Bacillus thuringiensis cry1Ah/cry1Ie bivalent gene expression vector and application thereof
A m2-cry1ah, m2-cry1ie technology, applied in the biological field, can solve the problems of low protein expression and low transformation efficiency, and achieve the effect of high expression and good application value
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0047] The present invention is described in further detail below in conjunction with embodiment.
[0048] The biological materials used below are preserved in the applicant's laboratory and can be distributed to the public.
[0049] 1. Imported genes:
[0050] Target gene: In this study, the cry1Ah and cry1Ie genes were optimized twice, and the modified cry1Ah gene (m2-cry1Ah) was compared with the original cry1Ah gene: the GC content increased from 37% to 55%. The GC content of the optimized cry1Ie gene (m2-cry1Ie) was increased to 55%. See the sequence listing for the modified nucleotide sequence and deduced amino acid sequence. Table 1 shows the GC content and codon usage frequency of the secondary transformed cry1Ah and cry1Ie genes
[0051] Table 1 GC content and codon usage frequency of transformed cry1Ah and cry1Ie genes
[0052]
[0053]
[0054] 2. Construction of recombinant vector pMAhIeb:
[0055] Using pCAMBIA3300 as the vector backbone, the insert seq...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com