58 results about "Tuberculin" patented technology

An immunoadjuvant which can efficiently exhibit potent adjuvant activity and avoid conditions undesirable for living bodies, and comprises precipitates formed by coacervation of (a) a soluble protein (provided that a soluble protein contained in tuberculin is excluded), and (b) a mucopolysaccharide, and further comprises (c) a soluble protein contained in tuberculin wherein said (c) is coprecipitated with the precipitates.

A Safe and effective treatment methods to cure and curtail tuberculosis affliction described using high dose Vitamin C, and other known anti-mycobacterium tuberculosis drugs especially rifampicin administered intravenously for 6 weeks instead of 6 to 24 months of conventional treatment. Insulin is administered to induce moderate hypoglycemia to augment and add effectiveness of anti-tuberculosis drugs and Vitamin C. Invention also delivers the drugs directly to a tuberculin lesion through a catheter. An embodiment of the invention uses a nebulizer and other methods of administration of Vitamin C with anti-tuberculosis drugs, interferon Y−, Coley's vaccine, dinitrophenol hyperthermia, ozone therapy, Hydrogen peroxide therapy and artemisinin, combined with oxygen supplementation including autohemotherapy with ozone, hyperbaric therapy, and hypertheramia to increase the respiration of the M. tuberculosis bacteria which has a killing effect.

The invention relates to bovine type tuberculin standard substance and a preparation method thereof. The bovine type tuberculin standard substance is prepared by the following steps of: preparing bovine type tuberculin through processes of culturing, inactivating, filtering, purifying, and the like by utilizing mycobacteriumbovis separated in China; measuring the contents of protein, nucleic acid and polysaccharide in the bovine type tuberculin; and preparing the bovine type tuberculin standard substance by combining biological test measurement.

The invention discloses a tuberculin micro-needle patch as well as a preparation method and application thereof. The tuberculin micro-needle patch is prepared by the following steps: adding a micro-needle raw material solution, which is prepared by mixing tuberculin, a degradable material and sterile water, into a micro-needle template, wherein the concentration of the tuberculin is 20IU / ml to 200IU / ml and the mass percentage of the degradable material is 10 percent to 20 percent; after carrying out centrifuging treatment, removing part of the raw material on a needle hole of a micro-needle; adding a degradable material solution with the concentration of 5 percent to 10 percent; carrying out the centrifuging treatment again; putting the mixture into a drying oven and drying; then de-molding. The tuberculin micro-needle patch disclosed by the invention can be adhered to an adhesive tape and is directly stuck to skin through the adhesive tape so as to replace traditional injector intradermal vaccination; the total content of the tuberculin in the micro-needle patch is 0.05IU. The inoculation operation is simple and convenient and professional persons are not needed; the dosage of medicines is few and a phenomenon that bad skin response is easily generated by an injection skin test is avoided.

The invention discloses a method for preparing a pure protein derivative of tubercle bacillus by using a mycobacterium tuberculosis attenuated strain H37Ra, and belongs to the technical field of biological protein preparation. The mycobacterium tuberculosis attenuated strain CMCC93020 (H37Ra) is used as a strain, and a finished product of the pure protein derivative of the tubercle bacillus is obtained through amplification, inactivation, salting-out precipitation, degerming filtration, inspection, dilution and subpackaging. The defects in the prior art that strains have great harm to operators and the environment and have high requirements for production conditions (P3 production workshops) are overcome, and finally the protein which can be used for clinical diagnosis of tuberculosis, screening of bacillus calmette-guerin vaccine inoculation objects, monitoring of organism immune responses after bacillus calmette-guerin vaccine inoculation and epidemiological monitoring is obtained.

The invention provides a method for detecting and discriminating the latent tuberculosis infection state, and a kit thereof. The contents of characteristic genes can be quantitatively detected through a real-time quantitative fluorescence amplification method, and the latent tuberculosis state is determined through comparing the multiple of a difference between an expression amount with the use of the tuberculin characteristic genes and an expression amount without the use of the tuberculin characteristic genes.

The invention discloses a sieved tuberculin functional gene Rv0621 based on escherichia coli, which purifies and recombines Rv0621 to modulate the molecule of surface activity proof agent.

The invention relates to the field of medical test, in particular to a tuberculous infection diagnostic kit, a screening system and application of the kit. The kit contains mitogen and an antigen irritant. The antigen irritant is selected from the following tuberculin pure protein or three or more kinds of derivatives thereof, such as ESAT6, CFP10, TB7.7, MPT64, CFP21, EsxV, Rv1985C and Rv0222. The novel skin experiment for screening latent tuberculosis infection and active tuberculosis, provided by the invention, has the advantages of high specificity (identification of BCG vaccination and tuberculosis infection), high sensitivity (limited multiple antigen protein costimulation), small influence from individual basic immune differences and the like.

The invention discloses a method for establishing indirect ELISA (enzyme-linked immunosorbent assay) for detecting mycobacterium bovis Lprl, which comprises the following steps: preparing mycobacterium bovis Lprl protein, taking the purified protein as an antigen, and determining conditions such as optimal antigen coating concentration, serum dilution, serum incubation time, secondary antibody dilution concentration, incubation time and the like. The established indirect ELISA detection method does not have cross reaction with other bovine pathogens, the specificity is good, the detection accuracy is high, and the total coincidence rate of test results of intradermal allergy with bovine tubercle bacillus is 95.8%; the intra-batch variation coefficient of the detected samples is 2.43%-3.61%, the inter-batch variation coefficient is 2.83%-3.96%, and the intra-batch variation coefficient and the inter-batch variation coefficient are both smaller than 10%. A new diagnosis target is developed, the established indirect ELISA method has the characteristics of high sensitivity, strong specificity, good repeatability and the like, the operation is easy, and a new method is provided for the detection work of the mycobacterium bovis.

The invention discloses a method for improving stability of a tuberculin BCG-PPD skin test diagnostic reagent. Twain is adopted for dispersing BCG-PPD stoste, and the volume of the adopted Twain usedfor dispersing is 0.8%-6% of that of the BCG-PPD stoste. The invention further provides a preparation method for a PPD in-vivo diagnostic reagent containing a mycobacterium tuberculosis vaccine BCG component. A reagent combination is high in sensitivity of detecting tuberculosis and has good physical and chemical stability, a wide market prospect and application value. According to the tuberculinBCG-PPD skin test diagnostic reagent prepared by adopting the method, the validity period can be prolonged to 36 months.

The invention provides a bovine tuberculosis serological diagnostic marker and clinical application thereof. Serum markers, namely KLK12, MMP-1 and MMP-9, capable of identifying negative and positive bovine tuberculosis are obtained through transcriptome sequencing analysis, mouse animal experiments and clinical tests. The bovine tuberculosis serological diagnostic marker provided by the invention has the advantages that the diagnostic result of the bovine tuberculosis serological diagnostic marker is 100% consistent with that of a tubercle bacillus skin test, and the bovine tuberculosis serological diagnostic marker can identify and diagnose latent infected cattle and active tuberculosis cattle, is not influenced by infection of other mycobacteria, and has relatively strong specificity and relatively high sensitivity. The diagnostic marker provides a new target spot for diagnosis of bovine tuberculosis, can be used for preparing a serological diagnostic kit for bovine tuberculosis, does not need to collect anticoagulant and specific antigen stimulation, simplifies operation requirements, improves diagnosis efficiency, is beneficial to batch and rapid detection and elimination of tuberculosis cattle, has a wide application prospect, and provides a technical support for prevention, control and purification of bovine tuberculosis.

The invention provides a method for detecting and discriminating the latent tuberculosis infection state, and a kit thereof. The contents of characteristic genes can be quantitatively detected through a real-time quantitative fluorescence amplification method, and the latent tuberculosis state is determined through comparing the multiple of a difference between an expression amount with the use of the tuberculin characteristic genes and an expression amount without the use of the tuberculin characteristic genes.

The invention discloses a production method of tuberculin pure protein derivatives, belonging to the technical field of biological protein preparation. The attenuated Mycobacterium tuberculosis strain ATCC25177 and / or CMCC93020 is used as the strain, and the finished product of tuberculin pure protein derivative is obtained through amplification, inactivation, salting out and sedimentation, sterilization and filtration, inspection, dilution and subpackaging. Solve the defects in the prior art that the strains are harmful to operators and the environment, and require high production conditions (P3 production workshop), and finally obtain a strain that can be used for clinical diagnosis of tuberculosis, screening of BCG vaccination targets, and body immune response after BCG vaccination Proteins used in surveillance and epidemiological surveillance.

The invention relates to a method for measuring bovine tuberculin and activity thereof with high performance liquid chromatography. The purified bovine tuberculin is measured with the high performance liquid chromatography, thus the nucleic acid can be completely separated from the tuberculosis protein, the influence of the nucleic acid to the tuberculosis protein can be completely eliminated, and the relative content of the nucleic acid and the tuberculosis protein can be accurately reflected. The valence of the purified bovine tuberculin is currently calculated with the guinea-pig calibration method which is complex to test and operate, can be affected by many factors and has the large measuring result error. The method can easily and directly measure the valence of the purified bovine tuberculin, is easy to operate and can ensure the accurate and reliable data.

The invention relates to the field of medical test, in particular to a tuberculous infection diagnostic kit, a screening system and application of the kit. The kit contains mitogen and an antigen irritant. The antigen irritant is selected from the following tuberculin pure protein or three or more kinds of derivatives thereof, such as ESAT6, CFP10, TB7.7, MPT64, CFP21, EsxV, Rv1985C and Rv0222. The novel skin experiment for screening latent tuberculosis infection and active tuberculosis, provided by the invention, has the advantages of high specificity (identification of BCG vaccination and tuberculosis infection), high sensitivity (limited multiple antigen protein costimulation), small influence from individual basic immune differences and the like.

The invention discloses a tuberculin micro-needle patch as well as a preparation method and application thereof. The tuberculin micro-needle patch is prepared by the following steps: adding a micro-needle raw material solution, which is prepared by mixing tuberculin, a degradable material and sterile water, into a micro-needle template, wherein the concentration of the tuberculin is 20IU / ml to 200IU / ml and the mass percentage of the degradable material is 10 percent to 20 percent; after carrying out centrifuging treatment, removing part of the raw material on a needle hole of a micro-needle; adding a degradable material solution with the concentration of 5 percent to 10 percent; carrying out the centrifuging treatment again; putting the mixture into a drying oven and drying; then de-molding. The tuberculin micro-needle patch disclosed by the invention can be adhered to an adhesive tape and is directly stuck to skin through the adhesive tape so as to replace traditional injector intradermal vaccination; the total content of the tuberculin in the micro-needle patch is 0.05IU. The inoculation operation is simple and convenient and professional persons are not needed; the dosage of medicines is few and a phenomenon that bad skin response is easily generated by an injection skin test is avoided.

The utility model relates to the technical field of medical teaching molds, and particularly discloses a teaching mold for tubercle bacillus skin test teaching, which comprises a shell and a induration simulation assembly, the shell is at least provided with a flexible diaphragm capable of covering the induration simulation assembly on the simulation working face, the flexible diaphragm is provided with a transparent area and measuring scales, and the displayable area of the transparent area is larger than the maximum size simulation induration on the induration simulation assembly; the hardening simulation assembly is movably assembled on one side of the flexible diaphragm, and the hardening simulation assembly can relatively displace under the action of external force by taking the transparent area on the flexible diaphragm as a reference; the hardening simulation assembly is provided with a substrate and a plurality of simulation hardening parts, the simulation hardening parts are fixed to the simulation working face of the substrate at intervals, and the interval arrangement track of the simulation hardening parts on the substrate corresponds to the displacement direction. The practical operation teaching of the system does not depend on actual tuberculosis infection detection personnel, the teaching process is basically not limited, and the system is flexible and practical.

The invention relates to a method for measuring bovine tuberculin and activity thereof with high performance liquid chromatography. The purified bovine tuberculin is measured with the high performance liquid chromatography, thus the nucleic acid can be completely separated from the tuberculosis protein, the influence of the nucleic acid to the tuberculosis protein can be completely eliminated, and the relative content of the nucleic acid and the tuberculosis protein can be accurately reflected. The valence of the purified bovine tuberculin is currently calculated with the guinea-pig calibration method which is complex to test and operate, can be affected by many factors and has the large measuring result error. The method can easily and directly measure the valence of the purified bovine tuberculin, is easy to operate and can ensure the accurate and reliable data.

The present disclosure provides a use of a fusion protein EH containing a tuberculous allergen or a pharmaceutical composition containing the fusion protein EH in the preparation of a reagent or kit for immunological diagnosis of tuberculosis, auxiliary diagnosis of tuberculosis or screening of latent tuberculosis infection of Mycobacterium tuberculosis, the fusion protein EH containing ESAT6 and HspX. The recombinant allergen EH provided by the present disclosure has good specificity and high sensitivity, can be suitable for large-scale screening, and is a new generation of LTBI screening and diagnosis product technology with application and development potential. The recombinant allergen EH provided by the present disclosure has lower toxicity compared with EC, and is safer.

The invention discloses application of a traditional Chinese medicine composition in preparation of anti-tuberculin medicine. The traditional Chinese medicine composition is prepared by 18 parts of hairyvein agrimony roots, 15 parts of frog orchid tuber, 20 parts of common aeschynomene leaf flower, 12 parts of litsea glutinosa and 20 parts of floscopa scandens. The traditional Chinese medicine composition has remarkable effect of inhibiting activity of tuberculin and meanwhile brings remarkable progress on prevention and control of tuberculin injection.

The invention relates to a differential diagnosis method for tuberculin intradermal allergy-positive cows, in particular to a differential diagnosis method for tuberculin intradermal allergy-positivecows that were last monitored for more than 6 months. It is characterized by the detection method of the Diagnostic Technology of Animal Tuberculosis (GB / T18645-2002). Detection (referred to as ''first monitoring'') is carried out to cows that were last monitored for more than 6 months, and the cows with positive tuberculosis are kept in isolation. A second monitoring is performed within one weekfrom the day of Observation of Skin Thickness Difference at 72 hours after the first monitoring and intradermal injection of tuberculin, and it was found that about 10% of the cows were negative for tuberculosis. A third monitoring is performed at another 45 days' interval and the cows are confirmed to be negative for tuberculosis. The invention reduces the misjudgment and mistake kill rate of tuberculin intradermal allergy test positive cows by about 10%, reduces the losses of dairy farming companies (fields and households) to a minimum and saves financial killing subsidies and working expenses. It is a supplement, improvement and promotion of the Diagnostic Technology of Animal Tuberculosis (GB / T 18645-2002).

The utility model discloses a tubercle bacillus skin test and novel tubercle bacillus skin test integrated measuring scale relates to tubercle bacillus skin test equipment technical field, it includes fixed scale plate and slide scale plate, fixed scale plate is transparent rectangle soft scale plate, fixed scale plate both ends are provided with cross scale mark layer respectively, and slide scale plate is fixed scale plate. A concentric circle pattern layer is arranged on the cross scale line pattern layer located on the left side, a detection ring is arranged on the cross scale line pattern layer located on the right side, the middle of the front face of the fixed ruler plate is concaved inwards to form a hollowed-out groove, and the hollowed-out groove is formed between the two cross scale line pattern layers; the sliding ruler plate is a non-transparent rectangular flexible ruler plate, the sliding ruler plate is arranged in the middle of the front face of the fixed ruler plate in a sliding mode, a buckle is arranged in the middle of the sliding ruler plate, and the buckle can slide in the hollowed-out groove. The utility model can solve the problem that the judgment of the infection state of a detected person is influenced due to the wrong cross use of two measuring rulers.

The invention discloses a bovine tuberculosis gamma-interferon ELISA (enzyme-linked immunosorbent assay) detection kit and application thereof. The kit contains a stimulating protein for detecting bovine tuberculosis and a monoclonal antibody for resisting bovine gamma-interferon; wherein the stimulating protein for bovine tuberculosis detection is an ESAT-6-CFP-10 recombinant protein, and is obtained by connecting an optimized mycobacterium tuberculosis ESAT-6 antigen gene and an optimized mycobacterium tuberculosis CFP-10 antigen gene in series, inserting a prokaryotic expression vector and then performing prokaryotic expression; the monoclonal antibody for resisting the bovine gamma-interferon is secreted by a hybridoma cell with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.45834. Compared with tubercle bacillus, the sensitivity of the ESAT-6-CFP-10 recombinant protein for stimulating T lymphocytes to secrete gamma-interferon is higher than that of bovine tubercle bacillus derivatives, interference of tuberculosis bacteria in the external environment can be eliminated, and the diagnosis accuracy is improved.

The invention discloses a production process of a high-titer tuberculin skin test diagnostic reagent, including bacterial culture, inactivation and precipitation; the specific process of the bacterial culture is: the working seed bacteria are first cultivated and developed into dry wrinkled agglomerates and light yellow Microscopic examination of the lawn showed short and thick bacilli with slightly curved ends and rounded ends; then cultured for 2 generations until the bacterial film was wrinkled and yellowish, and passed on when the bacterial film covered the surface of the medium and the culture medium was clear and transparent Carry out 3 generations of culture, and carry out 4 generations of culture 8-10 weeks after 3 generations of culture until the formation of wrinkled yellowish bacterial film. The present invention provides a production process for high-titer PPD enriched with two antigens, ESAT-6 and CFP10. The PPD prepared by the process of the present invention has high titer, and the content of ESAT-6 and CFP10 is high.

The present invention relates to the application of corimycin in anti-tuberculosis infection, the main steps of which are to use the clinical first-line anti-tuberculosis drugs isoniazid and rifamycin as contrasts, and use the absolute concentration method to carry out the anti-tuberculosis analysis of corimycin. Mycobacterial activity assay, the results show that the activity of carrimycin against clinically isolated Mycobacterium tuberculosis, including drug-resistant bacteria, is significantly better than that of the first-line clinical reference drugs isoniazid and rifamycin, and it is expected to discover the activity of carrimycin New application in the treatment of tuberculosis infection.

The invention relates to the field of medicines, in particular to application of mycocidin in preparation of a bacteriostatic agent for mycobacterium abscessus and / or mycobacterium tuberculosis. The invention firstly discloses the application of the mycocidin in preparation of the bacteriostatic agent for the mycobacterium abscessus and / or the mycobacterium tuberculosis, and further discloses the bacteriostatic agent for inhibiting the mycobacterium abscessus and / or the mycobacterium tuberculosis, and the active ingredient of the bacteriostatic agent is the mycocidin. The mycocide has good bactericidal activity when independently acting on mycobacterium abscessus and / or independently acting on mycobacterium tuberculosis, can be used for treating the tuberculosis, has a remarkable inhibition effect on the mycobacterium abscessus and / or the mycobacterium tuberculosis, and has important significance in preventing and treating the tuberculosis.

The invention relates to bovine type tuberculin standard substance and a preparation method thereof. The bovine type tuberculin standard substance is prepared by the following steps of: preparing bovine type tuberculin through processes of culturing, inactivating, filtering, purifying, and the like by utilizing mycobacteriumbovis separated in China; measuring the contents of protein, nucleic acidand polysaccharide in the bovine type tuberculin; and preparing the bovine type tuberculin standard substance by combining biological test measurement.

The invention discloses a method for improving stability of a tuberculin BCG-PPD skin test diagnostic reagent. Twain is adopted for dispersing BCG-PPD stoste, and the volume of the adopted Twain usedfor dispersing is 0.8%-6% of that of the BCG-PPD stoste. The invention further provides a preparation method for a PPD in-vivo diagnostic reagent containing a mycobacterium tuberculosis vaccine BCG component. A reagent combination is high in sensitivity of detecting tuberculosis and has good physical and chemical stability, a wide market prospect and application value. According to the tuberculinBCG-PPD skin test diagnostic reagent prepared by adopting the method, the validity period can be prolonged to 36 months.

The invention discloses delayed-type allergic reaction antigen epitope polypeptides of mycobacterium bovis CFP-10 and an application thereof. With utilization of an overlapped short peptide piecewise expression technology, identification of the delayed-type allergic reaction antigen peptides is carried out on the CFP-10, and antigen peptides C-PC and C-PB7 having delayed-type allergic reaction are finally identified. The identified antigen peptides C-PC and C-PB7 are connected with M-P11 through Linker by means of genetic engineering, after genetic optimization, a tandem peptide gene CCM is obtained, and simultaneously, a multicopy tandem peptide gene containing multiple CCMs is constructed. The identified antigen peptides and the tandem peptides are subjected to delayed-type allergic reaction identification, and a result show that the C-PB7, the C-PC, the CCM and the 2CCM all can produce relatively strong delayed-type allergic reaction. A problem of poor PPD specificity in a tuberculin skin test (TST) diagnosis is improved, and in addition, the diagnostic sensitivity is increased by increasing the types of the tandem peptides. Therefore, new ideas are put forward for development of bovine tuberculosis skin test diagnostic preparations, and a good application prospect is provided.