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DNA (deoxyribonucleic acid) vaccine for expressing infectious bursal disease virus polyprotein gene VP243, as well as construction method and application thereof

A polyprotein gene and DNA vaccine technology, applied in the DNA vaccine and its construction, in the field of DNA vaccines expressing the infectious bursal disease virus polyprotein gene VP243, can solve the immune failure, mutation, failure to solve the IBDV antigen And other issues

Active Publication Date: 2015-02-04
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, attenuated vaccines and inactivated vaccines are mainly used to prevent IBD. With the emergence of IBDV mutant strains and super-virulent strains, immune failure often occurs.
Although the VP2 protein expressed by recombinant subunit vaccines and live vector vaccines can induce neutralizing antibodies to varying degrees, it still fails to solve the problem of IBDV antigenic variation

Method used

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  • DNA (deoxyribonucleic acid) vaccine for expressing infectious bursal disease virus polyprotein gene VP243, as well as construction method and application thereof
  • DNA (deoxyribonucleic acid) vaccine for expressing infectious bursal disease virus polyprotein gene VP243, as well as construction method and application thereof
  • DNA (deoxyribonucleic acid) vaccine for expressing infectious bursal disease virus polyprotein gene VP243, as well as construction method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 2

[0043] The immunoassay of embodiment 2 DNA vaccine of the present invention

[0044] 1. Materials and methods

[0045] 1.1 Reagents

[0046] IBDV antibody detection kit was purchased from IDEXX company.

[0047] 1.2 SPF chicken

[0048] SPF chickens were provided by the Experimental Animal Center of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, and 14-day-old SPF chickens were kept in a negative pressure isolator in the poultry disease infection laboratory during the experiment.

[0049] 1.3 Method

[0050] The 14-day-old SPF chickens were randomly divided into 4 groups, with 10 chickens in each of the first and second groups, and injected with pCAGoptiVP243 and pCAGGS respectively, 100 μg / bird, at two points in the leg muscles. The same dose and route were used 14 days after the first immunization Boost immunity. Group 3 (CC) and Group 4 (NC), 5 chickens each, were not immunized. 14 days after the second immunization, groups 1, 2, and 3 w...

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Abstract

The invention discloses a DNA (deoxyribonucleic acid) vaccine for expressing infectious bursal disease virus polyprotein gene VP243, as well as a construction method and application thereof. A polyprotein gene (VP243) of an infectious bursal disease virus virulent strain (HLJ0504) is optimized by chicken bias codons, and cloned to a eukaryotic expression vector pCAGGS so as to construct a recombinant expression vector pCAGoptiVP243. An initially immunized 14-day SPF chick is injected with 100mu g of the prepared pCAGoptiVP243 though two points of crureus in, a 28-day chick is subjected to the secondary immunization in same dosage and way, and IBDV (infectious bursal disease virus) virulent strain (HLJ0504) strain is attacked in 14 days after secondary immunization. Results indicate that the DNA vaccine can be used for inducing antibody with higher level and providing immunized protection to the virulent strain.

Description

technical field [0001] The invention relates to a DNA vaccine and its construction method and application, in particular to a DNA vaccine expressing infectious bursal disease virus polyprotein gene VP243 and its construction method and application. The invention belongs to the field of biomedicine. Background technique [0002] Infectious Bursal Disease Virus (IBDV) mainly infects central immune organs such as chicks' bursal disease, leading to immunosuppressive diseases characterized by lymphocyte exhaustion. IBDV is a double-stranded, double-segment RNA virus that belongs to the genus Avian BiRNAvirus of the family DiRNAviridae. Its genome consists of two segments, A and B, and the B segment (about 2.8 kb) encodes the VP1 protein, which is RNA-dependent. RNA polymerase. The A segment (about 3.3kb) has two partially overlapping open reading frames (ORFs). ORF1 encodes a precursor fusion protein (VP243) with a molecular weight of about 110kDa. The precursor fusion protein ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P31/14
Inventor 王笑梅高宏雷高玉龙祁小乐秦立廷王永强
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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