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Application of quantitative detection of cpt1a gene or protein in prognosis of esophageal squamous cell carcinoma

A technique for quantitative detection and esophageal squamous cell carcinoma, which is applied in the direction of microbial determination/inspection, measuring devices, instruments, etc., and can solve the problems of failure to apply clinical detection

Active Publication Date: 2015-08-05
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Although the above-mentioned DNA molecular markers related to the prognosis of esophageal squamous cell carcinoma have been discovered, they have not been applied to clinical detection

Method used

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  • Application of quantitative detection of cpt1a gene or protein in prognosis of esophageal squamous cell carcinoma
  • Application of quantitative detection of cpt1a gene or protein in prognosis of esophageal squamous cell carcinoma
  • Application of quantitative detection of cpt1a gene or protein in prognosis of esophageal squamous cell carcinoma

Examples

Experimental program
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Effect test

Embodiment 1

[0074] Retrospective study: DNA was extracted from 151 esophageal squamous cell carcinoma specimens (from Cancer Hospital, Chinese Academy of Medical Sciences), and the copy number of CPT1A gene was detected by Real-time PCR method and analyzed.

[0075] DNA copy number analysis: DNeasy Blood & Tissue Kit (Qiagen) kit was used to extract tumor tissue DNA according to the instructions, and the tumor cell content in the tumor tissue was required to be greater than 50%. Using Power SYBR The Green PCR Master Mix (Applied Biosystems) kit was used for real-time quantitative PCR (Real-time PCR) detection. The reaction system is 20μl: 10μl 2×Power SYBR Green PCR Master Mix, 2 μl of tumor tissue DNA (5ng / μl), 1 μl of primers (upper and downstream primers each 10 μM), 7 μl of nuclease-free water.

[0076] The reaction program is: 95°C for 10 minutes; 95°C for 15 seconds, 60°C for 1 minute, 40 cycles.

[0077] Real-time PCR results using 2 -ΔΔCt The internal reference gene was GAPD...

Embodiment 2

[0086] Retrospective study: DNA was extracted from 84 esophageal squamous cell carcinoma specimens (from Lin County, Henan Province), and the copy number of CPT1A gene was detected and analyzed by Real-time PCR method. The DNA copy number analysis method is the same as in Example 1. The result is as figure 2 and shown in Table 2.

[0087] Table 2. Multivariate regression analysis

[0088]

[0089] It can be seen from the results that patients with increased copy number (gain) of the CPT1A gene have a shorter postoperative survival time than patients without gain.

Embodiment 3

[0091] Retrospective study: 73 cases of esophageal squamous cell carcinoma tumor specimens were prepared for tissue microarray, and the protein expression level of CPT1A gene was detected and analyzed by immunohistochemical method.

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Abstract

The invention belongs to the field of clinical prognosis in molecular biology and relates to an application of quantitative detection of a CPT1A gene or protein to prognosis of esophageal squamous cell carcinomas, and in particular relates to an application of a quantitative detection reagent of a CPT1A gene or protein in preparation of a prognosis kit of esophageal squamous cell carcinomas, a kit for prognosis of esophageal squamous cell carcinomas and a method for detecting CPT1A gene copy numbers or protein expression levels of esophageal squamous cell carcinomas patients. CPT1A gene copy number increase or protein overexpression prompts that the postoperative life cycles of the esophageal squamous cell carcinomas patients are short.

Description

technical field [0001] The invention belongs to the fields of molecular biology and clinical prognosis judgment, and specifically relates to the application of the copy number or protein expression level of CPT1A gene in the prognosis judgment of esophageal squamous cell carcinoma. The invention also relates to a detection method for the CPT1A gene copy number and protein expression level. Background technique [0002] Esophageal squamous cell carcinoma (esophageal squamous cell carcinoma) is one of the most common malignant tumors of the digestive tract in humans. Esophageal tissue cells are stimulated by some biological, physical, chemical and other factors to abnormally proliferate until the pathological characteristics of cancerous esophageal squamous cell carcinoma. Progressive dysphagia is its main clinical manifestation. The pathogenesis of esophageal squamous cell carcinoma is still unclear. [0003] There are more than 450,000 new cases and more than 380,000 death...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/48G01N33/573
Inventor 王明荣史志周徐昕郝佳洁
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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