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Preparation method of poultry specific transfer factors

A transfer factor and specific technology, applied in the field of medicine, can solve the problems of non-specific disease and unstable treatment effect, and achieve the effect of improving immunity

Inactive Publication Date: 2013-07-24
TIANJIN ASIAN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of transfer factors prepared in the prior art that are not specific to diseases and have unstable therapeutic effects by providing a method for preparing poultry-specific transfer factors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method for specific transfer factor for poultry, the specific steps are:

[0020] (1) Aseptically take the anticoagulated blood of pigs, and prepare a monolayer of lymphocytes with peripheral blood, the steps are:

[0021] Take 25ml of pig whole blood via sterile vein, add 0.8% heparin solution 0.3ml for anticoagulation, let it stand for 45 minutes, use a pipette with a rubber ball to absorb all the plasma above the red blood cell layer, especially the white blood cell layer immediately above the red blood cell layer. Aspirated, divided into sterilized centrifuge tubes, washed repeatedly 2 to 3 times with PBS (pH 7.2) solution, centrifuged at 800 to 1200 rpm, and then cultured white blood cells with 40 ml of hydrolyzed milk protein containing 30% calf serum. After uniform mixing, it is divided into 100 ml culture square bottles or brick bottles, the bottle stopper is tightly closed, and cultured in a 37°C incubator or a brick bottle machine. After 2 to 3 d...

Embodiment 2

[0029] A preparation method for specific transfer factor for poultry, the specific steps are:

[0030] (1) Aseptically take the spleen of the experimental pig, and use the spleen to prepare a monolayer of lymphocytes, the steps are:

[0031] A. Processing pig spleen

[0032] Take fresh pig spleen and place it in a sterile container, add PBS (pH 7.2) solution to soak for half an hour or immerse in 1% bromogeramine solution by mass percentage, take out and disinfect the surface of the spleen with alcohol, remove it with dissecting scissors and tweezers Spleen fat and capsule were washed once with PBS (pH 7.2), then cut the spleen into small pieces and transferred to a plate, and finally cut the spleen into 1mm3 small pieces with ophthalmic scissors, and then washed with PBS (pH 7.2) for 2 -3 times to remove blood cells, pigment substances and cells damaged mechanically during the shredding process;

[0033] B. Digest and disperse tissue blocks

[0034] Suck off the PBS (pH 7....

Embodiment 3

[0051] A preparation method for specific transfer factor for poultry, the specific steps are:

[0052] (1) Take fresh pig spleen and place it in a sterile glass container, add PBS (pH 7.2) solution to soak for half an hour or immerse in brogeramine solution with a mass percent concentration of 1%, take out the surface of the spleen with alcohol to disinfect it, and dissect it Scissors and tweezers to remove the spleen fat and capsule, washed once with PBS (pH 7.2), then cut the spleen into small pieces and put them in a plate, finally cut the spleen into 1mm3 small pieces with ophthalmic scissors, and then washed them with PBS (pH 7.2) ) liquid washing 2-3 times;

[0053] (2) Suck off the PBS (pH7.2) solution washed in the previous step, move the shredded tissue pieces into an Erlenmeyer flask, and add 0.25% trypsin solution (pH 7.6~ 7.8), placed in a 37°C water bath for 20-40 minutes for digestion. Shake the Erlenmeyer flask every 10 minutes to disperse the tissue pieces and ...

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Abstract

The invention provides a preparation method of poultry specific transfer factors, relating to the field of medicines, in particular to a preparation method of a poultry specific transfer factor. The preparation method comprises the steps of: with pig spleen or peripheral blood as a raw material, preparing a lymphocyte monolayer, culturing lymphocyte in vitro, then inducing and culturing by using polyinosinic polycytidylic acid and infectious bronchitis virus, and producing a large quantity of infectious bronchitis virus resistant specific transfer factors in vitro. The infectious bronchitis virus resistant specific transfer factors prepared in vitro by using the method are used for treating the infectious bronchitis and improving poultry immunity.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of specific transfer factor for poultry. Background technique [0002] Chicken infectious bronchitis disease is a common infectious disease in chickens. It is a highly contagious disease caused by infectious bronchitis virus and has a high mortality rate. There is currently no effective drug treatment for chicken infectious bronchitis disease. Control methods should be based on epidemic prevention, quarantine and disinfection. [0003] Transfer factor is a low-molecular-weight polypeptide and nucleic acid complex extracted from lymphocytes, which can specifically or non-specifically improve the immune function of the body and transmit immune information. It is known as a cellular immune activator. It has no species specificity, can be transmitted between species, and animal sources can be used in humans. Transfer factor is a defensive peptide active substance produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/28A61K35/14A61P31/14A61P11/00A61K35/26
Inventor 王文彪王丽莉
Owner TIANJIN ASIAN BIOENG
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