Tuberculous infection diagnostic kit, screening system and application of kit

Abstract

The invention relates to the field of medical test, in particular to a tuberculous infection diagnostic kit, a screening system and application of the kit. The kit contains mitogen and an antigen irritant. The antigen irritant is selected from the following tuberculin pure protein or three or more kinds of derivatives thereof, such as ESAT6, CFP10, TB7.7, MPT64, CFP21, EsxV, Rv1985C and Rv0222. The novel skin experiment for screening latent tuberculosis infection and active tuberculosis, provided by the invention, has the advantages of high specificity (identification of BCG vaccination and tuberculosis infection), high sensitivity (limited multiple antigen protein costimulation), small influence from individual basic immune differences and the like.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a diagnostic kit for tuberculosis infection, a screening system and the application of the kit. Background technique [0002] At present, about 1 / 3 of the world's population is infected with Mycobacterium tuberculosis, and only 5-10% of them develop into contagious active tuberculosis patients. Therefore, how to quickly and easily find active tuberculosis patients among latent infections appears to be particularly important. In China and other countries where newborns are vaccinated with BCG, since the genome similarity between BCG and Mycobacterium tuberculosis exceeds 95%, it is first necessary to distinguish latent infections from vaccine recipients in reality. The tuberculosis skin test (TST) has the advantage of being economical and simple, and it still has a certain value in use in many countries around the world. TST has undergone a transformation from intradermal inject...

AI Technical Summary

Problems solved by technology

TST has undergone a transformation from intradermal injection of tuberculin (OT) to injection of pure protein derivative of tuberculin (PPD). The specificity of tuberculosis screening has been improved, but it still cannot detect the infection of Mycobacterium tuberculosis. Distinguished from non-tuberculous mycobacterium infection, BCG vaccination, etc.

Therefore, the current TST test still has the disadvantages of insufficient sensitivity and specificity, and the inability to distinguish latent infection from active tuberculosis. In addition, it is also greatly affected by the large difference in immune status between individuals.

Images