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A research method for dominant male sterility material of buckwheat
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A technology of male sterility and nuclear sterility, applied in the field of naked oat dominant male sterility material research, can solve the problem of no dominant male sterility material found
Inactive Publication Date: 2017-02-08
ZHANGJIAKOU ACAD OF AGRI SCI
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However, there is no report on the use of dominant male sterile materials in naked oats.
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[0050] The materials used in the embodiment of the present invention are divided into two categories, one is: female parental sterility material, and the other is: male parental material for testing and mating.
[0051] 1. Obtaining maternal sterility materials. In 1996, it was discovered and obtained in the naked oats experimental field of the former Bashang Agricultural Science Institute of Zhangjiakou City. The process is as follows: In 1994, two transgenic substances (Pucl8 and Gus) were introduced from the Institute of Botany, Chinese Academy of Sciences, and then transferred to the naked oat variety "Pin No. 5" as two salt-alkali resistance genes. The method is as follows: use a sharp blade to cut off the stigma of naked oat embryos that have been pollinated for 4 hours, drop 5 μl of DNA, and make 500 flowers for each treatment. There are 2 treatments in total, namely P5P and P5G. The seeds harvested in autumn are 95 and 115 respectively. In 1995, it was planted in the...
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Abstract
The invention relates to a researching method of a dominant male genic male sterile material of hulless oat. The researching method specifically comprises the following steps: (1), implementing a characteristic and feature researching method of the sterile material; (2), implementing the research on a hybridization method; (3), implementing a genetic analysis researching method of the sterile material, namely, 1, surveying the fertility of an F1-generation sterile material; 2, tracking and surveying a fertile line separated from the F1-generation sterile material; 3, surveying fertility conditions of the sterile material under different ecological conditions; (4), implementing a sterility surveying method of the sterile material; and (5), implementing application of a genic male sterile material on a hulless oat, namely, 1, hybridization; 2, seed selection; and 3, breeding. By adopting a breeding procedure of a genic male sterile single-cross single-gene progressive summating method, a genic male sterile double-cross double-gene progressive summating method and a dynamic gene pool hybridization breeding method, a research on a recurrent selection method is developed. According to the researching method, the breeding level and the selecting efficiency of the hulless oat are increased, the increase of producing varieties, the high speed of producing the varieties, and the good quality of produced varieties are facilitated, the social benefit is large, the hybridization efficiency is improved, the cost is lowered, and the economic benefit is good.
Description
technical field [0001] The invention relates to a research method for naked oat dominant male sterility material, which belongs to the field of biotechnology. Background technique [0002] Naked oats (A. nuda large grain naked oats) are the main food crops and feed in high latitude, high altitude, alpine and remote mountainous areas such as the three provinces of Hebei, Shanxi and Inner Mongolia in the north of North my country, the four provinces of Shaanxi, Gansu, Ningxia and Qinghai in the northwest, and the Liangshan and Liangshan mountains in southwest Yunnan, Guizhou, and Sichuan. The planting area of forage crops in the alpine mountainous areas of Bashang and Baxia in Zhang and Cheng of our province accounts for 30-50% of the grain sowing area. In recent years, studies at home and abroad have confirmed that its seeds have the effect of lowering blood fat and blood sugar in the human body, and have been developed and utilized as a functional health food. Therefore, i...
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