Marker for determining prognosis of colorectal cancer and application thereof
A colorectal cancer and biomarker technology, applied in the field of medical biotechnology and medical testing, can solve the problems of colon cancer with hidden onset, less than 20% survival rate and poor prognosis
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Embodiment 1
[0034] 1. Materials and methods
[0035] 1. Experimental materials
[0036] (1) 44 pairs of colorectal cancer tissues and corresponding adjacent normal tissues (provided by the First Affiliated Hospital of Jiamusi University)
[0037] (2) Colon cancer tissue chip - 87 cases of cancer tissue and corresponding adjacent normal tissue (Shanghai Xinchao Biotechnology Co., Ltd.) patients who had not undergone radiotherapy and chemotherapy before radical operation for colorectal cancer
[0038] (3) Anti-MTH1 antibody (Abcam), anti-MTH3 antibody (Abcam), anti-NUDT5 antibody (Abcam), anti-MTH2 antibody (Abclonal), anti-GAPDH antibody (Abcam)
[0039] 2. Experimental method
[0040] (1) The expression levels of MTH1, MTH2, MTH3, and NUDT5 in 44 pairs of colorectal cancer tissues and corresponding adjacent normal tissues were detected by Western Blotting, and the expression levels of these proteins were analyzed with clinicopathological data (such as gender, age, location) , tumor siz...
Embodiment 2
[0092] Example 2. A kit for detecting the progression and prognosis of colorectal cancer
[0093] 1. Kit composition
[0094] 1. Anti-MTH1 antibody (Abcam)
[0095] 2. Anti-MTH2 antibody (Abclonal)
[0096] 3. Anti-MTH3 antibody (Abcam)
[0097] 4. Anti-NUDT5 antibody (Abcam)
[0098] 2. How to use
[0099] See Example 1 "Detecting the expression levels of MTH1, MTH2, MTH3, and NUDT5 in 87 cases of cancer tissues and corresponding paracancerous normal tissues (tissue chips) by immunohistochemistry" or "Detecting the expression levels of 44 pairs of colorectal cancer tissues and The expression levels of MTH1, MTH2, MTH3 and NUDT5 in the corresponding adjacent normal tissues".
Embodiment 3
[0100] Example 3. A kit for detecting the prognosis of colorectal cancer
[0101] 1. Kit composition
[0102] 1. Anti-MTH1 antibody (Abcam)
[0103] 2. Anti-MTH2 antibody (Abclonal)
[0104] 3. Anti-NUDT5 antibody (Abcam)
[0105] 2. How to use
[0106] See Example 1 "Detecting the expression levels of MTH1, MTH2, MTH3, and NUDT5 in 87 cases of cancer tissues and corresponding paracancerous normal tissues (tissue chips) by immunohistochemistry" or "Detecting the expression levels of 44 pairs of colorectal cancer tissues and The expression levels of MTH1, MTH2, MTH3 and NUDT5 in the corresponding adjacent normal tissues".
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