54results about How to "Clear type" patented technology

The invention discloses a three-generation sequencing platform-based HLA genotyping method. The method comprises the following steps of (1) performing PCR amplification on HLA genes needed to be genotyped; (2) after a PCR product is detected to be qualified, performing three-generation sequencing to obtain original data; (3) performing long sequence comparison on the original data and a referencegene sequence; (4) after comparison, correcting sequencing errors; (5) performing phase splitting to obtain a haplotype sequence; and (6) performing genotyping judgment. Compared with an existing HLAgenotyping method, the HLA genotyping method has the advantages of few requirements on computing resources, quick genotyping and high resolution, and has important values for application and basic research work of clinical transplantation tissue type matching, population genetics, anthropology, evolutiology and the like.

The invention discloses a preparing method of a high-strength and wear-resistant Cu-Ni-Sn alloy coating and belongs to the technical field of metal materials. The preparing method comprises the following steps: first, adopting a gas atomization method to prepare Cu-Ni-Sn-Nb alloy powder; and then, coating the hot alloy powder on the surface of a base body provided with a Ni-cladded aluminum base coat, and after aging treatment, obtaining the high-strength and wear-resistant Cu-Ni-Sn alloy coating attached to the surface of the base body. The high-strength and wear-resistant Cu-Ni-Sn alloy coating can be used for the surface reinforcement of guide rails, bearings, bearing bushes and the like which are used in high-strength severe environments. On the basis of ensuring that the surface of the base body material has the excellent properties of Cu-Ni-Sn alloy, raw materials are greatly saved. The production cycle is shortened, and the production efficiency is increased. The obtained alloy coating has the advantages of being high in strength and great in wear resistance.

The invention relates to the technical field of traditional Chinese medicines and in particular discloses venenum bufonis extract with anti-infection action and a preparation method thereof. The venenum bufonis extract contains a phrynin effective component which contains the following components in parts by weight: 30-60 parts of arenobufagin, 35-60 parts of cinobufagin, 10-25 parts of telocinobufagin, 20-45 parts of bufotaline, 20-50 parts of cinobufotalin, 25-45 parts of bufalin, 15-40 parts of resibufogenin and 5-15 parts of hellebrigenin. The venenum bufonis extract provided by the invention is remarkable in anti-infection effect. In addition, the types and content of effective components of the extract are clear, the cytotoxicity is small, and quality control and medicinal safety of the extract are facilitated.

The invention relates to a preparation method of a homotype heterojunction based semiconductor nitrogen dioxide gas sensitive layer. The method includes: taking oxidation treated monocrystalline silicon wafer Si as the insulation substrate, preparing an interdigitated electrode and a terminal on the front of the insulation substrate, dissolving mixed powder of zinc nitrate and tin chloride in deionized water or an organic solvent, and performing stirring to obtain a homogeneous solution to serve as a precursor, sending the precursor into flame flow generated by a plasma spray gun through a non-atomizing nozzle, carrying out evaporation, decomposition, nucleation, heating and acceleration series reaction, and letting molten particles bombard the position right above the interdigitated electrode so as to obtain a semiconductor gas sensitive coating. The method provided by the invention overcomes the defects of complex steps, long experiment time, low efficiency, basically no repeatability and the like in previous methods. According to the invention, the homotype heterojunction semiconductor gas sensitive layer can realize compounding of a variety of homotype semiconductor materials, and the prepared gas sensitive material has the advantages of high sensitivity to low concentration NO2, fast response speed and the like.

The invention belongs to the technical field of liquor brewing, and provides an acid control and efficiency improvement method for Daqu delicate fragrance liquor in hot season, in order to solve the problems that the liquor produced by using Daqu as a saccharification fermentation agent goes rancidity easily in the hot season, and the liquor yield is affected. A Fen Daqu delicate fragrance Liquor brewage technology is employed, the consumption of Daqu is decreased, and rhizopus distiller's yeast, Saccharomyces cerevisiae and ester producing yeast are in cooperation; and sorghum is used as a raw material for fermentation liquor brewing. The dosage of the yeasts is as below: Daqu accounts for 2.5-7.5% of the raw material, rhizopus distiller's yeast accounts for 0.3-0.5% of the raw material, and Saccharomyces cerevisiae accounts for 0.05-0.01% of the raw material, and the ester producing yeast accounts for 0.05-0.1% of the raw material. The material and yeasts are subjected to ground tank fermentation for 13-15 days; and the initial fermentation temperature is controlled at 25-30 DEG C. The method effectively reduces the amount of Lactobacillus seed, ensures product flavor, presents novel style, greatly reduces the usage amount of the Fen liquor Daqu, lowers costs, and improves the efficiency of liquor brewing in the premise of effective acid control in the hot season. The liquor reserves the aroma function of Fen liquor Daqu, preserves complex composition, and guarantees the flavor characteristics of Fen liquor.

The invention discloses a method for detecting polymorphism of a TREM2 gene, wherein the method includes the following steps: determining a mutation site of the TREM2 gene; designing and synthesizinga probe and primers according to the mutation site; mixing the detected TREM2 target gene, the probe, the primers and enzymes, carrying out fluorescence quantitative PCR, and monitoring a fluorescencesignal; and calculating the Tm value displayed by hybrid peaks, and judging the type of the TREM2 gene according to the Tm value. The invention also discloses the probe for detecting the polymorphismof the TREM2 gene, and the probe has the nucleotide sequence including SEQ ID NO.3. The invention provides the probe and method for simple operation and rapid detection of the polymorphism of the TREM2 gene.

An ultrasonic phased array nondestructive test method based on fractal is disclosed. The method comprises following two parts: Bayesian information fusion algorithm and signal fractal processing. The Bayesian information fusion algorithm includes following steps of: taking each array element of a ultrasonic phased array probe, separately collecting a median, an upper quartile and a lower quartile in signals in sequence to obtain a quartile dispersion, solving an eliminative point to obtain an effective data fusion set, solving characteristic functions and decision functions of effective data to obtain a risk function, and extracting Bayesian risk of the decision functions to obtain optimal estimated values of parameters. The signal fractal processing includes following steps of: analyzing image fractal characteristics through a wavelet decomposition coefficient figure to determine a scale-free zone, calculating a fractal dimension, and determining whether a flaw exists and determining a flaw type according to the fractal dimension. Based on adoption of the Bayesian information fusion algorithm to improve the information utilization rate, the method adopts a fractal technology to process nonlinear modern signals so as to determine whether a flaw exists in a composite material and to determine a flaw type.

The invention provides a probe for detecting the polymorphism of an ADRB1 gene. The sequence of the probe is shown as SEQ ID NO.: 1, or the two ends of the sequence of SEQ ID NO.: 1 are modified by groups. The invention also provides a primer for detecting the polymorphism of the ADRB1 gene. The sequences of the primer are shown as SEQ ID NO.: 2 and SEQ ID NO.: 3. The probe and primer for detecting the polymorphism of the ADRB1 gene have the advantages of high sensitivity, good specificity, rapidness, high-throughput detection and the like when being used for detecting the polymorphism of theADRB1 gene.

The invention relates to a controllable preparation method of a papain-wrapped gold nanocluster, and belongs to the technical field of nanotechnology. Papain is taken as a template, and the influenceof the change of the pH value in the reaction process on the optical performance of the gold nanocluster is explored; and by controlling the reaction pH, the temperature and the time, the gold nanoclusters capable of emitting green, blue and yellow fluorescence with the particle size of approximately equal to 1nm are correspondingly synthesized. The synthesis method is simple and green, and the synthesized gold nanocluster has good optical stability, high anti-light bleaching capability, and high catalytic performance and electroluminescence performance, and can be used as a good photochemistry, electrochemical signal probe or catalyst.

The invention provides a parallel chain self-consensus method and device and a storage medium, and the method comprises the steps: synchronizing each parallel chain transaction of a parallel chain from a main chain block, generating a first parallel chain block and a corresponding first consensus transaction if the parallel chain transaction of a non-self-consensus transaction does not exist in each parallel chain transaction, and not sending the first consensus transaction temporarily; if the parallel chain transaction of the non-self-consensus transaction exists in the parallel chain transactions, executing the transaction, generating a second parallel chain block and a second consensus transaction of the second parallel chain block; if a plurality of first consensus transactions which are not sent temporarily exist, packaging the plurality of first consensus transactions which are not sent temporarily and the second consensus transaction into a first transaction group, and sending the first transaction group to the main chain node; when a plurality of first consensus transactions which are not temporarily sent do not exist, sending the second consensus transactions to the main chain node; the number of empty blocks is effectively reduced.

The invention discloses a two-stage sliding block device for a die and belongs to the technical field of dies. The two-stage sliding block device comprises a base plate, wherein a primary sliding block is arranged on the base plate through a limiting sliding component; a wedge-shaped cavity is formed in the primary sliding block; the upper wall of the wedge-shaped cavity is a slope; an opening is formed in the lower part of the wedge-shaped cavity; a secondary sliding block is arranged in the wedge-shaped cavity; the upper part of the secondary sliding block is an inclined neck part matched with the wedge-shaped cavity; the inclined neck part is connected with the wedge-shaped cavity through a guide sliding chute component in a sliding manner; the outer end face of the inclined neck part forms a cavity surface I; a cavity I is formed in the lower part of the outer end of the inclined neck part; a perpendicular part is arranged at the lower part of the inner end of the inclined neck part; the lower end of the perpendicular part is in sliding contact with the base plate; a positioning slot is formed in the base plate. The two-stage sliding block device is applied to the die which needs to be subjected to lateral parting as well as upward parting and downward parting at the same time; the complicated parting problem is solved with a simple structure; the two-stage sliding block device is accurate in positioning and clear in parting and can guarantee the precision and improve the production efficiency.

The invention discloses a composite edible fungus polysaccharide preparation with effects of immunizing and regulating intestinal flora as well as a preparation method and application thereof, and belongs to the technical field of health foods. The invention aims to provide a composite edible fungus polysaccharide preparation capable of regulating intestinal flora and improving immunity as well asa preparation method and application of the composite edible fungus polysaccharide preparation. The composite edible fungus polysaccharide preparation is prepared from the following raw materials inparts by weight: 20-40 parts of edible fungus polysaccharide and 20-40 parts of starch. The preparation method comprises the following steps: crushing 20-27 parts of agaricus blazei murill, 10-17 parts of grifola frondosa, 45-53 parts of hericium erinaceus, 36-45 parts of shiitake mushrooms, 35-40 parts of poria cocos, 12-18 parts of ginseng, 3000 parts of water and food-acceptable auxiliary materials, adding water, extracting, filtering and concentrating an extracting solution, adding auxiliary materials, filtering and sterilizing. The product provided by the invention is good in safety, andclear in regulation of intestinal flora portal level and family level variety, and provides a good thought for research, development and production of related foods and medicines.

The invention discloses a PARMS marker based on a rice brown planthopper resistance gene Bph14 and application of the PARMS marker, and particularly relates to a functional PARMS marker based on rice brown planthopper resistance gene Bph14 first exon SNP non-synonymous mutation and application of the functional PARMS marker. According to the invention, first-generation sequencing is utilized to detect the difference of Bph14 in a high brown planthopper resistance material Y009 and a susceptible brown planthopper variety Nipponbare, T->C mutation occurs at the 448bp site of the first exon of the gene to cause the change of phenylalanine into leucine so as to cause no function of Bph14, the difference of the two basic groups is introduced into a designed forward primer, and two different fluorescence labeling universal primers are added on the basis, so that the fluorescence functional molecular marker of the rice brown planthopper resistance gene Bph14 based on the PARMS technology is developed, and the application of the Bph14 gene in rice resistance molecular breeding is facilitated.

The invention belongs to the technical field of nursing, and discloses a cleaning system for female reproduction nursing and a method. The system comprises a regular reminding module, an image acquisition module, a master control module, an image processing module, a lighting module, an injection module and a display module. The regular reminding module can provide a regular reminding function, regular cleaning of a user is facilitated, regular reminding time is calculated according to regular initial time and a regular period, reminding is carried out when the regular reminding time is reached, thus, reminding is carried out every other fixed time, and the flexibility of regular reminding of a cleaning device for female reproduction nursing is improved. Meanwhile, by means of the image processing module, the labor intensity of clinic workers can be relieved, the culture period of staff is shortened, subjective errors are reduced, and vaginal microecosystem detection is objective, systematic and standardized.

The invention discloses a metallic travertine dry granular and a preparation method thereof. The dry granular is prepared from feldspar, quartz, kaolin, calcined kaolin, iron phosphate, calcium phosphate, zinc oxide, viscous organic powder and poor organic powder. The internal surface of a hole of a metallic travertine brick obtained by sintering the metallic travertine dry granular provided by the invention has a glaze layer; a glaze surface is compact, is high in strength and has the advantages of being wear-resistant and not hiding dirt. The glaze surface of the hole of the metallic travertine brick is sunken and smooth and has a metallic texture; the glaze surface is abundant in color and clear in vein; the surface is plentiful and colorful to decorate; in addition, the vein of the glaze surface is designed by being matched with marble; the effect is more vivid and natural; the special effect that the metallic hole sparkles is achieved. The kinds of required raw materials are a few; the resource is abundant; the price is low; the preparation process is simple; the operability is high; the surface of a product has the effect that the metallic hole sparkles; the tone which is mainly cream-colored is broken; the color is richer and more colorful; the random and free characteristics of a natural stone are greatly restored, and no matter on a ground surface or a wall, the metallic travertine brick can be paved to obtain a quite good effect.

The invention discloses a probe, primer and kit for detecting AK4 gene polymorphism. The sequence of the probe is shown as SEQ ID NO.: 1, SEQ ID NO.: 4 or SEQ ID NO.: 7, or the two ends of each of the sequences of SEQ ID NO.: 1, SEQ ID NO.: 4 and SEQ ID NO.: 7 are subjected to group modification. The probe, primer and kit for detecting the AK4 gene polymorphism have the advantages of high sensitivity, good specificity, rapidness, high-throughput detection and the like during detection of AK4 gene polymorphism.

The invention discloses a PARMS marker based on SNP mutation of a coding region of a rice blast resistance gene Pid2 and an application. According to the invention, the difference of Pid2 in a high rice blast resistance material Y127 and an infected variety Nipponbare is detected by using first-generation sequencing, A->G mutation occurs at a 554bp site of the coding region of the gene, the difference of the two bases is introduced into a designed forward primer, and on the basis, two different fluorescence labeling universal primers are added, so that a fluorescence functional molecular marker of the rice blast resistance gene Pid2 based on the PARMS technology is developed, and application of the Pid2 gene to rice resistance molecular breeding is facilitated.

The invention provides a probe for detecting ACE2 gene polymorphism. The sequence of the probe is as shown in SEQ ID NO.: 1, or two ends of the sequence of SEQ ID NO.: 1 are modified by groups. The invention also provides a primer for detecting the polymorphism of the ACE2 gene, and the sequence of the primer is shown as SEQ ID NO.: 2 and SEQ ID NO.: 3. The probe and the primer for detecting the polymorphism of the ACE2 gene have the advantages of high sensitivity, good specificity, rapidness, high-throughput detection and the like when being used for detecting the polymorphism of the ACE2 gene.

The invention provides a probe for detecting tumor necrosis factor-alpha gene polymorphism. The sequence of the probe is as shown in SEQ ID NO.: 1, or two ends of the sequence of SEQ ID NO.: 1 are modified by groups. The invention also provides a primer for detecting the polymorphism of the tumor necrosis factor-alpha gene. The sequence of the primer is shown as SEQ ID NO.: 2 (primer 1) and SEQ ID NO.: 3 (primer 2). When the probe and the primer for detecting the tumor necrosis factor-alpha gene polymorphism are used for detecting the tumor necrosis factor-alpha gene polymorphism, the probe and the primer have the advantages of high sensitivity, good specificity, rapidness, high-throughput detection and the like.

The invention discloses a method for detecting ESR2 gene polymorphism. The method comprises the following steps: determining an ESR2 gene mutation site; designing and synthesizing a probe and a primeraccording to the mutation site; the method comprises the following steps: mixing a detected ESR2 target gene, a probe, a primer and an enzyme, carrying out fluorescent quantitative PCR, and monitoring a fluorescence signal; calculating a Tm value displayed by the hybridization peak, and judging the type of the ESR2 gene according to the Tm value; the invention also discloses a probe for detectingthe polymorphism of the ESR2 gene. The nucleotide sequence of the probe comprises SEQ ID NO. 3; the invention provides the probe and the method which are simple to operate and can be used for quicklydetecting the polymorphism of the ESR2 gene.

The invention relates to the technical field of molecular markers, and discloses a functional molecular marker of a rice nitrogen fertilizer utilization efficiency gene OsNPF3.1 and application of the functional molecular marker. The marker is located at the 8741040th nucleic acid site of the sixth chromosome of the rice OsNPF3.1 gene, and the basic group of the site is G or A and is correspondingly located at the 51st nucleic acid site of SEQ ID NO.1 in a nucleic acid sequence table. The invention further discloses application of the molecular marker in breeding or auxiliary breeding of rice varieties or strains related to the rice nitrogen utilization efficiency, and particularly application of the molecular marker in breeding or auxiliary breeding of rice with high/low rice nitrogen utilization rate. Through verification and research, the detection result of the OsNPF3.1 Chr68741040 is found to be high in reliability.

The invention discloses a bioremediation method for improving the transparency of a closed type small and micro water body, and particularly relates to the technical field of ecological remediation. The method comprises the following specific steps: 1, reducing the density of fishes in the water body, and reducing or removing native plants; 2, adding clams and potamogeton crispus stone buds to carry out turbidity reduction treatment on the water body; 3, recycling part of clams through a floating type ecological treatment device, and adding snails at the same time; 4, planting dwarf vallisneria natans; and 5, potting a small number of ornamental floating-leaved plants, and adding silver carp and bighead carp to improve the attractiveness of the water body. The water body is subjected to rapid turbidity reduction by using specific biological species and addition modes, and meanwhile, a stable and attractive ecological system is constructed according to the characteristics of the closed small water body, so that the pollution of chemical agents to the water body is avoided, the restoration speed of the transparency of the water body can be ensured, and meanwhile, the long-term clarity of the water body after the water body is restored is ensured.