54results about How to "Clear type" patented technology

The invention relates to a researching method of a dominant male genic male sterile material of hulless oat. The researching method specifically comprises the following steps: (1), implementing a characteristic and feature researching method of the sterile material; (2), implementing the research on a hybridization method; (3), implementing a genetic analysis researching method of the sterile material, namely, 1, surveying the fertility of an F1-generation sterile material; 2, tracking and surveying a fertile line separated from the F1-generation sterile material; 3, surveying fertility conditions of the sterile material under different ecological conditions; (4), implementing a sterility surveying method of the sterile material; and (5), implementing application of a genic male sterile material on a hulless oat, namely, 1, hybridization; 2, seed selection; and 3, breeding. By adopting a breeding procedure of a genic male sterile single-cross single-gene progressive summating method, a genic male sterile double-cross double-gene progressive summating method and a dynamic gene pool hybridization breeding method, a research on a recurrent selection method is developed. According to the researching method, the breeding level and the selecting efficiency of the hulless oat are increased, the increase of producing varieties, the high speed of producing the varieties, and the good quality of produced varieties are facilitated, the social benefit is large, the hybridization efficiency is improved, the cost is lowered, and the economic benefit is good.

The invention belongs to the technical field of liquor brewing, and provides an acid control and efficiency improvement method for Daqu delicate fragrance liquor in hot season, in order to solve the problems that the liquor produced by using Daqu as a saccharification fermentation agent goes rancidity easily in the hot season, and the liquor yield is affected. A Fen Daqu delicate fragrance Liquor brewage technology is employed, the consumption of Daqu is decreased, and rhizopus distiller's yeast, Saccharomyces cerevisiae and ester producing yeast are in cooperation; and sorghum is used as a raw material for fermentation liquor brewing. The dosage of the yeasts is as below: Daqu accounts for 2.5-7.5% of the raw material, rhizopus distiller's yeast accounts for 0.3-0.5% of the raw material, and Saccharomyces cerevisiae accounts for 0.05-0.01% of the raw material, and the ester producing yeast accounts for 0.05-0.1% of the raw material. The material and yeasts are subjected to ground tank fermentation for 13-15 days; and the initial fermentation temperature is controlled at 25-30 DEG C. The method effectively reduces the amount of Lactobacillus seed, ensures product flavor, presents novel style, greatly reduces the usage amount of the Fen liquor Daqu, lowers costs, and improves the efficiency of liquor brewing in the premise of effective acid control in the hot season. The liquor reserves the aroma function of Fen liquor Daqu, preserves complex composition, and guarantees the flavor characteristics of Fen liquor.

The invention discloses an HLA genotyping method based on a three-generation sequencing platform, which comprises the following steps: (1) performing PCR amplification on the HLA gene to be typed; (2) performing three-generation sequencing after the product obtained by PCR is detected to be qualified, Obtain the original data; (3) perform long sequence comparison between the original data and the reference gene sequence; (4) correct the sequencing errors after the comparison; (5) obtain the haplotype sequence by phase separation; (6) type judgment. Compared with the existing HLA genotyping methods, the HLA typing method of the present invention requires less computing resources, fast typing, and high resolution, and is useful for clinical transplant tissue matching, population genetics, anthropology and evolution, etc. Applied and basic research work is of great value.