Differential diagnosis method for tuberculin intradermal allergy-positive cows

Abstract

The invention relates to a differential diagnosis method for tuberculin intradermal allergy-positive cows, in particular to a differential diagnosis method for tuberculin intradermal allergy-positivecows that were last monitored for more than 6 months. It is characterized by the detection method of the Diagnostic Technology of Animal Tuberculosis (GB / T18645-2002). Detection (referred to as ''first monitoring'') is carried out to cows that were last monitored for more than 6 months, and the cows with positive tuberculosis are kept in isolation. A second monitoring is performed within one weekfrom the day of Observation of Skin Thickness Difference at 72 hours after the first monitoring and intradermal injection of tuberculin, and it was found that about 10% of the cows were negative for tuberculosis. A third monitoring is performed at another 45 days' interval and the cows are confirmed to be negative for tuberculosis. The invention reduces the misjudgment and mistake kill rate of tuberculin intradermal allergy test positive cows by about 10%, reduces the losses of dairy farming companies (fields and households) to a minimum and saves financial killing subsidies and working expenses. It is a supplement, improvement and promotion of the Diagnostic Technology of Animal Tuberculosis (GB / T 18645-2002).

Description

Technical field: [0001] The invention relates to a differential diagnosis method for tuberculin intradermal allergy-positive dairy cows, in particular to a differential diagnosis method for tuberculin intradermal allergy-positive dairy cows at an interval of more than 6 months from the last monitoring. Background technique: [0002] The current diagnostic methods for live bovine tuberculosis include: disease material acid-fast staining microscope, pathogen isolation and culture, animal inoculation test, PCR identification, γ-interferon enzyme-linked immunosorbent assay and Mycobacterium bovis PPD (purified protein derivative) Intradermal allergy test (hereinafter referred to as "tuberculin intradermal allergy test"), etc. Microscopic examination of acid-fast staining of disease materials, pathogen isolation and culture, animal inoculation test, PCR identification and gamma-interferon enzyme-linked immunosorbent assay have the advantages of strong specificity, but there are a...

AI Technical Summary

Problems solved by technology

Microscopic examination of acid-fast staining of disease materials, pathogen isolation and culture, animal inoculation test, PCR identification and gamma-interferon enzyme-linked immunosorbent assay have the advantages of strong specificity, but there are also low positive detection rates (that is, high missed detection rate) ), infectious occupational exposure risks, and high requirements for facilities and equipment; tuberculin intradermal allergy test is currently a world-recognized method for diagnosing (monitoring) tuberculosis in live cattle, and many countries in the West use this method to monitor live cattle for tuberculosis Combined with killing and harmless treatment of tuberculosis-positive cattle, disinfection and other comprehensive measures have extinguished bovine tuberculosis in China. This method is also a recommended method for the diagnosis of live bovine tuberculosis in my country, with high detection rate, low risk of infectious occupational exposure and The advantages of low requirements for facilities and equipment, but also the disadvantage of poor specificity (false positives)