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Marker for determining prognosis of colorectal cancer and application thereof

A colorectal cancer and biomarker technology, applied in the field of medical biotechnology and medical testing, can solve the problems of colon cancer with hidden onset, less than 20% survival rate and poor prognosis

Active Publication Date: 2019-04-30
蔡剑平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The onset of colon cancer is insidious, there are often no obvious clinical symptoms in the early stage, and the disease develops slowly. Most colorectal cancer patients are already in the advanced stage of tumor when they are diagnosed, and the best time for treatment has been lost, resulting in a five-year survival rate of less than 20%. %, poor prognosis

Method used

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  • Marker for determining prognosis of colorectal cancer and application thereof
  • Marker for determining prognosis of colorectal cancer and application thereof
  • Marker for determining prognosis of colorectal cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Materials and methods

[0035] 1. Experimental materials

[0036] (1) 44 pairs of colorectal cancer tissues and corresponding adjacent normal tissues (provided by the First Affiliated Hospital of Jiamusi University)

[0037] (2) Colon cancer tissue chip - 87 cases of cancer tissue and corresponding adjacent normal tissue (Shanghai Xinchao Biotechnology Co., Ltd.) patients who had not undergone radiotherapy and chemotherapy before radical operation for colorectal cancer

[0038] (3) Anti-MTH1 antibody (Abcam), anti-MTH3 antibody (Abcam), anti-NUDT5 antibody (Abcam), anti-MTH2 antibody (Abclonal), anti-GAPDH antibody (Abcam)

[0039] 2. Experimental method

[0040] (1) The expression levels of MTH1, MTH2, MTH3, and NUDT5 in 44 pairs of colorectal cancer tissues and corresponding adjacent normal tissues were detected by Western Blotting, and the expression levels of these proteins were analyzed with clinicopathological data (such as gender, age, location) , tumor siz...

Embodiment 2

[0092] Example 2. A kit for detecting the progression and prognosis of colorectal cancer

[0093] 1. Kit composition

[0094] 1. Anti-MTH1 antibody (Abcam)

[0095] 2. Anti-MTH2 antibody (Abclonal)

[0096] 3. Anti-MTH3 antibody (Abcam)

[0097] 4. Anti-NUDT5 antibody (Abcam)

[0098] 2. How to use

[0099] See Example 1 "Detecting the expression levels of MTH1, MTH2, MTH3, and NUDT5 in 87 cases of cancer tissues and corresponding paracancerous normal tissues (tissue chips) by immunohistochemistry" or "Detecting the expression levels of 44 pairs of colorectal cancer tissues and The expression levels of MTH1, MTH2, MTH3 and NUDT5 in the corresponding adjacent normal tissues".

Embodiment 3

[0100] Example 3. A kit for detecting the prognosis of colorectal cancer

[0101] 1. Kit composition

[0102] 1. Anti-MTH1 antibody (Abcam)

[0103] 2. Anti-MTH2 antibody (Abclonal)

[0104] 3. Anti-NUDT5 antibody (Abcam)

[0105] 2. How to use

[0106] See Example 1 "Detecting the expression levels of MTH1, MTH2, MTH3, and NUDT5 in 87 cases of cancer tissues and corresponding paracancerous normal tissues (tissue chips) by immunohistochemistry" or "Detecting the expression levels of 44 pairs of colorectal cancer tissues and The expression levels of MTH1, MTH2, MTH3 and NUDT5 in the corresponding adjacent normal tissues".

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Abstract

The invention, which belongs to the fields of medical biotechnology and medical examination technology, relates to a marker for determining the prognosis of the colorectal cancer and an application thereof. The MutT-related protein is used as a biomarker for prognosis of a patient with the colorectal cancer. With the MutT-related protein, the postoperative survival period of the patient with the colorectal cancer can be distinguished effectively, so that the novel path is provided for the prognosis diagnosis of the colorectal cancer and the reference basis is provided for the colorectal canceranalysis by the clinician.

Description

technical field [0001] The invention relates to a marker for judging the prognosis of colorectal cancer and its application, belonging to the technical fields of medical biotechnology and medical testing. Background technique [0002] Colorectal cancer (CRC) is the malignant transformation of colonic mucosal epithelium under the action of various carcinogenic factors such as environment and genetics. It is one of the most common malignant tumors of the digestive tract. Tumor ranks third, and the death rate ranks fourth. There are about 1.2 million new cases every year, which seriously threaten human health. The incidence of colorectal cancer is relatively high in Asia, especially in China, which is mainly related to risk factors such as age, family history, unhealthy diet and living habits. The onset of colon cancer is insidious, there are often no obvious clinical symptoms in the early stage, and the disease develops slowly. Most colorectal cancer patients are already in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57446
Inventor 蔡剑平李瑾
Owner 蔡剑平
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