30 results about "Protein overexpression" patented technology

The present invention relates to small molecule antagonists of Bcl-2 family proteins such as Bcl-2 and / or Bcl-XL. In particular, the present invention provides non-peptide cell permeable small molecules (e.g., tricyclo-dibenzo-diazocine-dioxides) that bind to a pocket in Bcl-2 / Bcl-XL that block the anti-apoptotic function of these proteins in cancer cells and tumor tissues exhibiting Bcl-2 protein overexpression. In preferred embodiments, the small molecules of the present invention are active at the BH3 binding pocket of Bcl-2 family proteins (e.g., Bcl-2, Bcl-XL, and Mcl-1). The compositions and methods of the present invention are useful therapeutics for cancerous diseases either alone or in combination with chemotherapeutic or other drugs.

A novel polypeptide which is useful in screening of an agent for improving insulin resistance and an agent for improving glucose metabolism, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, and a cell transformed with the expression vector are disclosed. The polypeptide is a protein expressed in skeletal muscle. When the protein is overexpressed, incorporation of sugar into a cell is inhibited.A method for screening of an agent for improving insulin resistance and / or an agent for improving glucose metabolism in which the polypeptide is used, and a method for producing a pharmaceutical composition for insulin resistance and / or glucose metabolism improvement comprising a substance obtained according to the method for screening as an active ingredient are also disclosed.

The invention discloses a biosynthesis method of α-lipoic acid, an engineering strain and a preparation method thereof. The biosynthesis method is as follows: cloning Escherichia coli lipD gene, lplA gene, metK gene and lipA lipoic acid synthase gene; constructing lipoic acid synthase gene Overexpression vector of octanoic acid domain protein; tandem cloning of lplA into a vector for high-efficiency expression domain protein to ensure complete octanoylation of domain protein; construction of lipA or tandem expression vector with metK; transform the above four vectors into suitable In Escherichia coli strains, media composition and culture conditions were adjusted and compared to promote the conversion of octanoylation domain proteins to lipoylation. The α-lipoic acid preparation method of the present invention has the advantages of combining low-value chemical raw materials with pure biological production methods, less toxic substances in the production process, and less environmental pollution. Its synthetic lipoic acid has high safety and high yield; the obtained α-lipoic acid activity is high.

Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in E. coli. Unlike the conventional codon optimization or codon deoptimization method for solving the problem of rare codons, the present invention increases an expression efficiency of a target protein by merely having the ramp tag be fused with a target gene or independently expressed, without changing the original codon sequence, thereby allowing tRNA to be reused. Thus, the present invention provides a novel method for increasing recombinant protein expression which is capable of reducing costs and time in comparison to the codon optimization method that artificially synthesizes DNA sequences. Therefore, it is expected that the method of the present invention will be able to be used in production of high value-added pharmaceuticals or industrial enzymes.

The invention belongs to the field of clinical prognosis in molecular biology and relates to an application of quantitative detection of a CPT1A gene or protein to prognosis of esophageal squamous cell carcinomas, and in particular relates to an application of a quantitative detection reagent of a CPT1A gene or protein in preparation of a prognosis kit of esophageal squamous cell carcinomas, a kit for prognosis of esophageal squamous cell carcinomas and a method for detecting CPT1A gene copy numbers or protein expression levels of esophageal squamous cell carcinomas patients. CPT1A gene copy number increase or protein overexpression prompts that the postoperative life cycles of the esophageal squamous cell carcinomas patients are short.

The present invention relates to expression vectors containing in vivo urea cycle enzyme gene; transformants thereof; and the use of transformants for protein overexpression. During the overexpression of protein, if the animal cell transformed with expression vectors of the present invention is used, amounts of ammonia accumulation within cell culture medium will decrease and cell growth rate will increase, thus, it is advantageous to be capable of obtaining desired protein in high yields.

The invention provides a kind of Y related to stress resistance of lentil beans 2 K 4 dehydrin MrY 2 K 4 And its coding gene and its application. The present invention clones and isolates a new YnKn-type dehydrin protein and its coding gene MrY from lentil beans by RT-PCR technology 2 K 4 , and use prokaryotic expression to express MrY 2 K 4 After the gene coding region is connected to the E. coli expression vector, the prokaryotic expression vector is transferred into the E. coli expression strain, and MrY is realized after induction with IPTG 2 K 4 Protein overexpression in E. coli, the results showed that MrY 2 K 4 The overexpression of the protein has a protective effect on Escherichia coli under adversity stress, therefore, the gene can be used to improve the salt resistance and heat resistance of plants and microorganisms through biotechnology methods.