Controllable preparation method of papain-wrapped gold nanocluster

Abstract

The invention relates to a controllable preparation method of a papain-wrapped gold nanocluster, and belongs to the technical field of nanotechnology. Papain is taken as a template, and the influenceof the change of the pH value in the reaction process on the optical performance of the gold nanocluster is explored; and by controlling the reaction pH, the temperature and the time, the gold nanoclusters capable of emitting green, blue and yellow fluorescence with the particle size of approximately equal to 1nm are correspondingly synthesized. The synthesis method is simple and green, and the synthesized gold nanocluster has good optical stability, high anti-light bleaching capability, and high catalytic performance and electroluminescence performance, and can be used as a good photochemistry, electrochemical signal probe or catalyst.

Description

technical field [0001] The invention belongs to the field of nanotechnology. Background technique [0002] Gold nanoclusters are composed of several to hundreds of gold atoms, and the particle size is usually less than 2nm. Because the size is close to the Fermi wavelength of electrons, the continuous state density is decomposed into discrete energy levels, so that it can occur in the metal core. Electronic transitions, including the inter-band electronic transition between the d band and the sp band and the intra-band electronic transition between the highest occupied orbit and the lowest unoccupied orbit of the sp band, so it has an obvious quantum size effect, which is specifically manifested when the gold nanometer When the size of the cluster decreases, the distance between discrete states in each band increases, resulting in a blue shift of the fluorescence emission peak. This characteristic is the theoretical basis for the controllable synthesis of gold nanoclusters. ...

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Problems solved by technology

[0004] However, the complex structure of many proteins and the variety of amino acids in the synthesis make it difficult to effectively control the complexing ability and reducibility of the reaction system. For example, the more common bovine serum albumin, pepsin, transferrin, etc. The fluorescence wavelength of gold nanoclusters is almost all greater than 700 nm, and the optical stability is poor. The reason is that the role of active amino acids such as cysteine, tyrosine, and tryptophan cannot be clarified, and many proteins are expensive. Therefore, looking for a structure Clear, low-cost protein templates are of great importance

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