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Intracellular dialysis apparatus and dialysis method thereof

A dialysis device and cell technology, which is applied in the field of medical devices, can solve the problems of low replacement efficiency, difficult operation, and high noise, and achieve the effects of high fluid exchange efficiency, stable observation, and easy operation

Active Publication Date: 2013-07-10
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early intracellular perfusion devices have complex structures, high noise, difficult operation, and low experimental success rate, making it difficult to popularize in general electrophysiological laboratories
Although it has been improved in the later period, it still has the disadvantages of low replacement efficiency and poor stability.

Method used

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  • Intracellular dialysis apparatus and dialysis method thereof

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Embodiment Construction

[0021] The present invention is described in further detail below in conjunction with accompanying drawing:

[0022] see figure 1 and figure 2 , an intracellular dialysis device, comprising an electrode dialysis unit and an electrode holder, the electrode dialysis unit is provided with a first microcannula and a second microcannula, the electrode holder is provided with a central lumen, and the electrode holder The device is provided with a negative pressure suction side tube and a microcannula sleeve communicating with the central lumen, and the first microcannula and the second microcannula are simultaneously inserted into the microcannula sleeve and communicated with the central lumen.

[0023] The electrode dialysis unit includes a liquid storage tank, a U-shaped tube, a waste liquid collection tank, a first micro-cannula, a second micro-cannula, an electrode holder and a three-hole support; one end of the U-shaped tube passes through a silicone tube It communicates wit...

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Abstract

The invention discloses an intracellular dialysis apparatus and a dialysis method thereof. The apparatus comprises an electrode dialysis unit and an electrode holder, wherein the electrode dialysis unit is provided with a first micro-cannula and a second micro-cannula, inside of the electrode holder is provided with a central pipe cavity, the electrode holder is provided with a negative-pressure suction side pipe and a micro-cannula sleeve in communication with the central pipe cavity, the first micro-cannula and the second micro-cannula are simultaneously inserted into the micro-cannula sleeve and communicated with the central pipe cavity. According to the invention, beneficial effects are that the intracellular dialysis operation are simple, a liquid changing efficiency is high and a long-term stable observation can be carried out.

Description

Technical field: [0001] The invention belongs to the field of medical equipment, and relates to an electrode liquid replacement device, in particular to an intracellular dialysis device and a dialysis method thereof. Background technique: [0002] Patch clamp technology has become more and more mature and perfect since its creation. Using this technology, people can conduct in-depth analysis of the properties and functions of membrane proteins (such as ion channels and receptors) at the molecular level. But in some experiments, the technique has been limited. For example, when studying the regulation of ion channels by the cell membrane signaling system, it is necessary to apply appropriate drugs in the cells; when analyzing and measuring the current mechanism corresponding to a certain functional state of the cell, it is necessary to use the corresponding electrode fluid according to the measured current. In these cases, strict controls can only be achieved by using a spec...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 邢俊玲胡三觉杨晶王文挺段建红
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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