Functional molecular marker of rice nitrogen fertilizer utilization efficiency gene OsNPF3.1 and application

A functional molecule, rice nitrogen technology, applied in application, genomics, plant genetic improvement, etc., can solve the problems of inappropriate large-scale genotype analysis, long time-consuming marker detection, low degree of automation, etc., to avoid exposure to toxic Chemical substances, reliable test results, and less error-prone effects

Active Publication Date: 2022-05-31
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology

[0003] Commonly used molecular markers such as RFLP, RAPD, CAPS, dCAPS, AFLP, SSR, ISSR, STS, SRAP, IRAP and InDel, etc., can be used for linkage analysis and detection of target genes, but the detection of these markers is time-consuming, cumbersome and inefficient , low degree of automation and the use of toxic substances, etc., are not suitable for large-scale genotype analysis

Method used

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  • Functional molecular marker of rice nitrogen fertilizer utilization efficiency gene OsNPF3.1 and application
  • Functional molecular marker of rice nitrogen fertilizer utilization efficiency gene OsNPF3.1 and application

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Embodiment

[0035] The development and application of a functional molecular marker related to rice nitrogen use efficiency gene OsNPF3.1, the specific development test steps are as follows:

[0036] (1) Phenotypic identification of nitrogen use efficiency in rice: at 0.5 and 1.5 mM NH 4 Nitrogen fertilizer use efficiency of 132 indica rice and 22 japonica rice was identified in NO3 rice nutrient solution, and the plant height and dry matter weight were mainly examined.

[0037] (2) Analysis of variation sites in OsNPF3.1 genome region: Use the Rice SNP-SeekDatabase in the 3K database to download the variation sites in OsNPF3.1 genome region, and there are 31 SNPs in total.

[0038] (3) Gene analysis of 154 materials: According to the sequence information of 31 sites, 25 pairs of primers were designed. PARMS technology was used to genotype 132 indica rice and 22 japonica rice, and found that 18 loci could be analyzed normally.

[0039] (4) Correlation analysis: The genotype and phenotyp...

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Abstract

The invention relates to the technical field of molecular markers, and discloses a functional molecular marker of a rice nitrogen fertilizer utilization efficiency gene OsNPF3.1 and application of the functional molecular marker. The marker is located at the 8741040th nucleic acid site of the sixth chromosome of the rice OsNPF3.1 gene, and the basic group of the site is G or A and is correspondingly located at the 51st nucleic acid site of SEQ ID NO.1 in a nucleic acid sequence table. The invention further discloses application of the molecular marker in breeding or auxiliary breeding of rice varieties or strains related to the rice nitrogen utilization efficiency, and particularly application of the molecular marker in breeding or auxiliary breeding of rice with high/low rice nitrogen utilization rate. Through verification and research, the detection result of the OsNPF3.1 Chr68741040 is found to be high in reliability.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a functional molecular marker and application of rice nitrogen use efficiency gene OsNPF3.1 related to rice nitrogen use efficiency. 【Background technique】 [0002] Nitrogen is the mineral element with the greatest demand for plants, and it is also a key limiting factor for plant growth and development. In the practice of rice production, a large amount of nitrogen fertilizer has been one of the important measures to achieve its high yield. However, excessive nitrogen application not only increases the production cost, but also causes serious damage to the agricultural ecological environment. Studies have shown that cultivating new rice varieties with high nitrogen utilization is an effective way to reduce production costs, reduce environmental pollution, and greatly increase ecological benefits. A large number of molecular breeding practices have shown that analyzing the genetic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04G16B20/20G16B20/50
CPCC12Q1/6895A01H1/045G16B20/20G16B20/50C12Q2600/13C12Q2600/156Y02P60/21
Inventor 杨行海张宗琼夏秀忠农保选李丹婷曾宇吴艳艳荘洁蓝远芬
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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