Method for improving carbon fixing efficiency of non-photosynthesizing microorganisms by utilizing hybrid electron donor

A technology of non-photosynthetic microorganisms and electron donors, applied in the direction of adding compounds to stimulate growth, bacteria, etc., can solve the problems of limited availability of energy materials, limited types of energy materials, limited amount of energy materials, etc., to promote carbon sequestration Efficiency, carbon sequestration efficiency synergy, operability effect

Inactive Publication Date: 2012-09-12
TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the carbon fixation efficiency of these flora is lower than that of photosynthetic microorganisms. The main reason is that these flora can use limited types of energy substances, limited energy substances, and limited availability of energy substances. If a mixed electron donor system is used, then These problems can be effectively solved, which will help to significantly improve its carbon sequestration efficiency, and further enhance the potential economic and social benefits of bacteria in practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of medium for cultivating autotrophic microorganisms

[0033] The medium formula is as follows (g / L): KH 2 PO 4 (1.0);K 2 HPO 4 (2.0);MgSO 4 ·7H 2 O(0.2); NaCl(20) and CaCl 2 (0.01); (NH 4 ) 2 SO 4 (0.038mol / L); FeSO 4 ·7H 2 O (0.0036mmol / L); Add 2mL of trace element solution to each liter of the above medium.

[0034] The trace element solution is selected from the group containing Na 2 MoO 4 2H 2 O(1.68);H 3 BO 3 (0.4);ZnSO 4 ·7H 2 O(1.0);MnSO 4 ·5H 2 O(1.0);CuSO 4 ·5H 2 O(7.0);CoCl 2 ·6H 2 O(1.0) and NiSO 4 ·7H 2 O (1.0), the concentration is in mg / L;

[0035] (2) NaNO used as electron donor after separate preparation 2 , Na 2 S 2 o 3 and Na 2 Concentrates of S, the concentration of which is 80mg / mL;

[0036] (3) Select several kinds of electron donor concentrates obtained in step (2), according to 10.4g / L NaNO 2 ,10.7g / L Na 2 S 2 o 3 and 9.8g / LNa 2 The consumption of S adds the electron donor concentrated solution...

Embodiment 2

[0039] (1) Prepare a culture medium for cultivating autotrophic microorganisms. The medium formula is as follows (g / L): KH 2 PO 4 (1.0);K 2 HPO 4 (2.0);MgSO 4 ·7H 2 O(0.2); NaCl(20) and CaCl 2 (0.01); (NH 4 ) 2 SO 4 (0.038mol / L); FeSO 4 ·7H 2 O (0.0036mmol / L) Add 2mL of trace element solution to each liter of the above medium.

[0040] The trace element solution is selected from the group containing Na 2 MoO 4 2H 2 O(1.68);H 3 BO 3 (0.4);ZnSO 4 ·7H 2 O(1.0);MnSO 4 ·5H 2 O(1.0);CuSO 4 ·5H 2 O(7.0);CoCl 2 ·6H 2 O(1.0) and NiSO 4 ·7H 2 O (1.0), the concentration is in mg / L;

[0041] (2) NaNO used as electron donor after separate preparation 2 , Na 2 S 2 o 3 and Na 2 Concentrates of S, the concentration of which is 160mg / mL;

[0042] (3) Select several kinds of electron donor concentrates obtained in step (2), according to 0.01g / L MnSO 4 ·5H 2 O,10.4g / LNaNO 2 ,10.7g / L Na 2 S 2 o 3 and 9.8g / L Na 2 Amount of S Add the electron donor concentrate...

Embodiment 3

[0046] (1) Prepare a medium for cultivating autotrophic microorganisms. The medium formula is as follows (g / L): KH 2 PO 4 (1.0);K 2 HPO 4 (2.0);MgSO 4 ·7H 2 O(0.2); NaCl(20) and CaCl 2 (0.01); NH 4 Cl (0.019mol / L); FeCl 2 (0.00144mmol / L); Add 2mL of trace element solution in every liter of the above medium. The trace element solution is selected from the group containing Na 2 MoO 4 2H 2 O(1.68);H 3 BO 3 (0.4);ZnSO 4 ·7H 2 O(1.0);MnSO 4 ·5H 2 O(1.0);CuSO 4 ·5H 2 O(7.0);CoCl 2 ·6H 2 O(1.0); and NiSO 4 ·7H 2 O(1.0), the concentration is in mg / L.

[0047] (2) As the electron donor after separate preparation, NaNO 2 , Na 2 S 2 o 3 and Na 2 Concentrate of S with a concentration of 200 mg / mL;

[0048] (3) Select several kinds of electron donor concentrates obtained in step (2), according to 5.0g / L NaNO 2 or 1.7g / LNa 2 S 2 o 3 or 3.3g / L Na 2 The amount of any electron donor in S is added to the culture medium obtained in step (1) by adding the electro...

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Abstract

The invention relates to the field of microorganism fixing of CO2, and in particular relates to a method for improving carbon fixing efficiency of non-photosynthesizing microorganisms in an oxygen deficient condition. The method comprises the following steps: (1) preparing a culture medium for culturing autotrophic microorganisms, and adding trace element solution in the culture medium; (2) preparing an electron donor concentrate; (3) adding the electron donor concentrate prepared in the step (2) into the trace element containing culture solution prepared in the step (1); and (4) adding hybrid carbon-fixing strains from oceans into the culture medium prepared in the step (3). According to the method, the carbon-fixing efficiency of the non-photosynthesizing microorganisms can be effectively increased. The method has the advantages of simple process, strong operability and certain economic benefit.

Description

technical field [0001] The invention belongs to microbial fixation of CO 2 The field relates to a method for improving the carbon fixation efficiency of non-photosynthetic microorganisms under anoxic conditions. Background technique [0002] CO 2 The global warming caused by the "greenhouse effect" is a major environmental problem facing the world. According to the 4th assessment report of IPCC, in the most recent ten-year period (1995-2004), CO 2 The rate of increase in equivalent emissions (920 million tons of CO per year 2 equivalent) compared with the emission rate of the previous decade (1970-1994) (430 million tons of CO per year 2 Equivalent) is much higher. China's "Twelfth Five-Year Plan Proposal Draft" shows that my country will CO 2 Emission reduction is placed in a very important position. At the Cancun Conference held in late 2010, China pledged that by 2020, the unit of gross domestic product (GDP) CO 2 Emissions will drop by 40%-45% compared to 2005. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20
Inventor 胡佳俊王磊张士萍胡煜席雪飞陈金海李凡
Owner TONGJI UNIV
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