Method of judging distribution of ventral horn motor neurons in cervical segments of spinal cord of rat

A technology of motor neurons and spinal nerves, applied in the medical field, can solve problems such as rough research

Inactive Publication Date: 2012-11-14
SUZHOU HEALTH COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for judging the distribution of motoneurons in the cervical anterior horn of rat spinal cord, which solves the rough problem of the distribution of motoneurons in the cervical anterior horn of rat spinal cord in the prior art

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Embodiment 1

[0012] The present invention judges the method for the distribution of motor neurons in the cervical anterior horn of rat spinal cord, and the specific steps are as follows:

[0013] Step 1) Prepare experimental animals

[0014] 12 adult SD rats, weighing 230-250 g, clean grade, supplied by the Experimental Animal Center of Soochow University.

[0015] Step 2) Perfusion, fixation and material collection Twelve adult SD rats (8 males and 4 females) with a weight of 230±20 g were taken, and the left ventricle was punctured, perfused and fixed after intraperitoneal injection of anesthesia. The spinal cord with the spinal nerve root was taken out, after 12 hours of post-fixation, the spinal cord was taken out and sectioned, and put in 20% sucrose overnight.

[0016] Step 3) Slicing, staining, and image analysis Use filter paper to absorb the sucrose on the spinal cord, embed it, slice it in the coronal position with a LEICA cryostat, and slice the thickness of 40 μm. Two slices ...

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Abstract

The invention discloses a method of judging distribution of ventral horn motor neurons in cervical segments of the spinal cord of a rat. The method comprises the steps of: taking a plurality of frozen sections of cervical segments and cross sections of the spinal cord of an SD (Sprague Dawley) rat; dyeing the frozen sections by a cresyl violet and Pal-Weigert dyeing method; observing distribution of ventral horn motor neurons in the cervical segments of the spinal cord of SD rat by a light microscope technology; and researching the distribution characteristic of the ventral horn motor neurons in the cervical segments of the spinal cord of SD rat. According to the method of judging distribution of ventral horn motor neurons in cervical segments of the spinal cord of the rat provided by the invention, segments where cerebral nuclei appear and disappear in the spinal cord of the SD rat are refined to 1/4 section, thus showing that the appearance of different cerebral nuclei in same segments is probable, and a comparatively specific reference is provided for researching positioning of association of cerebral nuclei in different nerves and gray substances of the spinal cord.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for judging the distribution of motoneurons in the cervical anterior horn of rat spinal cord. Background technique [0002] Various atlases about the rat central nervous system, such as George paxinos's "Rat Brain Stereotaxic Atlas", describe the spinal cord in a rough manner, and there is no detailed division of the anterior horn motor neurons. [0003] There is no better related method in the prior art for judging the distribution of motoneurons in the cervical anterior horn of rat spinal cord. Contents of the invention [0004] The purpose of the present invention is to provide a method for judging the distribution of motoneurons in the cervical anterior horn of rat spinal cord, which solves the problem that the research on the distribution of motoneurons in the cervical anterior horn of rat spinal cord is relatively rough in the prior art. [0005] The method for judging t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00A61B19/00
Inventor 李东印
Owner SUZHOU HEALTH COLLEGE
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