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Construction and preparation of three-dimensional bionic electropolarized gradient pore nerve conduit

A nerve conduit and gradient hole technology, applied in the construction and preparation of nerve conduits, achieves the effects of simple preparation methods, directional adhesion, and good stability

Inactive Publication Date: 2014-07-02
武汉渝联生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, any single degradable material cannot meet the above requirements. Only by making full use of the biologically active elements of natural polymers and the good mechanical processing properties of synthetic polymers, facilitating mass production and low cost, can natural polymers and Synthetic polymer structure hybridization and complementary advantages can make the peripheral nerve regeneration catheter prepared with more performance

Method used

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  • Construction and preparation of three-dimensional bionic electropolarized gradient pore nerve conduit
  • Construction and preparation of three-dimensional bionic electropolarized gradient pore nerve conduit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation steps of the three-dimensional bionic electric polarization gradient hole nerve conduit are as follows:

[0032](1) Place β-tricalcium phosphate (β-TCP) powder (10-100nm) in an alumina ring with a diameter of 2 cm and a height of 0.5 cm, under the action of a 2kvcm-1 DC electric field, electrode in air at 100°C After 1 hour of chemical reaction, the electrodeposited β-TCP was obtained, which was taken out for later use;

[0033] (2) At room temperature, using ethyl acetate as a solvent, add polylactic acid (PDLLA) and electropolarized β-TCP respectively to make a mixed solution of polylactic acid with a mass concentration of 5% and electropolarized β-TCP with a mass concentration of 5%. ;

[0034] (3) Use magnetic stirring to completely disperse the above mixed solution, then slowly pour the mixed solution into a glass mold, at room temperature, use the solvent evaporation method for 12 hours, vacuum dry for 12 hours, and then peel off the core material...

Embodiment 2

[0038] The preparation steps of the three-dimensional bionic electric polarization gradient hole nerve conduit are as follows:

[0039] (1) Place β-tricalcium phosphate (β-TCP) powder (10-100nm) in an alumina ring with a diameter of 4 cm and a height of 1.5 cm. Under the action of a DC electric field of 5kvcm-1, the electrode is placed in the air at 200°C After 2 hours of chemical reaction, the polarized β-TCP was obtained, and it was taken out for later use;

[0040] (2) At room temperature, using chloroform as a solvent, add polylactic acid (PDLLA) and electropolarized β-TCP respectively to prepare a mixed solution of polylactic acid with a mass concentration of 10% and electropolarized β-TCP with a mass concentration of 6%;

[0041] (3) Use magnetic stirring to completely disperse the above mixed solution, then slowly pour the mixed solution into a glass mold, at room temperature, use the solvent evaporation method for 24 hours, vacuum dry for 24 hours, and then peel off th...

Embodiment 3

[0045] The preparation steps of the three-dimensional bionic electric polarization gradient hole nerve conduit are as follows:

[0046] (1) Place β-tricalcium phosphate (β-TCP) powder (10-100nm) in an alumina ring with a diameter of 6 cm and a height of 2 cm, under the action of a DC electric field of 8kvcm-1, electrode in air at 300°C After 1 hour of chemical reaction, the electrodeposited β-TCP was obtained, which was taken out for later use;

[0047] (2) At room temperature, using acetone as a solvent, add polylactic acid (PDLLA) and electropolarized β-TCP respectively to prepare a mixed solution of polylactic acid with a mass concentration of 15% and electropolarized β-TCP with a mass concentration of 8%;

[0048] (3) Use magnetic stirring to completely disperse the above mixed solution, then slowly pour the mixed solution into a glass mold, at room temperature, use the solvent evaporation method for 36 hours, vacuum dry for 36 hours, and then peel off the core material fr...

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Abstract

The invention provides construction and preparation of a three-dimensional bionic electropolarized gradient pore nerve conduit. The nerve conduit is an electropolarized beta-tricalcium phosphate / polylactic acid / hyaluronic acid / chitosan / nerve growth factor nerve conduit, and is prepared by the steps of: preparing a uniformly dispersed electropolarized beta-tricalcium phosphate and polylactic acid core material by using polylactic acid as a substrate material and adding the electropolarized beta-tricalcium phosphate; and preparing a three-dimensional bionic electropolarized gradient pore nerve conduit with component and pore structure double gradient by attaching the hyaluronic acid, the chitosan and the nerve growth factor to the surface of the core material by virtue of electrostatic self-assembly and coupling crosslinking, wherein the length is between 10 and 100mm, the inner diameter is between 1 and 6mm, the wall thickness is between 0.5 and 2mm, and the outer tube wall is of a three-dimensional network structure. The nerve conduit is applied to repairing of a peripheral nervous system, induction of peripheral nerve regeneration and in-vitro culture of nerve cells in tissue engineering. The three-dimensional bionic electropolarized gradient pore nerve conduit has the advantages of simple preparation method, low cost, environmental friendliness and high economical benefit, and is applicable to industrial production.

Description

technical field [0001] The invention belongs to the field of nerve conduit construction and preparation thereof, in particular to the construction and preparation of a three-dimensional bionic electric polarization gradient hole nerve conduit. Background technique [0002] Peripheral nerve defect is a common clinical disease, about 2.8% of trauma patients lead to peripheral nerve injury, and severe cases will be permanently disabled. Hundreds of thousands of cases in my country need nerve repair every year, but the repair effect is not satisfactory, so the regeneration and functional recovery of peripheral nerve injury has always been a hot topic in the field of neuroscience. At present, autologous nerve transplantation, as the gold standard for peripheral nerve injury, is the most classic method of bridging and repairing nerve defects, but it is not the most ideal method. In addition to the limited source of donors, it will cause partial function loss of the donor site ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/18A61L27/12A61L27/54
Inventor 徐海星刘慧许沛虎龙倩黄志军张喻王玲李映萱王玉陈慧
Owner 武汉渝联生物科技有限公司
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