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Medium for mold quantity examination and preparation and application thereof

A culture medium and agar medium technology, applied in the field of biological analysis, can solve the problems affecting the accuracy, etc., and achieve the effect of comprehensive mold phase and accurate mold quantity results

Active Publication Date: 2013-02-27
SHANGHAI TOBACCO GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it can affect the accuracy of the test results of the number of molds in the sample to be tested

Method used

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  • Medium for mold quantity examination and preparation and application thereof
  • Medium for mold quantity examination and preparation and application thereof
  • Medium for mold quantity examination and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Prepare medium according to the following formula:

[0039] Potatoes 200g

[0040] Glucose 20.0g

[0041] NaCl30.0g

[0042] Agar 16.0g

[0043] Chloramphenicol 0.1g

[0044] Make up 1000mL with distilled water. Medium preparation method: Peel and cut potatoes into pieces, add 1000mL distilled water, and boil for 10min. Filter with gauze, add glucose, NaCl and agar, add distilled water to 1000mL, heat to dissolve, and then sterilize at 121°C for 20min. Before pouring the plate, dissolve chloramphenicol with a small amount of ethanol and add it to the medium.

Embodiment 2

[0046] Prepare medium according to the following formula:

[0047] Potatoes 300g

[0048] Glucose 15.0g

[0049] NaCl25.0g

[0050] Agar 25.0g

[0051] Make up 1000mL with distilled water.

[0052] Medium preparation method: Peel and cut potatoes into pieces, add 1000mL distilled water, and boil for 10min. Filter with gauze, add glucose, NaCl and agar, add distilled water to 1000mL, heat to melt, after aliquoting, sterilize at 121°C for 20min, pour plate.

Embodiment 3

[0054] Prepare medium according to the following formula:

[0055] Potatoes 200g

[0056] Glucose 25.0g

[0057] NaCl35.0g

[0058] Agar 15.0g

[0059] Chloramphenicol 0.1g

[0060] Make up 1000mL with distilled water.

[0061] The medium preparation method is the same as in Example 1.

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Abstract

The present invention discloses a medium for mold quantity examination and application thereof. The medium for mold quantity examination of the present invention is a medium of NaCl added with potato dextrose agar having a concentration of 10-50g / 1000ml. The medium for mold quantity examination does not affect the general mold growth in tobacco and tobacco products, and also meets the needs of growth of the mold with a xerophilous characteristic, and is especially suitable for mold quantity examination in tobacco and tobacco products.

Description

technical field [0001] The invention relates to the technical field of biological analysis, in particular to a culture medium for mold quantity inspection and its preparation and application. Background technique [0002] In the prior art, there are four kinds of culture media commonly used in tobacco and tobacco products for the inspection of the number of molds: Bengal Red Agar Medium, Potato Dextrose Agar Medium, Chapeyer's Agar and High Osmotic Chapeyer's Agar Culture with 20% sucrose base. [0003] The formula of Bengal red agar medium is: peptone 5.0g, glucose 10.0g, potassium dihydrogen phosphate 1.0g, magnesium sulfate (anhydrous) 0.5g, agar 20.0g, Bengal red 0.033g, chloramphenicol 0.1g, distilled water 1000mL . The medium can meet the growth of general molds in the environment, and can also meet the growth of some molds with xerophilic characteristics. However, Aspergillus limitiosa, a common sicca-like contaminating mold in tobacco and tobacco products, cannot ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 林华清沙云菲戚大伟吴达
Owner SHANGHAI TOBACCO GRP CO LTD
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