Method for detecting content of chlortoluron in farmland water by using fluorescence carbon point and cadmium telluride quantum dot energy transferring technology

Abstract

The invention discloses a method for detecting the content of chlortoluron in farmland water by using a fluorescence carbon point and cadmium telluride quantum dot energy transferring technology. According to the method, a fluorescence carbon point is used as an energy transferring donor and a cadmium telluride quantum dot is used as an acceptor to form an energy transferring system with stable performance; the fluorescence carbon point is used for transferring energy to the cadmium telluride quantum dot so as to cause fluorescence enhancement of the cadmium telluride quantum dot. The chlortoluron is added and electrons on the surface of the cadmium telluride quantum dot are transferred to the chlortoluron to be in non-radiation combination with the chlortoluron, so that the fluorescent light of the cadmium telluride quantum dot is regularly quenched. The concentration of the chlortoluron has a good linear relation with a fluorescence quenching degree (F0 / F) in a range of 0.05-12.0 micrograms / liter; a linear regression equation is F0 / F=1.0+0.138c and the linearly dependent coefficient r is equal to 0.9979; the detection limit of the method is 0.017 microgram / liter. The method disclosed by the invention is simple, high in sensitivity and good in selectivity; the detection of the chlortoluron in a farmland water sample is very convenient and rapid.

Description

technical field

[0001] The invention relates to a method for rapidly detecting trace amounts of chlorotoluron in farmland water by utilizing fluorescent carbon dots and cadmium telluride quantum dot energy transfer technology. Background technique

[0002] Chlorotoluron is a phenylurea herbicide that can control the growth of annual grasses and broad-leaved grasses with high selectivity and high efficiency, and has become one of the most widely used phenylurea herbicides. Studies have shown that chlorotoluron has certain carcinogenicity and has direct toxic effects on aquatic plants. At present, the commonly used detection methods of chlorotoluron include chromatography, electrochemical method and enzyme-linked immunosorbent method. Although these methods have certain advantages, some of these methods are relatively expensive and some are complicated to operate, which limits their application in actual sample detection. Therefore, it is of great significance to develop a s...

Examples

Embodiment Construction

[0019] (1) Synthesis of water-soluble cadmium telluride quantum dots:

[0020] Add 0.048 g of tellurium powder, 0.120 g of sodium borohydride and 5 ml of secondary water into a 25 ml three-necked flask, mix well, and react for 20 minutes in a water bath at 65 °C with magnetic stirring to obtain purple transparent tellurium hydride Sodium aqueous solution, spare; under nitrogen protection, the concentration in 200 ml is 2.5×10 -3 Add 0.10 milliliters of analytically pure mercaptoacetic acid to the mol / liter cadmium chloride aqueous solution, adjust the pH to 9-11 with sodium hydroxide, quickly add 5.0 milliliters of the above aqueous sodium telluride hydride solution under strong stirring, and reflux at 95 °C for 2 hours, That is, a transparent cadmium telluride quantum dot solution is obtained.

[0021] (2) Synthesis of fluorescent carbon dots:

[0022] Add 0.2 grams of sodium citrate, 1.5 grams of ammonium bicarbonate and 10 milliliters of secondary water into the high-tem...

technical field

[0001] The invention relates to a method for rapidly detecting trace amounts of chlorotoluron in farmland water by utilizing fluorescent carbon dots and cadmium telluride quantum dot energy transfer technology. Background technique

[0002] Chlorotoluron is a phenylurea herbicide that can control the growth of annual grasses and broad-leaved grasses with high selectivity and high efficiency, and has become one of the most widely used phenylurea herbicides. Studies have shown that chlorotoluron has certain carcinogenicity and has direct toxic effects on aquatic plants. At present, the commonly used detection methods of chlorotoluron include chromatography, electrochemical method and enzyme-linked immunosorbent method. Although these methods have certain advantages, some of these methods are relatively expensive and some are complicated to operate, which limits their application in actual sample detection. Therefore, it is of great significance to develop a s...

Examples

Embodiment Construction

[0019] (1) Synthesis of water-soluble cadmium telluride quantum dots:

[0020] Add 0.048 g of tellurium powder, 0.120 g of sodium borohydride and 5 ml of secondary water into a 25 ml three-necked flask, mix well, and react for 20 minutes in a water bath at 65 °C with magnetic stirring to obtain purple transparent tellurium hydride Sodium aqueous solution, spare; under nitrogen protection, the concentration in 200 ml is 2.5×10 -3 Add 0.10 milliliters of analytically pure mercaptoacetic acid to the mol / liter cadmium chloride aqueous solution, adjust the pH to 9-11 with sodium hydroxide, quickly add 5.0 milliliters of the above aqueous sodium telluride hydride solution under strong stirring, and reflux at 95 °C for 2 hours, That is, a transparent cadmium telluride quantum dot solution is obtained.

[0021] (2) Synthesis of fluorescent carbon dots:

[0022] Add 0.2 grams of sodium citrate, 1.5 grams of ammonium bicarbonate and 10 milliliters of secondary water into the high-tem...

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