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Spinner bottle production method of inactivated singapore grouper iridovirus (SGIV) vaccine

A technology of iridescent viruses and inactivated vaccines, applied in biochemical equipment and methods, antiviral agents, viruses/bacteriophages, etc., can solve the problems of large-scale applications, high production costs, and high labor intensity, and achieve low production costs , Stable batch-to-batch quality and low labor intensity

Active Publication Date: 2013-12-25
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method only prepares cell-inactivated vaccines in the laboratory, and its production cost is high, labor-intensive, and cannot be applied on a large scale in industry.

Method used

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  • Spinner bottle production method of inactivated singapore grouper iridovirus (SGIV) vaccine
  • Spinner bottle production method of inactivated singapore grouper iridovirus (SGIV) vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Cell growth medium: Leibovitz's-15 (L15) medium, prepared according to the company's product instructions, and add 0.266% NaCl, 5mM HEPES to adjust the pH to 7.2~7.6. In this example, adjust the pH to 7.6, and filter through a 0.22 μm filter membrane , add fetal bovine serum before use, so that its volume fraction is 8-10%, and the present embodiment contains 10% fetal bovine serum by volume fraction.

[0021] 1. Vial culture of grouper spleen tissue cells. Take the grouper spleen tissue cells covered with a single layer, remove the original culture medium, rinse once with phosphate buffered saline (PBS), add 0.25% trypsin to digest for 1-2 minutes, then add the above cell growth solution, After blowing evenly, divide into 3 cell culture flasks for culture and expansion at 28°C.

[0022] 2. Spinner bottle culture of cells: take the above 15 75cm cells covered with monolayer grouper spleen tissue cells 2 For cell culture flasks, remove the original culture medium, rins...

Embodiment 2

[0026] Cell growth medium: Leibovitz's-15 (L15) medium, prepared according to the company's product instructions, and add 0.266% NaCl, 5mM HEPES to adjust the pH to 7.2~7.6. In this example, adjust the pH to 7.2 and filter through a 0.22 μm filter membrane , add fetal bovine serum before use, so that its volume fraction is 8-10%, and the volume fraction of this embodiment is 8% fetal bovine serum.

[0027] 1. Vial culture of grouper spleen tissue cells. Take the grouper spleen tissue cells covered with a single layer, remove the original culture medium, rinse once with phosphate buffered saline (PBS), add 0.25% trypsin to digest for 1-2 minutes, then add the above cell growth solution, After blowing evenly, divide into 3 cell culture flasks for culture and expansion at 28°C.

[0028] 2. Spinner bottle culture of cells: take the above 10 75cm cells covered with monolayer grouper spleen tissue cells 2For cell culture flasks, remove the original culture medium, rinse once with ...

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Abstract

The invention discloses a spinner bottle production method of inactivated singapore grouper iridovirus (SGIV) vaccine. The production method comprises spinner bottle culture of cells, amplification and harvest of viruses and inactivation of the viruses. By using the spinner bottle production method of the inactivated SGIV vaccine disclosed by the invention, the inactivated SGIV vaccine can be produced on a large scale, and the production cost is low and the labor intensity is small since the production is industrial streamline production; after vaccine inspection on the produced inactivated SGIV vaccine, the inactivated SGIV vaccine is safe from the aspect of cell level and fish level; vaccine efficacy detection proves that the relative protection rate of the inactivated SGIV vaccine provided by the invention is 87.0-95.7%, so that inactivated SGIV vaccine provides obvious protection for immune grouper, the immune protection efficacy is good and quality between batches is stable.

Description

technical field [0001] The invention belongs to the field of aquatic animal vaccines, and in particular relates to a bottle-rotating production method of grouper iridescent virus (SGIV) inactivated vaccine. Background technique [0002] Grouper meat is delicious and has high protein content. It is a kind of edible fish with high economic value, and it is also an excellent fish species that earns foreign exchange in my country. However, in recent years, with the expansion of breeding scale and the deteriorating breeding environment, various diseases, especially viral diseases, frequently broke out, causing significant economic losses. Iridescent virus is an important pathogen of grouper, which can cause fish mortality ranging from 30% (in adult fish) to 100% (juvenile stage). In Asia, especially in high water temperatures above 22°C, this virus is very likely to infect rare seawater fish cultured in China, Japan and Southeast Asia, such as grouper, barramundi, red sea bream,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61P31/20C12N7/00
Inventor 秦启伟欧阳征亮魏京广黄晓红
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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