Method for improving carbon fixing efficiency of non-photosynthesizing microorganisms by utilizing hybrid electron donor
A technology of non-photosynthetic microorganisms and electron donors, applied in the direction of adding compounds to stimulate growth, bacteria, etc., can solve the problems of limited availability of energy materials, limited types of energy materials, limited amount of energy materials, etc., to promote carbon sequestration Efficiency, carbon sequestration efficiency synergy, operability effect
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Embodiment 1
[0032] (1) Preparation of medium for cultivating autotrophic microorganisms
[0033] The medium formula is as follows (g / L): KH 2 PO 4 (1.0);K 2 HPO 4 (2.0);MgSO 4 ·7H 2 O(0.2); NaCl(20) and CaCl 2 (0.01); (NH 4 ) 2 SO 4 (0.038mol / L); FeSO 4 ·7H 2 O (0.0036mmol / L); Add 2mL of trace element solution to each liter of the above medium.
[0034] The trace element solution is selected from the group containing Na 2 MoO 4 2H 2 O(1.68);H 3 BO 3 (0.4);ZnSO 4 ·7H 2 O(1.0);MnSO 4 ·5H 2 O(1.0);CuSO 4 ·5H 2 O(7.0);CoCl 2 ·6H 2 O(1.0) and NiSO 4 ·7H 2 O (1.0), the concentration is in mg / L;
[0035] (2) NaNO used as electron donor after separate preparation 2 , Na 2 S 2 o 3 and Na 2 Concentrates of S, the concentration of which is 80mg / mL;
[0036] (3) Select several kinds of electron donor concentrates obtained in step (2), according to 10.4g / L NaNO 2 ,10.7g / L Na 2 S 2 o 3 and 9.8g / LNa 2 The consumption of S adds the electron donor concentrated solution...
Embodiment 2
[0039] (1) Prepare a culture medium for cultivating autotrophic microorganisms. The medium formula is as follows (g / L): KH 2 PO 4 (1.0);K 2 HPO 4 (2.0);MgSO 4 ·7H 2 O(0.2); NaCl(20) and CaCl 2 (0.01); (NH 4 ) 2 SO 4 (0.038mol / L); FeSO 4 ·7H 2 O (0.0036mmol / L) Add 2mL of trace element solution to each liter of the above medium.
[0040] The trace element solution is selected from the group containing Na 2 MoO 4 2H 2 O(1.68);H 3 BO 3 (0.4);ZnSO 4 ·7H 2 O(1.0);MnSO 4 ·5H 2 O(1.0);CuSO 4 ·5H 2 O(7.0);CoCl 2 ·6H 2 O(1.0) and NiSO 4 ·7H 2 O (1.0), the concentration is in mg / L;
[0041] (2) NaNO used as electron donor after separate preparation 2 , Na 2 S 2 o 3 and Na 2 Concentrates of S, the concentration of which is 160mg / mL;
[0042] (3) Select several kinds of electron donor concentrates obtained in step (2), according to 0.01g / L MnSO 4 ·5H 2 O,10.4g / LNaNO 2 ,10.7g / L Na 2 S 2 o 3 and 9.8g / L Na 2 Amount of S Add the electron donor concentrate...
Embodiment 3
[0046] (1) Prepare a medium for cultivating autotrophic microorganisms. The medium formula is as follows (g / L): KH 2 PO 4 (1.0);K 2 HPO 4 (2.0);MgSO 4 ·7H 2 O(0.2); NaCl(20) and CaCl 2 (0.01); NH 4 Cl (0.019mol / L); FeCl 2 (0.00144mmol / L); Add 2mL of trace element solution in every liter of the above medium. The trace element solution is selected from the group containing Na 2 MoO 4 2H 2 O(1.68);H 3 BO 3 (0.4);ZnSO 4 ·7H 2 O(1.0);MnSO 4 ·5H 2 O(1.0);CuSO 4 ·5H 2 O(7.0);CoCl 2 ·6H 2 O(1.0); and NiSO 4 ·7H 2 O(1.0), the concentration is in mg / L.
[0047] (2) As the electron donor after separate preparation, NaNO 2 , Na 2 S 2 o 3 and Na 2 Concentrate of S with a concentration of 200 mg / mL;
[0048] (3) Select several kinds of electron donor concentrates obtained in step (2), according to 5.0g / L NaNO 2 or 1.7g / LNa 2 S 2 o 3 or 3.3g / L Na 2 The amount of any electron donor in S is added to the culture medium obtained in step (1) by adding the electro...
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