PARMS marker based on rice brown planthopper resistance gene Bph14 and application

Abstract

The invention discloses a PARMS marker based on a rice brown planthopper resistance gene Bph14 and application of the PARMS marker, and particularly relates to a functional PARMS marker based on rice brown planthopper resistance gene Bph14 first exon SNP non-synonymous mutation and application of the functional PARMS marker. According to the invention, first-generation sequencing is utilized to detect the difference of Bph14 in a high brown planthopper resistance material Y009 and a susceptible brown planthopper variety Nipponbare, T->C mutation occurs at the 448bp site of the first exon of the gene to cause the change of phenylalanine into leucine so as to cause no function of Bph14, the difference of the two basic groups is introduced into a designed forward primer, and two different fluorescence labeling universal primers are added on the basis, so that the fluorescence functional molecular marker of the rice brown planthopper resistance gene Bph14 based on the PARMS technology is developed, and the application of the Bph14 gene in rice resistance molecular breeding is facilitated.

Description

【Technical field】 [0001] The invention belongs to the technical field of genetic engineering, and relates to a PARMS marker and application based on rice brown planthopper resistance gene Bph14, in particular to a functional PARMS marker and application based on non-synonymous mutation of the first exon SNP of rice brown planthopper resistance gene Bph14. 【Background technique】 [0002] The brown planthopper belongs to the planthopper family of the order Homoptera, and rice is the main host. The brown planthopper is one of the main pests harmful to rice production. The brown planthopper sucks rice vascular bundle sheath juice through the stylet, causing the base of the rice plant to turn black, lodging or even die in severe cases. The brown planthopper spreads rice grassy dwarf disease and leaf dwarf disease when feeding, and also promotes rice sheath blight. , Transmission of Sclerotinia sclerotiorum. With the change of farming methods and the large-scale promotion of high...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the disadvantages of marker detection for controlling brown planthopper in the prior art, such as long time-consuming, cumbersome operation, low efficiency, low degree of automation, use of toxic substances, and unsuitability for large-scale genotype analysis, the present invention provides a method based on rice brown planthopper resistance gene Bph14 The PARMS marker and its application are specifically based on the functional PARMS marker and application of the non-synonymous mutation of the first exon SNP of the rice brown planthopper resistance gene Bph14. Variety Nipponbare, it was found that a T→C mutation occurred at the 448bp site of exon 1 of the gene, resulting in a change from phenylalanine to leucine, resulting in the non-function of Bph14, which was introduced in the designed forward primer The difference of two bases, and on this basis, two different fluorescent marker universal primers were added to develop the fluorescent functional molecular marker of rice brown planthopper resistance gene Bph14 based on PARMS technology, which facilitates the application of Bph14 gene in molecular breeding of rice resistance

Images