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Method for rapidly identifying and screening anti-blight banana seedling

A banana fusarium wilt and banana technology, which is applied in the field of rapid identification and screening of banana fusarium wilt disease-resistant seedlings, can solve the problems of affecting the accuracy of test results, unfavorable screening of disease-resistant seedlings, and long identification period, so as to save space and test The effect of cost, easy manual control, and shortened appraisal period

Inactive Publication Date: 2013-04-17
东莞市香蕉蔬菜研究所
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Problems solved by technology

[0003] At present, the identification of banana fusarium wilt-resistant seedlings commonly used in my country generally adopts the method of pot plant inoculation with soil, fine sand or substrate as the carrier, such as the most widely used pot plant root irrigation method and root soaking method, etc., the above-mentioned The potted plant inoculation method mainly has the following problems: 1. Since the interaction of soil microorganisms will affect the accuracy of the test results, large-scale soil disinfection must be carried out in advance, which is inconvenient to operate and is not conducive to a large number of screening of disease-resistant seedlings; 2. The identification period It usually takes more than 30 days for one identification; 3. The contact between seedlings and soil may be disturbed by other soil-borne diseases, which will affect the accuracy of test results

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  • Method for rapidly identifying and screening anti-blight banana seedling

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Embodiment Construction

[0023] In order to further understand the features, technical means and specific objectives and functions achieved by the present invention, the present invention will be further described in detail below in conjunction with specific embodiments.

[0024] The present invention is a method for quickly identifying and screening banana fusarium wilt-resistant seedlings, through three different resistances to Brazil (susceptible variety), Nongke No. 1 (medium-resistant variety), and Kangku No. 5 (high-resistant variety) In the identification of disease resistance of banana varieties, and compared with field disease evaluation.

[0025] 1. Prepare the inoculum. Based on the previous pathogenicity detection test, the pathogenic strain Fusarium oxysporum Cuban specialization No. 4 physiological race (Foc4) with strong pathogenicity was used. Physiological race No. 4 strain was inoculated in sterile potato lactose spore-forming culture medium (200g potato, 20g lactose, 1000mL water, ...

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Abstract

The invention discloses a method for rapidly identifying and screening anti-blight banana seedling. The method comprises the steps of establishment of banana seedling hydroponic system, inoculum preparation and inoculation, daily management of banana seedling, disease situation investigation and grading. Banana seedling stage inoculation and hydroponic identification method can effectively control interference due to differences in soil structure, nutrition, microorganism, etc., during banana seedling stage inoculation identification process, rapidly and accurately identify anti-blight bananaseedling, continuously identify large amount of tissue-cultured banana seedlings in small area, perform multiple times of identification in one year, improve test result reliability, shorten identification period, and save land occupation and test cost.

Description

technical field [0001] The invention relates to the technical field of agricultural plant protection, in particular to a method for rapidly identifying and screening banana fusarium wilt disease-resistant seedlings. Background technique [0002] Banana Fusarium wilt is an important disease distributed in major banana producing areas in the world. As banana Fusarium wilt is a typical soil-borne disease, its control is very difficult, and the application of disease-resistant varieties to control banana Fusarium wilt is currently the most economical and effective method. [0003] At present, the identification of banana fusarium wilt-resistant seedlings commonly used in my country generally adopts the method of pot plant inoculation with soil, fine sand or substrate as the carrier, such as the most widely used pot plant root irrigation method and root soaking method, etc., the above-mentioned The potted plant inoculation method mainly has the following problems: 1. Since the in...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01G1/00A01G31/00A01G7/06
Inventor 吕顺刘文清曾莉莎李洪波王芳周建坤
Owner 东莞市香蕉蔬菜研究所
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