Isothermal chain multiple detection card of pathogen nucleic acid
A multiple detection and pathogen technology, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of time-consuming, responsible operation technology, special equipment, etc., to prevent cross-contamination, clear and variable results, The effect of preventing amplification cross-contamination
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[0020] The following examples will further explain the present invention, but the examples do not limit the present invention in any form.
[0021] The method of the present invention uses the isothermal strand replacement technology for multiple detection of pathogen nucleic acid to prepare:
[0022] 1. The hairpin structure sequence for hepatitis B virus detection is shown in SEQ ID NO: 1, the extension primer is shown in SEQ ID NO: 2, and the nucleotide sequence corresponding to the T line for detection is shown in SEQ ID NO: 3.
[0023] The hairpin structure sequence for hepatitis C virus detection is shown in SEQ ID NO: 4, the extension primer is shown in SEQ ID NO: 5, and the nucleotide sequence corresponding to the T line for detection is shown in SEQ ID NO: 6.
[0024] The human immunodeficiency virus detection hairpin structure sequence is shown in SEQ ID NO: 7, the extension primer is shown in SEQ ID NO: 8, and the nucleotide sequence corresponding to the T line for detection...
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