Phlomis umbrosa root total-drop triterpene composition and extraction method and application thereof

An extraction method and nortriterpene technology, which can be applied in the directions of drug combinations, pharmaceutical formulations, and medical preparations containing active ingredients, etc., can solve the problems of troublesome preparation process, difficult development and utilization, and small sample volume.

Inactive Publication Date: 2012-09-26
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method generally adopts organic solvent extraction, silica gel column chromatographic separation, high-performance liquid chromatographic separation, etc. to separate triterpenoid components from Silenseed hemp. Research

Method used

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  • Phlomis umbrosa root total-drop triterpene composition and extraction method and application thereof
  • Phlomis umbrosa root total-drop triterpene composition and extraction method and application thereof
  • Phlomis umbrosa root total-drop triterpene composition and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The total nortriterpenoid composition of Rhizoma Rhizoma in this example contains the following weight percentages of compounds 1-6: compound 1: 17.51%, compound 2: 12.35%, compound 3: 10.24%, compound 4: 9.31%, compound 5: 4.57%, compound 6: 4.08%, and the rest are lower content of nortriterpenes and impurities.

[0043] The total nortriterpenes composition of Rhizoma Phragmatis in this embodiment is extracted by the following method:

[0044] 1) Take 1 kg of dry and crushed 60-mesh four-lens hemp (rough persimmon root), add 20 kg of 95% ethanol to extract under reflux conditions for 5 hours, add 95% ethanol to the filtered residue and extract under reflux conditions for 4 hours, Repeat the extraction for a total of three times under reflux, combine the extracts (filtered filtrate), and reclaim ethanol under reduced pressure to obtain 180 g of extract;

[0045] 2) Fully dissolve the extract with 720 grams of petroleum ether-ethyl acetate with a volume ratio of 2:1, fi...

Embodiment 2

[0052] The total nortriterpenoid composition of Rhizoma Rhizoma in this embodiment contains the following weight percentages of compounds 1-6: compound 1: 14.36%, compound 2: 13.65%, compound 3: 11.24%, compound 4: 8.12%, compound 5: 4.21%, compound 6: 3.59%, and the rest are lower content of nortriterpenes and impurities.

[0053] The total nortriterpenes composition of Rhizoma Phragmatis in this embodiment is extracted by the following method:

[0054] 1) Take 2 kilograms of dry and crushed 60-mesh four-lens hemp (rough persimmon root), add 30 kilograms of 90% ethanol to extract under reflux conditions for 4 hours, add 90% ethanol to the filtered residue and extract under reflux conditions for 3 hours, Repeat the extraction for a total of reflux extraction four times, combine the extracts (filtered filtrate), recover ethanol under reduced pressure to obtain 395 g of extract;

[0055] 2) The extract was fully dissolved with 1975 g of petroleum ether-ethyl acetate with a volu...

Embodiment 3

[0062] The total nortriterpenoid composition of Rhizoma Rhizoma in this embodiment contains the following weight percentages of compounds 1-6: compound 1: 13.23%, compound 2: 14.05%, compound 3: 12.04%, compound 4: 8.24%, compound 5: 4.87%, compound 6: 4.25%, and the rest are lower content of nortriterpenes and impurities.

[0063] The total nortriterpenes composition of Rhizoma Phragmatis in this embodiment is extracted by the following method:

[0064] 1) Take 3 kilograms of dry and crushed 60-mesh four-lens hemp (rough persimmon root), add 30 kilograms of 85% ethanol to extract under reflux conditions for 3 hours, and then extract the filtered residue with 85% ethanol for 5 hours under reflux conditions, Repeat the extraction for a total of five times of reflux extraction, combine the extracts (filtered filtrate), and reclaim ethanol under reduced pressure to obtain 624 g of extract;

[0065] 2) Fully dissolve the extract with 1872 g of petroleum ether-ethyl acetate with a...

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Abstract

The invention discloses a phlomis umbrosa root total-drop triterpene composition which comprises six concrete-drop triterpene compounds derived from the general formula A, and the formula shows the six major ingredients of the phlomis umbrosa root total-drop triterpene composition extracted in the invention. The in-vitro anti-tumor cell test indicates that: the phlomis umbrosa root total-drop triterpene composition disclosed by the invention has a relatively strong effect on suppressing tumor cells, and can be further developed and utilized as a candidate anti-tumor medicine, thereby laying a foundation for the further development and research.

Description

technical field [0001] The invention relates to a composition of total nortriterpenoids of Cortella Phyllostachys, and also relates to an extraction method for extracting total nortriterpenes, and an application in preparation of antitumor drugs. Background technique [0002] Malignant tumor is one of the main diseases that endanger human health today. Together with cardiovascular disease and cerebrovascular disease, it constitutes the "three major killers" that pose a great threat to human life and health. In recent years, tumor chemotherapy has made great progress, but the therapeutic effect on solid tumors is still unsatisfactory; and many anti-tumor drugs will inevitably damage normal tissues and organs while killing tumor cells. Natural medicines and traditional Chinese medicines are important sources of modern innovative drug discovery, with outstanding advantages such as diverse chemical structures, multi-linked mechanisms of action, and low toxicity and side effects....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56A61P35/00
Inventor 刘普邓瑞雪梁菊段文录尹卫平柴元武
Owner HENAN UNIV OF SCI & TECH
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