30 results about "Protein overexpression" patented technology

The present disclosure relates to methods for the early detection of ERG over-expression, gene rearrangements, and diagnosis and/or prognosis of prostate cancer using a combined ERG IHC-FISH assay.

The invention provides an XNDL2 gene, a protein, an overexpression vector, an acquisition method for rice resistant to rice sheath blight disease and application of the XNDL2 gene, belonging to the technical field of biology. The nucleotide sequence of the XNDL2 gene is shown as SEQ ID No. 1. The XNDL2 gene provided by the invention has the effect of resisting rice sheath blight disease. When theXNDL2 gene is introduced into rice, the rice capable of resisting rice sheath blight disease can be obtained.

A method for treating cancer comprising identifying a mammal that overexpresses breast cancer resistance protein; and administering to said mammal a pharmaceutical composition comprising a therapeutically effective amount of ixabepilone. In one aspect, the mammal is not administered an agent that is susceptible to breast cancer resistance protein overexpression resistance. In another aspect, the cancer is a solid tumor.

The invention relates to a medical application of recombinant protein Semaphorin3G in prevention and treatment of retinal diseases. According to the invention, the level of Sema3G protein in the retina is improved through adeno-associated virus mediated Sema3G protein overexpression in retinal vascular endothelial cells or injection of Sema3G recombinant protein into vitreous cavities, the pathological process of the OIR mouse retinopathy can be effectively improved, and the Sema3G protein can reduce the area of a vascular occlusion area in the retinopathy, and can also reduce the area of pathological neovascularization clusters, and relieve pathological phenotypes.

The invention discloses preparation and applications for mycobacterium tuberculosis PUP protein overexpression strains. BCG strains are cultivated, and the genome DNAs thereof are extracted. The genome DNAs of the BCG strains are taken as a template, and the PCR amplification of a mycobacterium tuberculosis Pup protein gene can be achieved; and sequencing and identification is performed. Escherichia coli-mycobacterium tuberculosis shuttle plasmid pMV361 is taken as a carrier, and recombinant shuttle plasmid pMV361-Pup : BCG is constructed and identified. An electroporation technology is utilized to transfer the constructed escherichia coli-mycobacterium tuberculosis shuttle plasmid pMV361 carrier (recombinant shuttle plasmid pMV361-Pup : BCG) into the BCG strains to construct and identifymycobacterium tuberculosis Pup protein overexpression strains which can be named as BCG : Pup strains. The strains can be used for studying the effects of ubiquitin-like proteasome system related protein PUP of mycobacterium tuberculosis on growth, drug resistance, permeability regulation, acid resistance and pathogenicity in hosts, and can also be used for the development and research of novel vaccines for intervening in and treating tuberculosis.

The invention discloses a method for inhibiting geminivirus infection by overexpression of arabidopsis ABI5 protein, which inhibits transcription of geminivirus coding genes by overexpression of plant transcription factor ABI5 protein and combination of ABI5 protein and a promoter of geminivirus through specificity, and realizes improvement of virus resistance of plants.

The invention provides a recombinant vector for promoting BNP (Brain Natriuretic Peptide) protein overexpression. The recombinant vector for promoting the BNP protein overexpression belongs to the field of biotechnology, and comprises a BNP coding sequence and a pAAV-TRE3G-P2A-GFP vector. The pAAV-TRE3G-P2A-GFP vector comprises AsiSI and MluI restriction enzyme cutting sites; and the BNP sequence is forward inserted into the restriction enzyme cutting sites. The recombinant vector for promoting the BNP protein overexpression provided by the invention has the advantages of high transfection efficiency, capability of promoting the BNP protein overexpression and continuously stable expression, and is simple in construction method.

Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in E. coli. Unlike the conventional codon optimization or codon deoptimization method for solving the problem of rare codons, the present invention increases an expression efficiency of a target protein by merely having the ramp tag be fused with a target gene or independently expressed, without changing the original codon sequence, thereby allowing tRNA to be reused. Thus, the present invention provides a novel method for increasing recombinant protein expression which is capable of reducing costs and time in comparison to the codon optimization method that artificially synthesizes DNA sequences. Therefore, it is expected that the method of the present invention will be able to be used in production of high value-added pharmaceuticals or industrial enzymes.

A novel polypeptide which is useful in screening of an agent for improving insulin resistance and an agent for improving glucose metabolism, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, and a cell transformed with the expression vector are disclosed. The polypeptide is a protein expressed in skeletal muscle. When the protein is overexpressed, incorporation of sugar into a cell is inhibited. A method for screening of an agent for improving insulin resistance and/or an agent for improving glucose metabolism in which the polypeptide is used, and a method for producing a pharmaceutical composition for insulin resistance and/or glucose metabolism improvement comprising a substance obtained according to the method for screening as an active ingredient are also disclosed.

The invention discloses a method for improving the organic selenium synthesis ability of microorganisms based on sulfur-containing protein overexpression , and belongs to the technical field of biosynthesis of organic selenium. An exogenous sulfur-containing protein gene is introduced into a microbial genome, and the obtained genome is cultured in a high-selenium and low-sulfur environment. Sulfurin sulfur-containing amino acids (cysteine and methionine) is replaced by selenium during the overexpression of an exogenous sulfur-containing protein to synthesize selenosulfate amino acid in order to synthesize a selenoprotein, so efficient organic selenium synthesis of the microorganisms is achieved. The method is based on a substitution competition relationship between selenium and sulfur,a process for synthesizing the organic selenium by using the selenoprotein is provided, the sulfur-containing foreign protein is constructed in a biotechnical way, and the selenoprotein that is the organic selenium is formed in transferred microorganisms. The method can efficiently enrich selenium and ensure the safety.

The invention discloses a method for preparing glucaric acid through whole-cell transformation, and belongs to the technical field of biology. Escherichia coli is used as a production strain to heterologously express glucuronic acid dehydrogenase from different sources, escherichia coli cells with protein overexpression are obtained through high-density fermentation culture, and glucuronic acid isused as a substrate to produce glucaric acid. A conversion liquid has the advantages of single component, short production period, simple operation, low production cost, no potential safety hazard and the like.

The present invention relates to expression vectors containing in vivo urea cycle enzyme gene; transformants thereof; and the use of transformants for protein overexpression. During the overexpression of protein, if the animal cell transformed with expression vectors of the present invention is used, amounts of ammonia accumulation within cell culture medium will decrease and cell growth rate will increase, thus, it is advantageous to be capable of obtaining desired protein in high yields.