Clip based sampling of seed for the removal of specific seed tissue or structures for seed analysis

A seed and genetic technology, applied in the direction of analyzing materials, seed and rhizome treatment, sampling devices, etc., to achieve the effect of reducing the amount of site space

Inactive Publication Date: 2011-08-17
PIONEER HI BRED INT INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, automation introduces not only cost and complexity but also real outcomes

Method used

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  • Clip based sampling of seed for the removal of specific seed tissue or structures for seed analysis
  • Clip based sampling of seed for the removal of specific seed tissue or structures for seed analysis
  • Clip based sampling of seed for the removal of specific seed tissue or structures for seed analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0123] 2. Example 2—cut the sample individually with a cigar-type multi-blade cutter ( Figure 2A )

[0124] a) device ( Figure 2A )

[0125] Figure 2A A single seed sample tool 300 is shown hand or bench mounted. The funnel-shaped housing or body 302 holds the curved cutting blades 310 of a conventional commercially available cigar cutter 309 (eg, three tangentially moving blades with scissor-type handles), which will be referred to as the cutting plane. Some cigar cutters have more than three blades.

[0126] Such cigar cutters are commercially available. Examples are the XiMTX Multi-Tool available from Xikor of Kansas City, MO 64102 and the Model 8173B Scissor Cigar Cutter available from Cubanoz.biz of Miami Beach, FL 33119.

[0127] A hinged cover 306 attached around the body 302 has at its center a rubber or flexible gasket 308 sized to accommodate a single corn seed 3 when the seed 3 is pushed into the center of the gasket 308 . The geometry of the cover 306 and...

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PUM

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Abstract

Apparatus, methods, and systems for useful sampling of seed, wherein viability is maintained, are disclosed. Seed from one generation in a plant advancement experiment is individually sampled by removal and collection of tissue from the seed using a hand-held and manually operated tool having one or more cutting edges. The tissue is then processed to derive one or more biochemical, genetic, or phenotypic characteristic of the seed before a decision is made whether to utilize that seed further in a plant advancement experiment or other plant research and development. In some embodiments of the method, the sampling is controlled to remove a useful amount of tissue for analytical purposes without significant effect on viability potential of the sampled seed. In some embodiments, the sampling is controlled to deter contamination of the sample. In some embodiments, the seed is held in pre-determined orientation to facilitate efficient and accurate sampling.

Description

[0001] This application is a divisional application with the original application number 200780049369.X, the filing date being November 13, 2007, and the title of the invention being "Seed Sampling Based on Pruning to Remove Specific Seed Tissue or Structure for Seed Analysis". 【Technical field】 [0002] The present invention relates to an efficient method for obtaining tissue samples from individual seeds. 【Background technique】 [0003] It is common practice in plant breeding or plant evolution experiments to grow plants from seeds of known origin. Seeds are planted in test plots, growth chambers, greenhouses, or other growing environments where they are either cross-pollinated with plants of other known origin or self-pollinated. The seeds produced are progeny from both parent plants or self-pollinated plants, and the seeds are harvested, processed and planted to continue the plant breeding cycle. To aid in the cultivation or to facilitate the selection process, specific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/04G01N1/28A01C1/00
CPCG01N1/28A01C1/025B26B17/00G01N35/00A24F13/24G01N35/0098G01N2033/0077A01G3/00G01N1/04G01N2035/00564B26B13/00
Inventor S・M・贝克J・科普
Owner PIONEER HI BRED INT INC
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