Observation cell arrangement

A technology for observing instruments and cells, which is applied in the field of drug identification, can solve the problems of high cost, difficulty, and difficult culture medium, and achieve the effect of easy detection

Inactive Publication Date: 2012-08-15
UNIVERSITY OF NOTTINGHAM
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Problems solved by technology

There were also inherent problems in earlier techniques for routine observation of cells in microscope instruments. For example, the use of a single gravity and pressure system meant that it was difficult to maintain the culture medium at the desired temperature. The applied pressure depended on the volume of culture medium, it is difficult to connect such a system to a pressure pump
Other technologies utilize a syringe pump system, which is also difficult to achieve by relying on one pump to control all independent containers, that is, all injection gas cylinders, each of which maintains the same temperature in each container depends on the injection gas cylinder, and also uses the injection gas cylinder it will cost a lot

Method used

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Embodiment Construction

[0062] As mentioned above, in fluid perfusion systems, it is difficult to make reliable observations due to the turbulence of the fluid in the flow. This problem is especially acute in setups including confocal microscopes. Potential perturbations create enormous difficulties due to the fine focus present in confocal microscopy systems. It is understood that the focal plane of a confocal microscope system will be limited to 1 μm (or less), such that pressure pulses and other changes can change the picture temporarily or over time, which can make the observations necessary for the correct analysis of cultured cell systems difficult. I don't know. Ideally, continuous laminar flow across cultured cell systems should be provided. Also, if a fluorescence detector or other measurement equipment is used in the instrument, the disturbance of the fluid will make the measurement / observation result inaccurate, and the sample to be observed will move out of the field of view, or focus, ...

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Abstract

An observation cell arrangement for flow perfusion of a sample to be examined, the arrangement comprising a flow cell (21) having a cavity therein to receive the sample, the flow cell (21) arranged to receive a flow of fluid through the cavity that is directed over the sample from a cavity inlet (22) to a cavity outlet (23), the cavity inlet (22) associated with a fluid supply line, and a first flow supply path (24) connected to the fluid supply line via a valve (39), the first flow supply path (24) adapted to receive pressure from a pressure source comprising a pressure reservoir (29) to drive fluid flow through the cavity at a desired flow rate.

Description

technical field [0001] The present invention relates to a cell observation apparatus, in particular to a cell observation apparatus in which nutrient fluid is perfused to maintain cultured cells during observation. Furthermore, it relates to a method of observation under fluid perfusion and a method of identifying drugs. Background technique [0002] Perfusion systems are used for a range of viable cells that require a constant flow of nutrient medium. [0003] Confocal microscopy is a technique used to increase microscopic contrast and / or reconstruct three-dimensional images by effectively eliminating out-of-focus light in samples thicker than the theoretical focal plane. The technique is popular in the life sciences where changes in cells need to be observed. It is clear that in a conventional microscope, a fluorescence microscope with a wide field of view, the entire sample is flooded with bright light from the light source. In this case, all parts of the sample in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N21/05G01N21/11
CPCG01N15/1463G01N21/6458G01N35/08G01N35/1095
Inventor 布莱恩·莫里斯提姆·塞尔夫斯蒂芬·约翰·希尔
Owner UNIVERSITY OF NOTTINGHAM
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