Coli bacillus based tubercle bacillus expression library and its uses

A technology for Escherichia coli and Mycobacterium tuberculosis, applied in the field of medicine and biology, can solve the problems of accelerating the revealing of the pathogenic mechanism of Mycobacterium tuberculosis, disadvantages, limitations, etc.

Inactive Publication Date: 2007-07-11
SOUTHWEST UNIVERSITY
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Problems solved by technology

This restricts routine laboratories to conduct research on tuberculosis pathogenic functional genes, which is not conducive to accelerating the discovery of tuberculosis pathogenic mechanisms and the development of new measures to control tuberculosis

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  • Coli bacillus based tubercle bacillus expression library and its uses

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Embodiment Construction

[0021] 1. Materials and Methods

[0022] 1.1 Materials

[0023] 1.1.1 Strains and plasmids: The inactivated Mycobacterium tuberculosis drug-resistant strain 580 was purchased from Chongqing Pulmonary Hospital. The vectors used in the experiment were pUC18, pET32a(+), Escherichia coli JM109, DH5a and BL21(DE3), and lung cancer cell lines A549 is preserved by this laboratory.

[0024] 1.1.2 Main reagents and instruments: various restriction endonucleases, calf intestinal alkaline phosphatase CIAP, T4DNA ligase, and gel recovery kit were purchased from Dalian Bao Biology (TaKaRa) company; RNase, proteinase K, lysozyme , Ampicillin, SDS is a product of Shanghai Sangong. fu DNA polymerase, dNTP, and IPTG were purchased from Beijing Dingguo Biotechnology Co., Ltd. Other reagents were of domestic analytical grade. Primers were synthesized by Shanghai Yingjun Company, and DNA sequencing was completed by Shanghai Boya Biotechnology Co., Ltd. Fatty acid analysis was performed by a ...

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Abstract

The invention discloses a sieved tuberculin functional gene Rv0621 based on escherichia coli, which purifies and recombines Rv0621 to modulate the molecule of surface activity proof agent.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a Mycobacterium tuberculosis expression library based on Escherichia coli and its application. Background technique [0002] Mycobacterium tuberculosis (Mycobacterium tuberculosis, hereinafter referred to as Mycobacterium tuberculosis) has always been one of the main pathogenic microorganisms threatening human health, and its pathogenic mechanism has not yet been fully understood. The completion of the whole genome sequencing of MTB H37Rv and CDC1551 strains in 1998 brought the research on this pathogenic microorganism to a new level, but among the more than 4,000 genes obtained from the whole genome sequence analysis, 16% of the genes Function is completely unknown. Understanding the functions of these genes will provide new clues for in-depth study of the pathogenic mechanism of MTB, and then provide possible targets for the development of new drugs. [0003] When ...

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N15/31C12N1/21C12R1/19
Inventor谢建平廖丹郭茹珍王洪海
OwnerSOUTHWEST UNIVERSITY