A method for improving the stability of tuberculin bcg-ppd skin test diagnostic reagent
A diagnostic reagent, tuberculin technology, applied in the field of improving the stability of tuberculin BCG-PPD skin test diagnostic reagent, can solve the problems of short product shelf life, can not meet the actual market demand, etc., to achieve good stability and broad market Prospect and application value, effect of good physical and chemical stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0051] Example 1 bacterial culture
[0052] (1) 1 seed of recovery work was inoculated into 5 Roche's egg culture medium, and cultured for 2 weeks. After the bacteria grew into dry, wrinkled and slightly light yellow bacterial film, the growth of the bacterial lawn could be observed at this time: the end of the culture After that, each 1st-generation strain was first suspended with 5ml of sodium chloride 0.9% solution, collected all the bacterial liquid, and sampled for acid-fast staining. Acid-fast staining was positive (bacteria were stained red). Biochemical reaction: Nitrate reduction reaction is positive, resulting in red; urease reaction is positive, showing red; heat-resistant catalase reaction should be negative, no bubbles are generated in 10-20 minutes; polysorbate 80 hydrolysis reaction does not change color. Each medium tube was resuspended with 2ml of physiological saline to prepare a bacterial solution, and a total of 10ml (1st generation) was collected. The bac...
Embodiment 3
[0066] Example 3 Precipitation
[0067] A 40% trichloroacetic acid solution was prepared, added to the harvest solution at a ratio of 1:10, with a final concentration of 4%, and allowed to stand at room temperature for 2 hours. After shaking well, centrifuge at 4°C and 8000rpm for 20min. The precipitate was collected and weighed 450 g.
[0068] Precipitate 450g is dissolved with 3150ml of 0.9% sodium chloride solution, adjusts the pH value with 1M sodium hydroxide, adds ammonium sulfate according to the amount of precipitation dissolution, makes its concentration reach 60% saturation. Add 390g×3.15L=1228.5g of ammonium sulfate, let stand at 2-8°C overnight (16 hours), centrifuge at 8000rpm for 20min at 4°C, and collect 365g of precipitate. Add 2555ml of 0.9% sodium chloride solution to dissolve the precipitate. Add 40% trichloroacetic acid to a final concentration of 4%, precipitate protein, centrifuge at 8000 rpm, collect the precipitate, and weigh 310 g. The precipitatio...
Embodiment 5
[0071] Embodiment 5 sterile filtration
[0072] The dialysate was filtered and clarified through a 1.2μm-0.45μm microporous membrane. After filtration, a 10ml sample was taken to determine the microbial limit, and then sterilized and filtered through a 0.45μm-0.22μm filter membrane to obtain the stock solution. A total of 1800ml was harvested. The sterilization and filtration time should be controlled within 4 hours. The stock solution was prepared according to the protein concentration of 9mg / ml / , and then lyophilized for storage.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More