A marker for judging the prognosis of colorectal cancer and its application

A colorectal cancer and prognosis technology, applied in the field of medical biotechnology and medical testing, can solve the problems of lost treatment opportunities, poor prognosis, hidden onset of colon cancer, etc.

Active Publication Date: 2021-10-26
蔡剑平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The onset of colon cancer is insidious, there are often no obvious clinical symptoms in the early stage, and the disease develops slowly. Most colorectal cancer patients are already in the advanced stage of tumor when they are diagnosed, and the best time for treatment has been lost, resulting in a five-year survival rate of less than 20%. %, poor prognosis

Method used

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  • A marker for judging the prognosis of colorectal cancer and its application
  • A marker for judging the prognosis of colorectal cancer and its application
  • A marker for judging the prognosis of colorectal cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] I. Materials and Methods

[0035] Experimental material

[0036] (1) 44 Normal tissue from the corresponding cancer tissue and corresponding carcinoma (provided by the First Affiliated Hospital of Jiamusi)

[0037] (2) Colon cancer tissue chip - 87 cases of cancer tissue and corresponding cancer normally tissue (Shanghai Core Super Syndrome Technology Co., Ltd.) patients did not pass through radiotherapy and chemotherapy before colorectal cancer

[0038] (3) Anti-MTH1 antibody (ABCAM), anti-MTH3 antibody (ABCAM), anti-Nudt5 antibody (abcam), anti-MTH2 antibody (abclonal), anti-GAPDH antibody (ABCAM)

[0039] 2. Experimental method

[0040] (1) The expression of MTH1, MTH2, MTH3, NUDT5 in normal tissue of colorectal cancer tissue and corresponding cancer tissue is analyzed by Western Blotting, and analyzed the amount of protein expression and clinical pathological information (such as gender, age, position). Correlation between tumor size, clinical staging, degree of differe...

Embodiment 2

[0092] Example 2. Kit of progress and prognosis of colorectal cancer

[0093] I. Composition of the kit

[0094] Anti-MTH1 antibody (ABCAM)

[0095] 2. Anti-MTH2 antibody (Abclonal)

[0096] 3. Anti-MTH3 antibody (ABCAM)

[0097] 4. Anti-Nudt5 antibody (ABCAM)

[0098] 2. How to use

[0099] Referring to Example 1 "The amount of expression of MTH1, MTH2, MTH3, NUDT5 in 87 cases of cancer tissue (tissue chip) by immunohistochemistry (tissue chip)" or "detection 44 through Western blotting 44 pair colorectal cancer The expression of MTH1, MTH2, MTH3, NUDT5 in the normal tissue of cancer is "."

Embodiment 3

[0100] Example 3. Kit for detecting a prognosis of colorectal cancer

[0101] I. Composition of the kit

[0102] Anti-MTH1 antibody (ABCAM)

[0103] 2. Anti-MTH2 antibody (Abclonal)

[0104] 3. Anti-Nudt5 antibody (ABCAM)

[0105] 2. How to use

[0106] Referring to Example 1 "The amount of expression of MTH1, MTH2, MTH3, NUDT5 in 87 cases of cancer tissue (tissue chip) by immunohistochemistry (tissue chip)" or "detection 44 through Western blotting 44 pair colorectal cancer The expression of MTH1, MTH2, MTH3, NUDT5 in the normal tissue of cancer is "."

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Abstract

The invention relates to a marker for judging the prognosis of colorectal cancer and its application, belonging to the technical fields of medical biotechnology and medical testing. Use of MutT-associated proteins as prognostic biomarkers for colorectal cancer patients. The MutT-related protein of the present invention can effectively distinguish the postoperative survival period of colorectal cancer patients, thereby providing a new approach for the prognosis diagnosis of colorectal cancer, and providing a reference for clinicians to analyze the condition of colorectal cancer.

Description

Technical field [0001] The present invention relates to markers for a determination of the prognosis of colorectal cancer and its application, of biotechnology and medical technology field of medical testing. Background technique [0002] Of colorectal cancer (colorectal cancer, CRC) is a malignant cancer due to the colonic mucosa epithelium in the role of environmental and genetic factors that cause cancer and other, is one of the most common gastrointestinal malignancy in the world, living in the incidence of malignant third cancer mortality in fourth place, about 1.2 million new cases each year, a serious threat to human health. A higher incidence of colorectal cancer in Asia, especially China, and the main risk factors as age, family history, diet and unhealthy living habits and so on. Insidious onset colon cancer early often without obvious clinical symptoms and slow progression of the disease, the majority of colorectal cancer have tumors in patients with advanced stage whe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57446
Inventor 蔡剑平李瑾
Owner 蔡剑平
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