Markers for endometrial cancer

An endometrial cancer and marker technology, which can be used in the analysis of biomaterials, biochemical equipment and methods, and the determination/inspection of microorganisms, and can solve problems such as changes in endometrial thickness.

Active Publication Date: 2012-07-04
GEADIC BIOTEC AIE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A potential problem with the use of TVS in premenopausal and perimenopausal women is that endometrial thickness varies with the phase of the menstrual cycle

Method used

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  • Markers for endometrial cancer
  • Markers for endometrial cancer
  • Markers for endometrial cancer

Examples

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Comparison scheme
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example

[0518] The following examples are included herein to demonstrate the preferred embodiments of the invention. It should be appreciated by those of skill in the art that, following the representative techniques used by the inventors, the techniques disclosed in the examples have functioned well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, after reading the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

example 1

[0519] Example 1: Identification of Endometrial Cancer Biomarkers

[0520] In order to identify biomarkers for predicting and / or diagnosing endometrial cancer, 56 primary endometrial tumors at various stages of differentiation were compared with 10 normal (i.e. The gene expression levels of endometrial cancer tissues were compared. This technique allows us to examine the expression of this whole genome in specific types of cells, tissues, organs or, in this case, differential gene expression between endometrial cancer and healthy endometrial tissue. A microarray chip contains small DNA sequences arranged in a regular pattern with probes for often thousands of genes at specific locations.

[0521] The amount of a particular mRNA in a sample can be assessed by its hybridization signal on the array.

[0522] Sample description

[0523]Tumor samples were obtained from patients who had undergone surgery, and control tissue was obtained from non-diseased areas of endometrial ti...

example 2

[0548] Example 2: Taking a Uterine Fluid Sample

[0549] Endometrial aspirates were collected from all patients with the aid of an endometrial sampling device (Cornier pipelle) after full informed consent. This aspirate (uterine fluid) was immediately transferred to an eppendorf tube containing 500 microliters of RNA protection solution (RNAlater, Ambion). The sample was centrifuged and a pellet containing a representative population of cells from the uterine cavity was further processed for RNA extraction (Qiagen). After quality testing (Bioanalyzer), gene expression was analyzed for selected endometrial cancer markers by Taqman technology.

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Abstract

The invention relates to the surprising finding that biomarkers corresponding to ACAA1, AP1M2, CGN, DDR1, EPS8L2, FASTKD1, GMIP, IKBKE, P2RX4, P4HB, PHKG2, PPFIBP2, PPP1 R16A, RASSF7, RNF183, SIRT6, TJP3, EFEMP2, S0CS2, and DCN are differentially expressed in control samples as compared to samples from patients having endometrial cancer and are therefore useful for detecting endometrial cancer. In particular these biomarkers having excellent sensitivity, specificity, and / or the ability to separate affected from non affected individuals. Furthermore, the inventors found that the differential expression of these biomarkers in primary endometrial cancer tumor tissue is correlated to their expression level in uterine fluid samples as compared to control values.; Thus these biomarkers are robust in that they are found to be differentially expressed in several different types of samples from affected individuals.

Description

technical field [0001] The invention relates to the detection, diagnosis and prognosis of uterine cancer. The present invention relates to the surprising discovery that the genes corresponding to ACAA1, AP1M2, CGN, DDR1, EPS8L2, FASTKD1, GMIP, IKBKE, P2RX4, P4HB, PHKG2, PPFIBP2, PPP1R16A, RASSF7, RNF183, SIRT6, TJP3, EFEMP2, SOCS2 and DCN The biomarkers are differentially expressed in control samples versus endometrial cancer patient samples and are thus useful for detecting endometrial cancer. In particular, these biomarkers have excellent sensitivity, specificity and / or ability to distinguish diseased individuals from non-diseased individuals. Furthermore, the inventors found that the differential expression of these biomarkers in primary endometrial cancer tumor tissues correlated with their expression levels in uterine fluid samples compared to control values. These biomarkers are thus robust because they were found to be differentially expressed in several different typ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q2600/112C12Q2600/156C12Q2600/16G01N33/57442C12Q1/6886G01N2800/60C07K16/3069C07K2317/34C12Q1/6837C12Q2600/158
Inventor 米格尔·阿巴尔波萨达安德烈亚斯·多尔安东尼奥·吉尔莫雷诺塔玛拉·马埃斯克里斯帝娜·佩雷斯豪梅·雷维托斯普伊赫简尔伊丽莎贝·罗塞尔
Owner GEADIC BIOTEC AIE
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