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Isothermal chain multiple detection card of pathogen nucleic acid

A pathogen and detection card technology, which is applied in the field of temperature chain multiple detection cards, can solve the problems of operational technology responsibility, special equipment, and long time consumption, so as to achieve clear and variable results, prevent amplification cross-contamination, and prevent cross-contamination.

Active Publication Date: 2013-06-12
武汉中科志康生物科技有限公司
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Problems solved by technology

[0003] The object of the present invention is to provide a kind of isothermal chain multiple detection card of pathogenic nucleic acid according to existing deficiencies in the pathogen detection method, this detection card has combined the nucleic acid isothermal chain displacement method of multiple detection pathogen and nano colloidal gold technology, overcomes The disadvantages of traditional detection methods such as technical responsibility, time-consuming, and special instruments are required

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Embodiment Construction

[0020] The following examples will further explain the present invention, but the examples do not limit the present invention in any form.

[0021] The method of the present invention uses the isothermal strand replacement technology for multiple detection of pathogen nucleic acid to prepare:

[0022] 1. The hairpin structure sequence for hepatitis B virus detection is shown in SEQ ID NO: 1, the extension primer is shown in SEQ ID NO: 2, and the nucleotide sequence corresponding to the T line for detection is shown in SEQ ID NO: 3.

[0023] The hairpin structure sequence for hepatitis C virus detection is shown in SEQ ID NO: 4, the extension primer is shown in SEQ ID NO: 5, and the nucleotide sequence corresponding to the T line for detection is shown in SEQ ID NO: 6.

[0024] The human immunodeficiency virus detection hairpin structure sequence is shown in SEQ ID NO: 7, the extension primer is shown in SEQ ID NO: 8, and the nucleotide sequence corresponding to the T line for detection...

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Abstract

The invention discloses an isothermal chain multiple displacement detection card of pathogen nucleic acid. The isothermal chain multiple displacement detection card of the pathogen nucleic acid utilizes the detection card which is prepared through a nucleic acid isothermal chain displacement method and a colloidal gold detection technology to carry out multiple detection on HBV-DNA, HCV-RNA, HIV-RNA, and TP-DNA. The isothermal chain multiple displacement detection card of the pathogen nucleic acid has the characteristics of simple and rapid operation, high sensitivity, and no need of professional equipment. The pathogen nucleic acid to be detected is not amplified in the detection process, so the detection card has the advantages of preventing amplified matter cross contamination in laboratories and preventing false positivity, can be widely used in high sensitivity pathogen nucleic acid detection in the fields of clinical detection, inspection and quarantine, infectious disease control, biological technology and the like, and has a wide application prospect.

Description

Technical field [0001] The invention relates to the technical field of biological detection, in particular to an isothermal chain multiple detection card for pathogen nucleic acid. Background technique [0002] Traditional pathogen detection methods include the detection of pathogen antibodies based on serology, and molecular detection methods, such as the detection of pathogen nucleic acid. The detection of pathogen antibodies by enzyme-linked immunoassay has certain advantages, such as good specificity and high reproducibility, but this method cannot be used to diagnose active and chronic infections. Since the establishment of the nucleic acid detection method, this shortcoming has been resolved. The nucleic acid detection method is to directly detect the ribonucleic acid or deoxyribonucleic acid of the pathogen. Both qualitative and quantitative methods have certain advantages. Although nucleic acid detection methods have the advantages of high sensitivity and high specificit...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 何秀华黄菲
Owner 武汉中科志康生物科技有限公司
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