on a D101 macroporous adsorption resin column, the sample volume is 40mg total saponins / g wet resin, the total saponin concentration of the sample solution is 10mg / ml, and the sample flow rate is 1.0ml / min. The pH value of the sample solution was 7.0. After loading the sample, it was eluted with 12 times the amount of distilled water, 30% ethanol, and 60% et...

Embodiment 2

[0024] Embodiment 2, the preparation method of Pulsatillae extract, wherein: grind Pulsatillae medicinal material into coarse powder, soak in 50% ethanol for 0.5h, heat and reflux extraction with 12 times the amount for 3 times, each time for 1.5h, filter, and combine the filtrates. Concentrate the above filtrate at 70°C to 1.0g raw medicinal materials / mL, refrigerate overnight at 0-4°C, separate the gel, filter, extract the filtrate with n-butanol, recover n-butanol under reduced pressure, dissolve and dilute the residue with water To a concentration of 1.0g raw medicinal materials / mL, filter, put the filtrate on the AB-8 macroporous adsorption resin column, the loading amount is 40mg total saponins / g wet resin, the total saponin concentration of the loading sample solution is 10mg / ml, and the loading flow rate is 1.0 ml / min, the pH value of the sample solution is 7.0, after loading the sample is eluted with 12 times the amount of distilled water, 20% ethanol, and 60% ethanol....

Embodiment 3

[0025] Embodiment 3: the preparation method of the Pulsatillae extract, wherein: the Pulsatillae medicinal material is crushed into a coarse powder, soaked in 50% ethanol for 0.5h, 12 times the amount is heated and refluxed for 3 times, each time for 1.5h, filtered, and the filtrates are combined. The above-mentioned filtrate was concentrated under reduced pressure at 70°C to 1.0g raw medicinal materials / mL, refrigerated overnight at 0-4°C, separated from the gel, filtered, and the filtrate was applied to a HPD-450 macroporous adsorption resin column, and the loading amount was 40mg total saponins / g wet Resin, the total saponin concentration of the sample solution is 10mg / ml, the sample flow rate is 1.0ml / min, and the pH value of the sample solution is 7.0. After the sample is loaded, it is eluted with 12 times the amount of distilled water, 20% ethanol, and 60% ethanol. The flow rate was 1.5ml / min, and the 60% ethanol eluate was collected. The above eluate was concentrated un...

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Abstract

The invention provides application of an extract of Chinese pulsatilla root to preparation of an antischistosomal medicament. The invention also provides a method for preparing the extract of Chinese pulsatilla root and a method for preparing a preparation of the extract of Chinese pulsatilla root. The method for preparing the extract of Chinese pulsatilla root comprises the following steps of: grinding Chinese pulsatilla root into coarse powder; soaking the coarse powder for 0.5 to 2 hours by 10 to 95 percent of ethanol; carrying out heating reflux extraction on the obtained product for 2 to3 times for 1 to 3 hours each time by using water of which the volume is 6 to 12 times of that of the obtained product; filtering the product and merging filtrate; and refining to obtain the extract of Chinese pulsatilla root by macroporous absorption resin columns on the filtrate. The extract of Chinese pulsatilla root has obvious antischistosomal activity and can be used for preventing and treating schistosomiasises of people or other mammals.

Description

technical field [0001] The invention belongs to the technical field of medicine and veterinary medicine, and relates to the application of Pulsatilla pulsatillae in the preparation of anti-schistosomiasis drugs. The invention also provides a preparation method of Pulsatilla pulsatillae extract and a preparation method thereof. The effective component has significant anti-schistosomiasis activity, It can be used for the prevention and treatment of schistosomiasis in humans or other mammals. Background technique [0002] Pulsatilla medicinal material is taken from the dried root of Pulsatilla chinensis (Bunge) Regel, a plant of the genus Pulsatilla in the family Ranunculaceae, which is bitter in taste and cold in nature. Return stomach, large intestine meridian, tool heat-clearing and toxic substances removing, the merit of cooling blood and stopping dysentery. For pyretotoxic blood dysentery, pruritus vulvae leukorrhagia, amoebic dysentery. According to literature reports, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P33/12A61K125/00
CPCY02A50/30
Inventor 杨世林李孝贤简晖许琼明罗晓健李笑然夏超明刘艳丽饶毅冯育林范玫玫龚建平
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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