Method for detecting latent tuberculosis infection state, and kit thereof
A tuberculosis latent and kit technology, applied in biochemical equipment and methods, microbial determination/examination, fluorescence/phosphorescence, etc., can solve problems such as indistinguishable, inaccurate diagnosis, and insolvency
Active Publication Date: 2013-02-13
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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Problems solved by technology
[0005] Both PPD and IGRA methods are cell-based immunodiagnostic methods. Although this type of method has been widely used, there is an obvious defect that has not been resolved. What they diagnose is not tuberculosis but tuberculosis infection.
This means that they cannot differentiate between active TB patients and those with latent TB infection, both groups are positive using the above methods and cannot be distinguished
Although patients with active tuberculosis have clinical symptoms, when patients with atypical clinical symptoms and suspected tuberculosis need to be diagnosed or are used in areas with high latent infection rates, accurate diagnosis is often not possible, which affects the application value of this method
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[0045] The gene chip of the present invention will be described and described in detail below through specific examples to better understand the scope of the present invention, but the following examples do not limit the scope of the present invention.
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The invention provides a method for detecting and discriminating the latent tuberculosis infection state, and a kit thereof. The contents of characteristic genes can be quantitatively detected through a real-time quantitative fluorescence amplification method, and the latent tuberculosis state is determined through comparing the multiple of a difference between an expression amount with the use of the tuberculin characteristic genes and an expression amount without the use of the tuberculin characteristic genes.
Description
technical field [0001] The invention relates to a method and kit for detecting tuberculosis latent infection, and also relates to a detection reagent used in the method. Background technique [0002] Tuberculosis, also known as tuberculosis and white plague, is a chronic infectious disease caused by tubercle bacillus infection. It is an ancient disease with a history of thousands of years and was once the main killer of human beings. Although effective anti-tuberculosis drugs have been discovered since the 1950s, and the epidemic has been controlled to a certain extent, in recent years, due to the neglect of tuberculosis in many countries, financial investment has been reduced, coupled with population growth and floating population. The increase and other factors have slowed the decline of tuberculosis prevalence, and even some countries and regions have rebounded. Therefore, the World Health Organization (WHO) declared a "global tuberculosis emergency" in 1993, and determin...
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Login to View More IPC IPC(8): C12Q1/68C12Q1/04G01N21/64
Inventor张文宏陈嘉臻路蝉伊张颖吴晶邵凌云王森
OwnerAFFILIATED HUSN HOSPITAL OF FUDAN UNIV