Method for rapidly identifying and screening anti-blight banana seedling
A banana fusarium wilt and banana technology, which is applied in the field of rapid identification and screening of banana fusarium wilt disease-resistant seedlings, can solve the problems of affecting the accuracy of test results, unfavorable screening of disease-resistant seedlings, and long identification period, so as to save space and test The effect of cost, easy manual control, and shortened appraisal period
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[0023] In order to further understand the features, technical means and specific objectives and functions achieved by the present invention, the present invention will be further described in detail below in conjunction with specific embodiments.
[0024] The present invention is a method for quickly identifying and screening banana fusarium wilt-resistant seedlings, through three different resistances to Brazil (susceptible variety), Nongke No. 1 (medium-resistant variety), and Kangku No. 5 (high-resistant variety) In the identification of disease resistance of banana varieties, and compared with field disease evaluation.
[0025] 1. Prepare the inoculum. Based on the previous pathogenicity detection test, the pathogenic strain Fusarium oxysporum Cuban specialization No. 4 physiological race (Foc4) with strong pathogenicity was used. Physiological race No. 4 strain was inoculated in sterile potato lactose spore-forming culture medium (200g potato, 20g lactose, 1000mL water, ...
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