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Venenum bufonis extract with anti-infection action and preparation method thereof

An extract and anti-infection technology, applied in the field of toad venom extract and its preparation, can solve the problems of restricting the application of toad venom drugs, unclear types and content, etc., and achieve good quality control, low cytotoxicity, active ingredient types and content clear effect

Active Publication Date: 2017-11-28
广州京泽健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the currently marketed drugs mainly use the extract of toad venom as the active ingredient, but the types of active ingredients in the extract and the content of each component are not clear. In addition, because the toxins in toad venom have certain toxicity ; this further limits the application of venomous drugs

Method used

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  • Venenum bufonis extract with anti-infection action and preparation method thereof
  • Venenum bufonis extract with anti-infection action and preparation method thereof
  • Venenum bufonis extract with anti-infection action and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 has the preparation of the toad venom extract of anti-infection effect

[0025] (1) Take 100 g of toad venom and add 1 L of ethanol aqueous solution with a volume fraction of 95%, soak at 25°C for 3 days, and filter to obtain the ethanol extract;

[0026] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxins;

[0027] The specific method for enriching the active ingredients of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first elute with water to remove Miscellaneous, and then use 80% ethanol to elute the non-polar macroporous resin column; collect ethanol eluted parts, concentrate and dry to obtain the extract.

[0028] After testing, the active ingredients of bufotoxins in the extract prepared in this example account...

Embodiment 2

[0032] Example 2 The preparation of the extract of toad venom with anti-infective effect

[0033] (1) Take 100 g of toad venom and add 1 L of ethanol aqueous solution with a volume fraction of 95%, soak at 25°C for 3 days, and filter to obtain the ethanol extract;

[0034] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxins;

[0035] The specific method for enriching the active ingredients of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first elute with water to remove Miscellaneous, and then use 60% ethanol to elute the non-polar macroporous resin column; collect ethanol eluted parts, concentrate and dry to obtain the extract.

[0036] After testing, the active ingredients of bufotoxins in the extract prepared in this example accounted...

Embodiment 3

[0037] Example 3 The preparation of the extract of toad venom with anti-infective effect

[0038] (1) Add 100 g of toad venom to 1 L of 80% ethanol aqueous solution, soak at 25°C for 2 days, and filter to obtain the ethanol extract;

[0039] (2) Concentrate the ethanol extract until it has no alcohol smell, and then use a non-polar macroporous resin column to enrich the active ingredients of bufotoxins;

[0040] The specific method for enriching the active ingredients of bufatoxins in the non-polar macroporous resin column is as follows: after loading the ethanol extract on the non-polar macroporous resin column (D101 macroporous resin column), first elute with water to remove Miscellaneous, and then use 40% ethanol to elute the non-polar macroporous resin column; collect ethanol eluted parts, concentrate and dry to obtain the extract.

[0041] After testing, the active ingredients of bufotoxins in the extract prepared in this example accounted for 45% of the total weight of ...

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Abstract

The invention relates to the technical field of traditional Chinese medicines and in particular discloses venenum bufonis extract with anti-infection action and a preparation method thereof. The venenum bufonis extract contains a phrynin effective component which contains the following components in parts by weight: 30-60 parts of arenobufagin, 35-60 parts of cinobufagin, 10-25 parts of telocinobufagin, 20-45 parts of bufotaline, 20-50 parts of cinobufotalin, 25-45 parts of bufalin, 15-40 parts of resibufogenin and 5-15 parts of hellebrigenin. The venenum bufonis extract provided by the invention is remarkable in anti-infection effect. In addition, the types and content of effective components of the extract are clear, the cytotoxicity is small, and quality control and medicinal safety of the extract are facilitated.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a toad venom extract with anti-infection effect and a preparation method thereof. Background technique [0002] Toad venom is the secretion of toad's post-auricular glands and epidermal glands, white milky liquid or light yellow serous, poisonous. Toad venom is extracted from the slurry, dried, colored, and can be used as medicine after being sealed and stored. It has many functions such as detoxification, analgesia, resuscitation, and antitumor, and is widely used. [0003] However, the currently marketed drugs mainly use the extract of toad venom as the active ingredient, but the types of active ingredients in the extract and the content of each component are not clear. In addition, because the toxins in toad venom have certain toxicity ; This further limits the application of venomous drugs. Therefore, it is of great significance to develop a toad veno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61P31/04A61P31/12
CPCA61K31/585A61K35/65A61K2300/00
Inventor 黄娇英
Owner 广州京泽健康科技有限公司
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