Bovine type tuberculin standard substance and preparation method thereof

A technology of tuberculin and standard substance, which is applied in the field of bovine tuberculin standard substance and its preparation, and can solve the problem of high quality requirements

Inactive Publication Date: 2012-10-31
CHINA INST OF VETERINARY DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quality requirements of standard products are high, and the preparation methods and inspection or calibration methods are strict. There are no relevant reports on the preparation procedures and calibration methods.

Method used

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  • Bovine type tuberculin standard substance and preparation method thereof
  • Bovine type tuberculin standard substance and preparation method thereof
  • Bovine type tuberculin standard substance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1 Preparation of bovine type purified tuberculin national standard material

[0104] 1. Medium

[0105] Sutong Synthetic Medium (Regulations Committee for Veterinary Biological Products of the Ministry of Agriculture. Regulations for Veterinary Biological Products of the People’s Republic of China, 2000 Edition, Chemical Industry Press, 2000) The ingredients include the following, asparagine 4g, citric acid 2g, magnesium sulfate 0.5 g. Dipotassium hydrogen phosphate 0.5g, ferric ammonium citrate 0.05g, glycerin 60ml, each component is analytically pure. Add 940ml of distilled water to prepare a total of 50000ml.

[0106] 2. Bacteria

[0107] Mycobacterium bovis C68001 and C68002 strains (provided by China Veterinary Drug Inspection Institute).

[0108] (1) Preparation of seeds for production

[0109] 1) Primary seed preparation

[0110] Inoculation After opening the freeze-dried strains, inoculate Xiaochuan medium and place them in a greenhouse at 37°C for about two weeks...

Embodiment 2

[0137] Example 2 Finished product inspection of bovine type purified tuberculin national standard material

[0138] 1. Uniformity test Take 15 samples, use Kjeldahl method to determine the protein content of tuberculin in each ampoule, see Table 3.

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Abstract

The invention relates to bovine type tuberculin standard substance and a preparation method thereof. The bovine type tuberculin standard substance is prepared by the following steps of: preparing bovine type tuberculin through processes of culturing, inactivating, filtering, purifying, and the like by utilizing mycobacteriumbovis separated in China; measuring the contents of protein, nucleic acidand polysaccharide in the bovine type tuberculin; and preparing the bovine type tuberculin standard substance by combining biological test measurement.

Description

Technical field [0001] The invention relates to a bovine tuberculin standard substance and a preparation method thereof, and belongs to the technical fields of standard substance technology and veterinary biological products. Background technique [0002] Bovine tuberculosis is a chronic bacterial disease of animals and humans caused by Mycobacterium bovis. The disease has a worldwide distribution. It is also the main infectious disease of cattle in our country. It can be transmitted to humans and cause people to suffer from tuberculosis. The health threat is greater. As my country implements the elimination system for bovine tuberculosis-positive cattle, bovine tuberculosis diagnostic technology is particularly important. Bovine tuberculin diagnostic technology is the only diagnostic method recommended by the World Organization for Animal Health (OIE). The titer calibration of the purified bovine tuberculin product is based on the skin allergy method of guinea pigs. The standar...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A61K49/00
Inventor关孚时林朝洪王在时申咏红肖朝云李翠戴志红张秀英陆连寿蒋卉
OwnerCHINA INST OF VETERINARY DRUG CONTROL